Upconversion nanoparticles assisted multi-photon fluorescence saturation microscopy

Chen, C; Wang, F; Wen, S; Liu, Y; Shan, X; Jin, D

Upconversion nanoparticles assisted multi-photon fluorescence saturation microscopy

Chen, C

Wang, F

Wen, S

Liu, Y Shan, X Jin, D

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Publisher:: SPIE-INT SOC OPTICAL ENGINEERING
Publication Type:: Conference Proceeding
Citation:: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2019, 10891
Issue Date:: 2019-01-01

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Field	Value	Language
dc.contributor.author	Chen, C https://orcid.org/0000-0003-4620-7771
dc.contributor.author	Wang, F https://orcid.org/0000-0001-7403-3305
dc.contributor.author	Wen, S https://orcid.org/0000-0002-4670-4658
dc.contributor.author	Liu, Y
dc.contributor.author	Shan, X
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.editor	Nicolau, DV
dc.contributor.editor	Fixler, D
dc.contributor.editor	Goldys, EM
dc.date	2019-02-03
dc.date.accessioned	2020-06-10T22:15:04Z
dc.date.available	2020-06-10T22:15:04Z
dc.date.issued	2019-01-01
dc.identifier.citation	Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2019, 10891
dc.identifier.isbn	9781510624245
dc.identifier.issn	1605-7422
dc.identifier.issn	1996-756X
dc.identifier.uri	http://hdl.handle.net/10453/141232
dc.description.abstract	© 2019 SPIE. Bright and photo-stable luminescent nanoparticles held great potential for bioimaging, long-term molecular tracking. Rare-earth-doped upconversion nanoparticles (UCNPs) have been recently discovered with unique properties for Stimulated Emission Depletion (STED) super-resolution microscopy imaging. However, this system strictly requires optical alignment of concentric excitation and depletion beams, resulting in cost, stability, and complicity of the system. Taking the advantage of intermediate state saturation in UCNPs, emission saturation nanoscopy has been developed as a simplified modality by using a single doughnut excitation beam. In this work, we report that the emission saturation curve of fluorescence probes can modulate the performance of multi-photon emission saturation nanoscopy. With the precise synthesis of UCNPs, we demonstrate the resolution of this new imaging approach can be improved with five parameters, including emission band, activator doping, excitation power, sensitizer doping, core-shell. This approach opens a new strategy to a simple solution for super-resolution imaging and single molecule tracking at low cost, suggesting a large scope for materials science community to improve the performance of emission saturation nanoscopy.
dc.language	en
dc.publisher	SPIE-INT SOC OPTICAL ENGINEERING
dc.relation	http://purl.org/au-research/grants/arc/FT130100517
dc.relation.ispartof	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
dc.relation.ispartof	Conference on Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI
dc.relation.isbasedon	10.1117/12.2513733
dc.rights	info:eu-repo/semantics/closedAccess
dc.rights	Copyright 2019 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.	en_US
dc.title	Upconversion nanoparticles assisted multi-photon fluorescence saturation microscopy
dc.type	Conference Proceeding
utslib.citation.volume	10891
utslib.location.activity	San Francisco, CA
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Electrical and Data Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2020-06-10T22:14:48Z
pubs.finish-date	2019-02-04
pubs.publication-status	Published
pubs.start-date	2019-02-03
pubs.volume	10891

Abstract:

© 2019 SPIE. Bright and photo-stable luminescent nanoparticles held great potential for bioimaging, long-term molecular tracking. Rare-earth-doped upconversion nanoparticles (UCNPs) have been recently discovered with unique properties for Stimulated Emission Depletion (STED) super-resolution microscopy imaging. However, this system strictly requires optical alignment of concentric excitation and depletion beams, resulting in cost, stability, and complicity of the system. Taking the advantage of intermediate state saturation in UCNPs, emission saturation nanoscopy has been developed as a simplified modality by using a single doughnut excitation beam. In this work, we report that the emission saturation curve of fluorescence probes can modulate the performance of multi-photon emission saturation nanoscopy. With the precise synthesis of UCNPs, we demonstrate the resolution of this new imaging approach can be improved with five parameters, including emission band, activator doping, excitation power, sensitizer doping, core-shell. This approach opens a new strategy to a simple solution for super-resolution imaging and single molecule tracking at low cost, suggesting a large scope for materials science community to improve the performance of emission saturation nanoscopy.

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http://hdl.handle.net/10453/141232