Simultaneous HPTLC densitometric estimation of KBA and AKBA from Boswellia serrata

Mehta, M; Garg, M; Dua, K; Satija, S

Simultaneous HPTLC densitometric estimation of KBA and AKBA from Boswellia serrata

Mehta, M Garg, M Dua, K

Satija, S

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Publisher:: BENTHAM SCIENCE PUBL LTD
Publication Type:: Journal Article
Citation:: Current Analytical Chemistry, 2019, 15, (1), pp. 84-91
Issue Date:: 2019-01-01

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Field	Value	Language
dc.contributor.author	Mehta, M
dc.contributor.author	Garg, M
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Satija, S https://orcid.org/0000-0003-4702-6534
dc.date.accessioned	2020-05-28T06:41:08Z
dc.date.available	2020-05-28T06:41:08Z
dc.date.issued	2019-01-01
dc.identifier.citation	Current Analytical Chemistry, 2019, 15, (1), pp. 84-91
dc.identifier.issn	1573-4110
dc.identifier.issn	1875-6727
dc.identifier.uri	http://hdl.handle.net/10453/140980
dc.description.abstract	© 2019 Bentham Science Publishers. Background: Boswellic acids (BAs) are extracted from oleo gum of Boswellia serrata and are utilized as potential anti-inflammatory, hypolipidemic, immunomodulatory and antitumor specialists. The present examination was meant to assess KBA and AKBA in Boswellia serrata separate by High-Performance Thin Layer Chromatography (HPTLC). Methods: The separation of bioactive compounds was performed utilizing mobile phase glacial acetic acid, n-hexane, ethyl acetate and toluene (0.3: 1: 8: 2) (v/v/v/v) and distinguished at wavelength 254 nm. The technique was approved for linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and so forth by International Conference on Harmonization guidelines. Results: The calibration range was observed to be 2-14 µg/band for both the bioactive compounds. KBA was isolated with an R f estimation of 0.39 ± 0.02 and AKBA with an R f estimation of 0.42 ± 0.02. The accuracy was seen to be as high as 99.17% and 97.42 for KBA and KBA, respectively. The percentage RSD value for intra-day and between day varieties was under 2%. The system indicated high affectability and specificity. Conclusion: The developed HPTLC method was simple, precise, robust, specific, rapid, and costeffective and could be used for quality control analysis and quantification of KBA and AKBA in different herbal formulations containing the plant species.
dc.language	English
dc.publisher	BENTHAM SCIENCE PUBL LTD
dc.relation.ispartof	Current Analytical Chemistry
dc.relation.isbasedon	10.2174/1573411014666180704123521
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0301 Analytical Chemistry
dc.subject.classification	Analytical Chemistry
dc.title	Simultaneous HPTLC densitometric estimation of KBA and AKBA from Boswellia serrata
dc.type	Journal Article
utslib.citation.volume	15
utslib.for	0301 Analytical Chemistry
pubs.organisational-group	/University of Technology Sydney/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-05-28T06:41:04Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	15
utslib.start-page	84
utslib.citation.issue	1

Abstract:

© 2019 Bentham Science Publishers. Background: Boswellic acids (BAs) are extracted from oleo gum of Boswellia serrata and are utilized as potential anti-inflammatory, hypolipidemic, immunomodulatory and antitumor specialists. The present examination was meant to assess KBA and AKBA in Boswellia serrata separate by High-Performance Thin Layer Chromatography (HPTLC). Methods: The separation of bioactive compounds was performed utilizing mobile phase glacial acetic acid, n-hexane, ethyl acetate and toluene (0.3: 1: 8: 2) (v/v/v/v) and distinguished at wavelength 254 nm. The technique was approved for linearity, precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and so forth by International Conference on Harmonization guidelines. Results: The calibration range was observed to be 2-14 µg/band for both the bioactive compounds. KBA was isolated with an R f estimation of 0.39 ± 0.02 and AKBA with an R f estimation of 0.42 ± 0.02. The accuracy was seen to be as high as 99.17% and 97.42 for KBA and KBA, respectively. The percentage RSD value for intra-day and between day varieties was under 2%. The system indicated high affectability and specificity. Conclusion: The developed HPTLC method was simple, precise, robust, specific, rapid, and costeffective and could be used for quality control analysis and quantification of KBA and AKBA in different herbal formulations containing the plant species.

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http://hdl.handle.net/10453/140980