Erlotinib loaded chitosan nanoparticles: Formulation, physicochemical characterization and cytotoxic potential

Pandey, P; Dua, K; Dureja, H

Erlotinib loaded chitosan nanoparticles: Formulation, physicochemical characterization and cytotoxic potential

Pandey, P Dua, K

Dureja, H

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Publication Type:: Journal Article
Citation:: International Journal of Biological Macromolecules, 2019, 139 pp. 1304 - 1316
Issue Date:: 2019-10-15

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Field	Value	Language
dc.contributor.author	Pandey, P	en_US
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159	en_US
dc.contributor.author	Dureja, H	en_US
dc.date.available	2019-08-08	en_US
dc.date.issued	2019-10-15	en_US
dc.identifier.citation	International Journal of Biological Macromolecules, 2019, 139 pp. 1304 - 1316	en_US
dc.identifier.issn	0141-8130	en_US
dc.identifier.uri	http://hdl.handle.net/10453/136280
dc.description.abstract	© 2019 Elsevier B.V. Cancer is the major cause of mortality and morbidity throughout the world where >10 million patients with new cases diagnosed every year. The objective of this study was to prepare and evaluate erlotinib loaded chitosan nanoparticles for their anticancer potential. Also, to study the effect of various formulation variables on prepared nanoparticles using box-behnken design. Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using the spray drying technique. It was found that batch SNP-9 has a maximum loading capacity (74.45 ± 0.34%) and entrapment efficiency (43 ± 0.57%) with a particle size 170.2 nm. Analysis of variance (ANOVA) was applied on the particle size, entrapment efficiency and % cumulative drug release to study the fitting and the significance of the model. The batch SNP-9 showed 89.46% and 40.12% drug release after 24 h in 0.1 N HCl and Phosphate Buffer (pH 6.8), respectively. The IC50 value of SNP-9 evaluated on A549 Lung cancer cells was found to be 4.41 μM. The optimized formulation was found stable after the six-month study as no considerable transformation was detected. The optimized formulation released erlotinib slowly in comparison to the marketed tablet formulation. Erlotinib loaded chitosan nanoparticles were prepared successfully using spray drying technique with suitable particle size, entrapment efficiency, drug release. The synthesized and optimized nanoparticles were found to possess activity against cancer cells when evaluated in-vitro.	en_US
dc.relation.ispartof	International Journal of Biological Macromolecules	en_US
dc.relation.isbasedon	10.1016/j.ijbiomac.2019.08.084	en_US
dc.subject.classification	Polymers	en_US
dc.title	Erlotinib loaded chitosan nanoparticles: Formulation, physicochemical characterization and cytotoxic potential	en_US
dc.type	Journal Article
utslib.citation.volume	139	en_US
utslib.for	100701 Environmental Nanotechnology	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	139	en_US

Abstract:

© 2019 Elsevier B.V. Cancer is the major cause of mortality and morbidity throughout the world where >10 million patients with new cases diagnosed every year. The objective of this study was to prepare and evaluate erlotinib loaded chitosan nanoparticles for their anticancer potential. Also, to study the effect of various formulation variables on prepared nanoparticles using box-behnken design. Erlotinib loaded chitosan nanoparticles were prepared by ionic gelation method using the spray drying technique. It was found that batch SNP-9 has a maximum loading capacity (74.45 ± 0.34%) and entrapment efficiency (43 ± 0.57%) with a particle size 170.2 nm. Analysis of variance (ANOVA) was applied on the particle size, entrapment efficiency and % cumulative drug release to study the fitting and the significance of the model. The batch SNP-9 showed 89.46% and 40.12% drug release after 24 h in 0.1 N HCl and Phosphate Buffer (pH 6.8), respectively. The IC50 value of SNP-9 evaluated on A549 Lung cancer cells was found to be 4.41 μM. The optimized formulation was found stable after the six-month study as no considerable transformation was detected. The optimized formulation released erlotinib slowly in comparison to the marketed tablet formulation. Erlotinib loaded chitosan nanoparticles were prepared successfully using spray drying technique with suitable particle size, entrapment efficiency, drug release. The synthesized and optimized nanoparticles were found to possess activity against cancer cells when evaluated in-vitro.

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http://hdl.handle.net/10453/136280