Factors Affecting Corneal Organ Culture Contamination: A 6-year Study at the New South Wales Tissue Bank

Ling, MLH; Wells, M; Petsoglou, C; Luo, K; Georges, P; Devasahayam, R; Hodge, C; Treloggen, J; Sutton, G; Zhu, M

Factors Affecting Corneal Organ Culture Contamination: A 6-year Study at the New South Wales Tissue Bank

Ling, MLH Wells, M Petsoglou, C Luo, K Georges, P Devasahayam, R Hodge, C

Treloggen, J Sutton, G Zhu, M

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Publication Type:: Journal Article
Citation:: Cornea, 2019, 38 (7), pp. 829 - 835
Issue Date:: 2019-07-01

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Field	Value	Language
dc.contributor.author	Ling, MLH	en_US
dc.contributor.author	Wells, M	en_US
dc.contributor.author	Petsoglou, C	en_US
dc.contributor.author	Luo, K	en_US
dc.contributor.author	Georges, P	en_US
dc.contributor.author	Devasahayam, R	en_US
dc.contributor.author	Hodge, C https://orcid.org/0000-0002-0516-6486	en_US
dc.contributor.author	Treloggen, J	en_US
dc.contributor.author	Sutton, G	en_US
dc.contributor.author	Zhu, M	en_US
dc.date.issued	2019-07-01	en_US
dc.identifier.citation	Cornea, 2019, 38 (7), pp. 829 - 835	en_US
dc.identifier.issn	0277-3740	en_US
dc.identifier.uri	http://hdl.handle.net/10453/135321
dc.description.abstract	© 2019 Wolters Kluwer Health, Inc. All rights reserved. Purpose:To report the rate of microbial contamination and analyze possible risk factors for contamination of banked corneas stored using the organ culture method.Methods:Data from the New South Wales Tissue Banks incorporating the Lions NSW Eye Bank, between September 1, 2011, and November 30, 2017, were reviewed retrospectively. All corneas collected during this period and stored in organ culture storage media were tested for microbial contamination. The influence of potential factors on the rate of contamination was analyzed using the χ2 test and logistic regression using generalized estimating equations.Results:A total of 4410 corneas were included in this study, of which 110 were medium culture positive, representing a microbial contamination rate of 2.5%. The main contaminants were Candida species followed by Staphylococcus species. Corneal tissue collected in summer and autumn had a significantly higher contamination rate (P = 0.006). All other factors studied were not shown to have a statistically significant association with contamination after accounting for within-pair correlation and confounders.Conclusions:A relatively low contamination rate of 2.5% observed in our study reflects the stringent laboratory protocols, strict donor selection criteria, and high level of experience among staff at the Lions NSW Eye Bank. Our study demonstrated that the season of collection had a strong association with the rate of organ culture contamination. Because Candida species contributed the largest percentage of contamination, specific measures to reduce and eliminate fungal proliferation should be considered by eye banks particularly in warm seasons.	en_US
dc.relation.ispartof	Cornea	en_US
dc.relation.isbasedon	10.1097/ICO.0000000000001936	en_US
dc.subject.classification	Ophthalmology & Optometry	en_US
dc.subject.mesh	Cornea	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Fungi	en_US
dc.subject.mesh	Eye Infections, Bacterial	en_US
dc.subject.mesh	Organ Preservation	en_US
dc.subject.mesh	Organ Culture Techniques	en_US
dc.subject.mesh	Logistic Models	en_US
dc.subject.mesh	Risk Factors	en_US
dc.subject.mesh	Retrospective Studies	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Aged	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Tissue Donors	en_US
dc.subject.mesh	Eye Banks	en_US
dc.subject.mesh	New South Wales	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.title	Factors Affecting Corneal Organ Culture Contamination: A 6-year Study at the New South Wales Tissue Bank	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	38	en_US
utslib.for	1113 Opthalmology and Optometry	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	38	en_US

Abstract:

© 2019 Wolters Kluwer Health, Inc. All rights reserved. Purpose:To report the rate of microbial contamination and analyze possible risk factors for contamination of banked corneas stored using the organ culture method.Methods:Data from the New South Wales Tissue Banks incorporating the Lions NSW Eye Bank, between September 1, 2011, and November 30, 2017, were reviewed retrospectively. All corneas collected during this period and stored in organ culture storage media were tested for microbial contamination. The influence of potential factors on the rate of contamination was analyzed using the χ2 test and logistic regression using generalized estimating equations.Results:A total of 4410 corneas were included in this study, of which 110 were medium culture positive, representing a microbial contamination rate of 2.5%. The main contaminants were Candida species followed by Staphylococcus species. Corneal tissue collected in summer and autumn had a significantly higher contamination rate (P = 0.006). All other factors studied were not shown to have a statistically significant association with contamination after accounting for within-pair correlation and confounders.Conclusions:A relatively low contamination rate of 2.5% observed in our study reflects the stringent laboratory protocols, strict donor selection criteria, and high level of experience among staff at the Lions NSW Eye Bank. Our study demonstrated that the season of collection had a strong association with the rate of organ culture contamination. Because Candida species contributed the largest percentage of contamination, specific measures to reduce and eliminate fungal proliferation should be considered by eye banks particularly in warm seasons.

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http://hdl.handle.net/10453/135321