Optimization of dilute acid and enzymatic hydrolysis for dark fermentative hydrogen production from the empty fruit bunch of oil palm

Publisher:
PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:
Journal Article
Citation:
International Journal of Hydrogen Energy, 2019, 44, (4), pp. 2191-2202
Issue Date:
2019-01-22
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1-s2.0-S036031991832514X-main.pdfPublished version2.67 MB
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© 2018 Hydrogen Energy Publications LLC Pretreatment of the empty fruit brunch (EFB) from oil palm was investigated for H 2 fermentation. The EFB was hydrolyzed at various temperatures, H 2 SO 4 concentrations, and reaction times. Subsequently, the acid-hydrolysate underwent enzymatic saccharification under various temperature, pH, and enzymatic loading conditions. Response surface methodology derived the optimum sugar concentration (SC), hydrogen production rate (HPR), and hydrogen yield (HY) as 28.30 g L −1 , 2601.24 mL H 2 L −1 d −1 , and 275.75 mL H 2 g −1 total sugar (TS), respectively, at 120 °C, 60 min of reaction, and 6 vol% H 2 SO 4 , with the combined severity factor of 1.75. Enzymatic hydrolysis enhanced the SC, HY, and HPR to 34.52 g L −1 , 283.91 mL H 2 g −1 TS, and 3266.86 mL H 2 L −1 d −1 , respectively, at 45 °C, pH 5.0, and 1.17 mg enzyme mL −1 . Dilute acid hydrolysis would be a viable pretreatment for biohydrogen production from EFB. Subsequent enzymatic hydrolysis can be performed if enhanced HPR is required.
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