Upconversion Nonlinear Structured Illumination Microscopy.

Liu, B; Chen, C; Di, X; Liao, J; Wen, S; Su, QP; Shan, X; Xu, Z-Q; Ju, LA; Mi, C; Wang, F; Jin, D

Upconversion Nonlinear Structured Illumination Microscopy.

Liu, B Chen, C

Di, X Liao, J

Wen, S

Su, QP Shan, X Xu, Z-Q Ju, LA Mi, C Wang, F

Jin, D

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: Nano letters, 2020, 20, (7), pp. 4775-4781
Issue Date:: 2020-07

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Field	Value	Language
dc.contributor.author	Liu, B
dc.contributor.author	Chen, C https://orcid.org/0000-0003-4620-7771
dc.contributor.author	Di, X
dc.contributor.author	Liao, J https://orcid.org/0000-0003-0616-4762
dc.contributor.author	Wen, S https://orcid.org/0000-0002-4670-4658
dc.contributor.author	Su, QP
dc.contributor.author	Shan, X
dc.contributor.author	Xu, Z-Q
dc.contributor.author	Ju, LA
dc.contributor.author	Mi, C
dc.contributor.author	Wang, F https://orcid.org/0000-0001-7403-3305
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.date.accessioned	2020-10-16T03:25:24Z
dc.date.available	2020-10-16T03:25:24Z
dc.date.issued	2020-07
dc.identifier.citation	Nano letters, 2020, 20, (7), pp. 4775-4781
dc.identifier.issn	1530-6984
dc.identifier.issn	1530-6992
dc.identifier.uri	http://hdl.handle.net/10453/143294
dc.description.abstract	Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution observation of biological samples but is limited by the strong extinction of light by biological tissues, which restricts the imaging depth and degrades its imaging resolution. Here we report a photon upconversion scheme using lanthanide-doped nanoparticles for wide-field super-resolution imaging through the biological transparent window, featured by near-infrared and low-irradiance nonlinear structured illumination. We demonstrate that the 976 nm excitation and 800 nm upconverted emission can mitigate the aberration. We found that the nonlinear response of upconversion emissions from single nanoparticles can effectively generate the required high spatial frequency components in the Fourier domain. These strategies lead to a new modality in microscopy with a resolution below 131 nm, 1/7th of the excitation wavelength, and an imaging rate of 1 Hz.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation	http://purl.org/au-research/grants/arc/DP190101058
dc.relation	http://purl.org/au-research/grants/arc/DP200101970
dc.relation	http://purl.org/au-research/grants/arc/DE200100074
dc.relation.ispartof	Nano letters
dc.relation.isbasedon	10.1021/acs.nanolett.0c00448
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Nanoscience & Nanotechnology
dc.title	Upconversion Nonlinear Structured Illumination Microscopy.
dc.type	Journal Article
utslib.citation.volume	20
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Electrical and Data Engineering
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-10-16T03:25:13Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	20
utslib.citation.issue	7

Abstract:

Video-rate super-resolution imaging through biological tissue can visualize and track biomolecule interplays and transportations inside cellular organisms. Structured illumination microscopy allows for wide-field super resolution observation of biological samples but is limited by the strong extinction of light by biological tissues, which restricts the imaging depth and degrades its imaging resolution. Here we report a photon upconversion scheme using lanthanide-doped nanoparticles for wide-field super-resolution imaging through the biological transparent window, featured by near-infrared and low-irradiance nonlinear structured illumination. We demonstrate that the 976 nm excitation and 800 nm upconverted emission can mitigate the aberration. We found that the nonlinear response of upconversion emissions from single nanoparticles can effectively generate the required high spatial frequency components in the Fourier domain. These strategies lead to a new modality in microscopy with a resolution below 131 nm, 1/7th of the excitation wavelength, and an imaging rate of 1 Hz.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/143294