A Novel Approach in Crude Enzyme Laccase Production and Application in Emerging Contaminant Bioremediation

Nguyen, LN; Vu, MT; Johir, MAH; Pathak, N; Zdarta, J; Jesionowski, T; Semblante, GU; Hai, FI; Nguyen, HKD; Nghiem, LD

A Novel Approach in Crude Enzyme Laccase Production and Application in Emerging Contaminant Bioremediation

Nguyen, LN Vu, MT Johir, MAH Pathak, N Zdarta, J Jesionowski, T Semblante, GU Hai, FI Nguyen, HKD Nghiem, LD

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Processes, 8, (6), pp. 648-648

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Field	Value	Language
dc.contributor.author	Nguyen, LN
dc.contributor.author	Vu, MT
dc.contributor.author	Johir, MAH
dc.contributor.author	Pathak, N
dc.contributor.author	Zdarta, J
dc.contributor.author	Jesionowski, T
dc.contributor.author	Semblante, GU
dc.contributor.author	Hai, FI
dc.contributor.author	Nguyen, HKD
dc.contributor.author	Nghiem, LD
dc.date.accessioned	2020-06-08T01:34:30Z
dc.date.available	2020-06-08T01:34:30Z
dc.identifier.citation	Processes, 8, (6), pp. 648-648
dc.identifier.issn	2227-9717
dc.identifier.uri	http://hdl.handle.net/10453/141187
dc.description.abstract	<jats:p>Laccase enzyme from white-rot fungi is a potential biocatalyst for the oxidation of emerging contaminants (ECs), such as pesticides, pharmaceuticals and steroid hormones. This study aims to develop a three-step platform to treat ECs: (i) enzyme production, (ii) enzyme concentration and (iii) enzyme application. In the first step, solid culture and liquid culture were compared. The solid culture produced significantly more laccase than the liquid culture (447 vs. 74 µM/min after eight days), demonstrating that white rot fungi thrived on a solid medium. In the second step, the enzyme was concentrated 6.6 times using an ultrafiltration (UF) process, resulting in laccase activity of 2980 µM/min. No enzymatic loss due to filtration and membrane adsorption was observed, suggesting the feasibility of the UF membrane for enzyme concentration. In the third step, concentrated crude enzyme was applied in an enzymatic membrane reactor (EMR) to remove a diverse set of ECs (31 compounds in six groups). The EMR effectively removed of steroid hormones, phytoestrogen, ultraviolet (UV) filters and industrial chemical (above 90%). However, it had low removal of pesticides and pharmaceuticals.</jats:p>
dc.language	en
dc.publisher	MDPI AG
dc.relation.ispartof	Processes
dc.relation.isbasedon	10.3390/pr8060648
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0904 Chemical Engineering
dc.title	A Novel Approach in Crude Enzyme Laccase Production and Application in Emerging Contaminant Bioremediation
dc.type	Journal Article
utslib.citation.volume	8
utslib.for	0904 Chemical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-06-08T01:34:20Z
pubs.issue	6
pubs.publication-status	Published online
pubs.volume	8
utslib.start-page	648
utslib.citation.issue	6

Abstract:

Laccase enzyme from white-rot fungi is a potential biocatalyst for the oxidation of emerging contaminants (ECs), such as pesticides, pharmaceuticals and steroid hormones. This study aims to develop a three-step platform to treat ECs: (i) enzyme production, (ii) enzyme concentration and (iii) enzyme application. In the first step, solid culture and liquid culture were compared. The solid culture produced significantly more laccase than the liquid culture (447 vs. 74 µM/min after eight days), demonstrating that white rot fungi thrived on a solid medium. In the second step, the enzyme was concentrated 6.6 times using an ultrafiltration (UF) process, resulting in laccase activity of 2980 µM/min. No enzymatic loss due to filtration and membrane adsorption was observed, suggesting the feasibility of the UF membrane for enzyme concentration. In the third step, concentrated crude enzyme was applied in an enzymatic membrane reactor (EMR) to remove a diverse set of ECs (31 compounds in six groups). The EMR effectively removed of steroid hormones, phytoestrogen, ultraviolet (UV) filters and industrial chemical (above 90%). However, it had low removal of pesticides and pharmaceuticals.

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http://hdl.handle.net/10453/141187