Fluorescence excitation-emission spectroscopy: An analytical technique to monitor drugs of addiction in wastewater

Yadav, MK; Aryal, R; Short, MD; Saint, CP

Fluorescence excitation-emission spectroscopy: An analytical technique to monitor drugs of addiction in wastewater

Yadav, MK Aryal, R

Short, MD Saint, CP

Permalink

Publication Type:: Journal Article
Citation:: Water (Switzerland), 2019, 11 (2)
Issue Date:: 2019-02-01

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published VersionAdobe PDF (2.08 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Yadav, MK	en_US
dc.contributor.author	Aryal, R https://orcid.org/0000-0001-7348-4649	en_US
dc.contributor.author	Short, MD	en_US
dc.contributor.author	Saint, CP	en_US
dc.date.issued	2019-02-01	en_US
dc.identifier.citation	Water (Switzerland), 2019, 11 (2)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/135342
dc.description.abstract	© 2019 by the authors. Emerging contaminants of concern have become a serious issue for the scientific community and society more broadly in recent years due to their increasingly widespread environmental distribution and largely unknown environmental and human health impacts. This study aimed to explore the use of fluorescence excitation-emission (F-EEM) spectroscopy as an alternative analytical method to evaluate the presence of key drugs of addiction (benzoylecgonine, methamphetamine, MDMA, codeine and morphine) in wastewater treatment plants. The chemicals of interest from wastewater were extracted by mixed-mode solid phase extraction and quantified using liquid chromatography tandem mass spectrometry. The same wastewater samples were also analysed by a fluorescence spectrophotometer for fluorescence spectra at wavelengths 280-600 nm (emission) and 200-600 nm (excitation). The study also investigated the relevance of different methods for interpreting F-EEM matrices data including parallel factor analysis (PARAFAC) modelling and fluorescence regional integration technique. PARAFAC identified four components, and among them, component C2, identified at the λex/λem = 275/340 nm wavelength associated with proteinaceous compounds most likely related to tryptophan amino acid, showed significant correlation with codeine removal. MDMA and morphine were not correlated to any of the fluorescence regions. The fluorescence regions related to aromatic protein-like fluorescence were correlated significantly with drug concentration and so may offer a suitable alternative approach for monitoring drugs including benzoylecgonine, methamphetamine and codeine.	en_US
dc.relation.ispartof	Water (Switzerland)	en_US
dc.relation.isbasedon	10.3390/w11020377	en_US
dc.title	Fluorescence excitation-emission spectroscopy: An analytical technique to monitor drugs of addiction in wastewater	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	11	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
utslib.copyright.status	open_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	11	en_US

Abstract:

© 2019 by the authors. Emerging contaminants of concern have become a serious issue for the scientific community and society more broadly in recent years due to their increasingly widespread environmental distribution and largely unknown environmental and human health impacts. This study aimed to explore the use of fluorescence excitation-emission (F-EEM) spectroscopy as an alternative analytical method to evaluate the presence of key drugs of addiction (benzoylecgonine, methamphetamine, MDMA, codeine and morphine) in wastewater treatment plants. The chemicals of interest from wastewater were extracted by mixed-mode solid phase extraction and quantified using liquid chromatography tandem mass spectrometry. The same wastewater samples were also analysed by a fluorescence spectrophotometer for fluorescence spectra at wavelengths 280-600 nm (emission) and 200-600 nm (excitation). The study also investigated the relevance of different methods for interpreting F-EEM matrices data including parallel factor analysis (PARAFAC) modelling and fluorescence regional integration technique. PARAFAC identified four components, and among them, component C2, identified at the λex/λem = 275/340 nm wavelength associated with proteinaceous compounds most likely related to tryptophan amino acid, showed significant correlation with codeine removal. MDMA and morphine were not correlated to any of the fluorescence regions. The fluorescence regions related to aromatic protein-like fluorescence were correlated significantly with drug concentration and so may offer a suitable alternative approach for monitoring drugs including benzoylecgonine, methamphetamine and codeine.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/135342