High dissolved oxygen selection against nitrospira sublineage i in full-scale activated sludge

Law, Y; Matysik, A; Chen, X; Swa Thi, S; Ngoc Nguyen, TQ; Qiu, G; Natarajan, G; Williams, RBH; Ni, BJ; Seviour, TW; Wuertz, S

High dissolved oxygen selection against nitrospira sublineage i in full-scale activated sludge

Law, Y Matysik, A Chen, X Swa Thi, S Ngoc Nguyen, TQ Qiu, G Natarajan, G Williams, RBH Ni, BJ

Seviour, TW Wuertz, S

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Publication Type:: Journal Article
Citation:: Environmental Science and Technology, 2019, 53 (14), pp. 8157 - 8166
Issue Date:: 2019-07-16

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Field	Value	Language
dc.contributor.author	Law, Y	en_US
dc.contributor.author	Matysik, A	en_US
dc.contributor.author	Chen, X	en_US
dc.contributor.author	Swa Thi, S	en_US
dc.contributor.author	Ngoc Nguyen, TQ	en_US
dc.contributor.author	Qiu, G	en_US
dc.contributor.author	Natarajan, G	en_US
dc.contributor.author	Williams, RBH	en_US
dc.contributor.author	Ni, BJ https://orcid.org/0000-0002-1129-7837	en_US
dc.contributor.author	Seviour, TW	en_US
dc.contributor.author	Wuertz, S	en_US
dc.date.issued	2019-07-16	en_US
dc.identifier.citation	Environmental Science and Technology, 2019, 53 (14), pp. 8157 - 8166	en_US
dc.identifier.issn	0013-936X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/138701
dc.description.abstract	© 2019 American Chemical Society. A single Nitrospira sublineage I OTU was found to perform nitrite oxidation in full-scale domestic wastewater treatment plants (WWTPs) in the tropics. This taxon had an apparent oxygen affinity constant lower than that of the full-scale domestic activated sludge cohabitating ammonium oxidizing bacteria (AOB) (0.09 ± 0.02 g O2 m-3 versus 0.3 ± 0.03 g O2 m-3). Thus, nitrite oxidizing bacteria (NOB) may in fact thrive under conditions of low oxygen supply. Low dissolved oxygen (DO) conditions selected for and high aeration inhibited the NOB in a long-term lab-scale reactor. The relative abundance of Nitrospira sublineage I gradually decreased with increasing DO until it was washed out. Nitritation was sustained even after the DO was lowered subsequently. The morphologies of AOB and NOB microcolonies responded to DO levels in accordance with their oxygen affinities. NOB formed densely packed spherical clusters with a low surface area-to-volume ratio compared to the Nitrosomonas-like AOB clusters, which maintained a porous and nonspherical morphology. In conclusion, the effect of oxygen on AOB/NOB population dynamics depends on which OTU predominates given that oxygen affinities are species-specific, and this should be elucidated when devising operating strategies to achieve mainstream partial nitritation.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT160100195
dc.relation.ispartof	Environmental Science and Technology	en_US
dc.relation.isbasedon	10.1021/acs.est.9b00955	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Nitrites	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	Ammonia	en_US
dc.subject.mesh	Bioreactors	en_US
dc.subject.mesh	Sewage	en_US
dc.subject.mesh	Oxidation-Reduction	en_US
dc.title	High dissolved oxygen selection against nitrospira sublineage i in full-scale activated sludge	en_US
dc.type	Journal Article
utslib.citation.volume	14	en_US
utslib.citation.volume	53	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access
pubs.issue	14	en_US
pubs.publication-status	Published	en_US
pubs.volume	53	en_US

Abstract:

© 2019 American Chemical Society. A single Nitrospira sublineage I OTU was found to perform nitrite oxidation in full-scale domestic wastewater treatment plants (WWTPs) in the tropics. This taxon had an apparent oxygen affinity constant lower than that of the full-scale domestic activated sludge cohabitating ammonium oxidizing bacteria (AOB) (0.09 ± 0.02 g O2 m-3 versus 0.3 ± 0.03 g O2 m-3). Thus, nitrite oxidizing bacteria (NOB) may in fact thrive under conditions of low oxygen supply. Low dissolved oxygen (DO) conditions selected for and high aeration inhibited the NOB in a long-term lab-scale reactor. The relative abundance of Nitrospira sublineage I gradually decreased with increasing DO until it was washed out. Nitritation was sustained even after the DO was lowered subsequently. The morphologies of AOB and NOB microcolonies responded to DO levels in accordance with their oxygen affinities. NOB formed densely packed spherical clusters with a low surface area-to-volume ratio compared to the Nitrosomonas-like AOB clusters, which maintained a porous and nonspherical morphology. In conclusion, the effect of oxygen on AOB/NOB population dynamics depends on which OTU predominates given that oxygen affinities are species-specific, and this should be elucidated when devising operating strategies to achieve mainstream partial nitritation.

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http://hdl.handle.net/10453/138701