Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer
Li, Y
Xu, X
Song, L
Hou, Y
Li, Z
Tsang, S
Li, F
Im, KM
Wu, K
Wu, H
Ye, X
Li, G
Wang, L
Zhang, B
Liang, J
Xie, W
Wu, R
Jiang, H
Liu, X
Yu, C
Zheng, H
Jian, M
Nie, L
Wan, L
Shi, M
Sun, X
Tang, A
Guo, G
Gui, Y
Cai, Z
Li, J
Wang, W
Lu, Z
Zhang, X
Bolund, L
Kristiansen, K
Wang, J
Yang, H
Dean, M
- Publication Type:
- Journal Article
- Citation:
- GigaScience, 2012, 61 (1)
- Issue Date:
- 2012-08-14
Recently Added
Filename | Description | Size | |||
---|---|---|---|---|---|
Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer.pdf | Published Version | 521.31 kB | Adobe PDF |
Copyright Clearance Process
- Recently Added
- In Progress
- Open Access
This item is new to OPUS and is not currently available.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Li, Y | en_US |
dc.contributor.author | Xu, X | en_US |
dc.contributor.author | Song, L | en_US |
dc.contributor.author | Hou, Y | en_US |
dc.contributor.author | Li, Z | en_US |
dc.contributor.author | Tsang, S | en_US |
dc.contributor.author | Li, F | en_US |
dc.contributor.author | Im, KM | en_US |
dc.contributor.author | Wu, K | en_US |
dc.contributor.author |
Wu, H |
en_US |
dc.contributor.author | Ye, X | en_US |
dc.contributor.author | Li, G | en_US |
dc.contributor.author | Wang, L | en_US |
dc.contributor.author | Zhang, B | en_US |
dc.contributor.author | Liang, J | en_US |
dc.contributor.author | Xie, W | en_US |
dc.contributor.author | Wu, R | en_US |
dc.contributor.author | Jiang, H | en_US |
dc.contributor.author | Liu, X | en_US |
dc.contributor.author | Yu, C | en_US |
dc.contributor.author | Zheng, H | en_US |
dc.contributor.author | Jian, M | en_US |
dc.contributor.author | Nie, L | en_US |
dc.contributor.author | Wan, L | en_US |
dc.contributor.author | Shi, M | en_US |
dc.contributor.author | Sun, X | en_US |
dc.contributor.author | Tang, A | en_US |
dc.contributor.author | Guo, G | en_US |
dc.contributor.author | Gui, Y | en_US |
dc.contributor.author | Cai, Z | en_US |
dc.contributor.author | Li, J | en_US |
dc.contributor.author | Wang, W | en_US |
dc.contributor.author | Lu, Z | en_US |
dc.contributor.author | Zhang, X | en_US |
dc.contributor.author | Bolund, L | en_US |
dc.contributor.author | Kristiansen, K | en_US |
dc.contributor.author | Wang, J | en_US |
dc.contributor.author | Yang, H | en_US |
dc.contributor.author | Dean, M | en_US |
dc.date.available | 2012-08-02 | en_US |
dc.date.issued | 2012-08-14 | en_US |
dc.identifier.citation | GigaScience, 2012, 61 (1) | en_US |
dc.identifier.uri | http://hdl.handle.net/10453/134062 | |
dc.description.abstract | Background: Cancers arise through an evolutionary process in which cell populations are subjected to selection; however, to date, the process of bladder cancer, which is one of the most common cancers in the world, remains unknown at a single-cell level. Results: We carried out single-cell exome sequencing of 66 individual tumor cells from a muscle-invasive bladder transitional cell carcinoma (TCC). Analyses of the somatic mutant allele frequency spectrum and clonal structure revealed that the tumor cells were derived from a single ancestral cell, but that subsequent evolution occurred, leading to two distinct tumor cell subpopulations. By analyzing recurrently mutant genes in an additional cohort of 99 TCC tumors, we identified genes that might play roles in the maintenance of the ancestral clone and in the muscle-invasive capability of subclones of this bladder cancer, respectively. Conclusions: This work provides a new approach of investigating the genetic details of bladder tumoral changes at the single-cell level and a new method for assessing bladder cancer evolution at a cell-population level. © 2012 Li et al.; licensee BioMed Central Ltd. | en_US |
dc.relation.ispartof | GigaScience | en_US |
dc.relation.isbasedon | 10.1186/2047-217X-1-12 | en_US |
dc.title | Single-cell sequencing analysis characterizes common and cell-lineage-specific mutations in a muscle-invasive bladder cancer | en_US |
dc.type | Journal Article | |
utslib.citation.volume | 1 | en_US |
utslib.citation.volume | 61 | en_US |
pubs.embargo.period | Not known | en_US |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Students | |
utslib.copyright.status | recently_added | |
pubs.issue | 1 | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 61 | en_US |
Abstract:
Background: Cancers arise through an evolutionary process in which cell populations are subjected to selection; however, to date, the process of bladder cancer, which is one of the most common cancers in the world, remains unknown at a single-cell level. Results: We carried out single-cell exome sequencing of 66 individual tumor cells from a muscle-invasive bladder transitional cell carcinoma (TCC). Analyses of the somatic mutant allele frequency spectrum and clonal structure revealed that the tumor cells were derived from a single ancestral cell, but that subsequent evolution occurred, leading to two distinct tumor cell subpopulations. By analyzing recurrently mutant genes in an additional cohort of 99 TCC tumors, we identified genes that might play roles in the maintenance of the ancestral clone and in the muscle-invasive capability of subclones of this bladder cancer, respectively. Conclusions: This work provides a new approach of investigating the genetic details of bladder tumoral changes at the single-cell level and a new method for assessing bladder cancer evolution at a cell-population level. © 2012 Li et al.; licensee BioMed Central Ltd.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph