Expression of HGF and c-met proteins in human keratoconus corneas

You, J; Wen, L; Roufas, A; Hodge, C; Sutton, G; Madigan, MC

Expression of HGF and c-met proteins in human keratoconus corneas

You, J Wen, L Roufas, A Hodge, C

Sutton, G Madigan, MC

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Publication Type:: Journal Article
Citation:: Journal of Ophthalmology, 2015, 2015
Issue Date:: 2015-01-01

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Field	Value	Language
dc.contributor.author	You, J	en_US
dc.contributor.author	Wen, L	en_US
dc.contributor.author	Roufas, A	en_US
dc.contributor.author	Hodge, C https://orcid.org/0000-0002-0516-6486	en_US
dc.contributor.author	Sutton, G	en_US
dc.contributor.author	Madigan, MC	en_US
dc.date.accessioned	2020-04-02T02:01:49Z
dc.date.available	2015-11-12	en_US
dc.date.available	2020-04-02T02:01:49Z
dc.date.issued	2015-01-01	en_US
dc.identifier.citation	Journal of Ophthalmology, 2015, 2015	en_US
dc.identifier.issn	2090-004X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/139736
dc.description.abstract	© 2015 Jingjing You et al. Keratoconus (KC) is a progressive degenerative inflammatory-related disease of the human cornea leading to decreased visual function. The pathogenesis of KC remains to be understood. Recent genetic studies indicate that gene variants of an inflammation-related molecule, hepatocyte growth factor (HGF), are associated with an increased susceptibility for developing KC. However HGF protein expression in KC has not been explored. In this initial study, we investigated late-stage KC and control corneas for the expression of HGF and its receptor mesenchymal-epithelial transition factor (c-Met/Met). KC buttons (8 mm diameter) (n = 10) and whole control corneas (n = 6) were fixed in 10% formalin or 2% paraformaldehyde, paraffin embedded and sectioned. Sections were immunolabelled with HGF and c-Met antibodies, visualised using immunofluorescence, and examined with scanning laser confocal microscopy. Semiquantitative grading was used to compare HGF and c-Met immunostaining in KC and control corneas. Overall, KC corneas showed increased HGF and c-Met immunostaining compared to controls. KC corneal epithelium displayed heterogeneous moderate-to-strong immunoreactivity for HGF and c-Met, particularly in the basal epithelium adjacent to the cone area. Taken together with the recent genetic studies, our results further support a possible role for HGF/c-Met in the pathogenesis of KC.	en_US
dc.relation.ispartof	Journal of Ophthalmology	en_US
dc.relation.isbasedon	10.1155/2015/852986	en_US
dc.title	Expression of HGF and c-met proteins in human keratoconus corneas	en_US
dc.type	Journal Article
utslib.citation.volume	2015	en_US
utslib.for	1113 Opthalmology and Optometry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	2015	en_US

Abstract:

© 2015 Jingjing You et al. Keratoconus (KC) is a progressive degenerative inflammatory-related disease of the human cornea leading to decreased visual function. The pathogenesis of KC remains to be understood. Recent genetic studies indicate that gene variants of an inflammation-related molecule, hepatocyte growth factor (HGF), are associated with an increased susceptibility for developing KC. However HGF protein expression in KC has not been explored. In this initial study, we investigated late-stage KC and control corneas for the expression of HGF and its receptor mesenchymal-epithelial transition factor (c-Met/Met). KC buttons (8 mm diameter) (n = 10) and whole control corneas (n = 6) were fixed in 10% formalin or 2% paraformaldehyde, paraffin embedded and sectioned. Sections were immunolabelled with HGF and c-Met antibodies, visualised using immunofluorescence, and examined with scanning laser confocal microscopy. Semiquantitative grading was used to compare HGF and c-Met immunostaining in KC and control corneas. Overall, KC corneas showed increased HGF and c-Met immunostaining compared to controls. KC corneal epithelium displayed heterogeneous moderate-to-strong immunoreactivity for HGF and c-Met, particularly in the basal epithelium adjacent to the cone area. Taken together with the recent genetic studies, our results further support a possible role for HGF/c-Met in the pathogenesis of KC.

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http://hdl.handle.net/10453/139736