Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review

Shanmugam, S; Ngo, HH; Wu, YR

Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review

Shanmugam, S Ngo, HH Wu, YR

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Renewable Energy, 2020, 149, pp. 1107-1119
Issue Date:: 2020

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Field	Value	Language
dc.contributor.author	Shanmugam, S
dc.contributor.author	Ngo, HH
dc.contributor.author	Wu, YR
dc.date.accessioned	2020-07-24T08:08:49Z
dc.date.available	2020-07-24T08:08:49Z
dc.date.issued	2020
dc.identifier.citation	Renewable Energy, 2020, 149, pp. 1107-1119
dc.identifier.issn	0960-1481
dc.identifier.issn	1879-0682
dc.identifier.uri	http://hdl.handle.net/10453/141861
dc.description.abstract	© 2019 Elsevier Ltd With rapid progress in the fields of synthetic biology and metabolic engineering, there are possible applications to generate a wide range of advanced biofuels with maximized yield and productivity to achieve a more sustainable bioprocess with reduced carbon footprints. Among the diverse molecular biology tools, clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) technology stands out with potential targeted genome editing, exhibiting a more precise and accurate gene knock-out and knock-in system better than its predecessors, for example zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN). There are reports involved in the advanced microbial genome engineering tools for the biofuels production; however, there is lack of a comprehensive review about the CRISPR-Cas based-techniques in improved biofuel production along with the strategies to reduce the off-target effect that ensures the success and safety of this method. Therefore, in this review we attempt to systematically comment on the mechanism of CRISPR-Cas and its application to microbial biofuels production. This includes bioethanol, biobutanol as well as other hydrocarbons that sequentially follow various suggestions on enhancing the efficiency of targeting genes. The role of inducible on/off genetic circuits in response to environmental stimuli in the regulation of targeted genome editing (TGE) by minimizing metabolic burden and maximizing fermentation efficiency is also discussed. The relevant stringent regulatory demands to ensure minimal off-target cleavage with maximum efficiency coupled with complete biosafety of this technology are considered. It can be concluded that the recent development of CRISPR-Cas technology should open a new avenue in creating microbial biorefineries for potentially enhanced biofuel production.
dc.language	en
dc.publisher	Elsevier BV
dc.relation.ispartof	Renewable Energy
dc.relation.isbasedon	10.1016/j.renene.2019.10.107
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject	0906 Electrical and Electronic Engineering, 0913 Mechanical Engineering, 0915 Interdisciplinary Engineering
dc.subject.classification	Energy
dc.title	Advanced CRISPR/Cas-based genome editing tools for microbial biofuels production: A review
dc.type	Journal Article
utslib.citation.volume	149
utslib.for	0906 Electrical and Electronic Engineering
utslib.for	0913 Mechanical Engineering
utslib.for	0915 Interdisciplinary Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
utslib.copyright.embargo	2022-01-01T00:00:00+1000Z
dc.date.updated	2020-07-24T08:08:44Z
pubs.publication-status	Published
pubs.volume	149

Abstract:

© 2019 Elsevier Ltd With rapid progress in the fields of synthetic biology and metabolic engineering, there are possible applications to generate a wide range of advanced biofuels with maximized yield and productivity to achieve a more sustainable bioprocess with reduced carbon footprints. Among the diverse molecular biology tools, clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) technology stands out with potential targeted genome editing, exhibiting a more precise and accurate gene knock-out and knock-in system better than its predecessors, for example zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN). There are reports involved in the advanced microbial genome engineering tools for the biofuels production; however, there is lack of a comprehensive review about the CRISPR-Cas based-techniques in improved biofuel production along with the strategies to reduce the off-target effect that ensures the success and safety of this method. Therefore, in this review we attempt to systematically comment on the mechanism of CRISPR-Cas and its application to microbial biofuels production. This includes bioethanol, biobutanol as well as other hydrocarbons that sequentially follow various suggestions on enhancing the efficiency of targeting genes. The role of inducible on/off genetic circuits in response to environmental stimuli in the regulation of targeted genome editing (TGE) by minimizing metabolic burden and maximizing fermentation efficiency is also discussed. The relevant stringent regulatory demands to ensure minimal off-target cleavage with maximum efficiency coupled with complete biosafety of this technology are considered. It can be concluded that the recent development of CRISPR-Cas technology should open a new avenue in creating microbial biorefineries for potentially enhanced biofuel production.

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http://hdl.handle.net/10453/141861