Ferrate effectively removes antibiotic resistance genes from wastewater through combined effect of microbial DNA damage and coagulation.

Ni, B-J; Yan, X; Dai, X; Liu, Z; Wei, W; Wu, S-L; Xu, Q; Sun, J

Ferrate effectively removes antibiotic resistance genes from wastewater through combined effect of microbial DNA damage and coagulation.

Ni, B-J Yan, X Dai, X Liu, Z Wei, W Wu, S-L Xu, Q Sun, J

Permalink

Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Water research, 2020, 185, pp. 116273
Issue Date:: 2020-10

Closed Access

	Filename	Description	Size
	1-s2.0-S0043135420308101-main.pdf	Published version	2.31 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Ni, B-J
dc.contributor.author	Yan, X
dc.contributor.author	Dai, X
dc.contributor.author	Liu, Z
dc.contributor.author	Wei, W
dc.contributor.author	Wu, S-L
dc.contributor.author	Xu, Q
dc.contributor.author	Sun, J
dc.date.accessioned	2021-01-07T02:24:22Z
dc.date.available	2020-08-04
dc.date.available	2021-01-07T02:24:22Z
dc.date.issued	2020-10
dc.identifier.citation	Water research, 2020, 185, pp. 116273
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/145150
dc.description.abstract	The widespread of antibiotic resistance genes (ARGs) in the environment can pose severe threats to public health. The wastewater treatment plant (WWTP) is regarded as an important hotspot of ARGs in the urban environment, but the removal of ARGs through conventional treatment techniques has been proven not sufficient. In this study, ferrate (Fe(VI)) was applied for the first time to remove intracellular ARGs from the secondary effluent of the WWTP. The results showed that Fe(VI) treatment could effectively remove 15 ARGs covering eight different types as well as intI1, the most common integron important to ARGs horizontal transfer. The removal efficiencies of tested genes could reach 1.10-4.37 log at the Fe(VI) dosage of 10 mg-Fe/L, which is significantly higher than those achieved through traditional disinfection methods. The DNA gel electrophoresis suggested that Fe(VI) could induce microbial DNA damage and consequently resulted in ARGs elimination. The presence of ARGs in settled residues indicated that coagulation initiated by Fe(VI) reduction products also contributed to ARGs removal from wastewater. In addition, the viability and relative abundances of potential ARGs hosts in the wastewater were decreased after Fe(VI) treatment. This study suggested a promising prospect for applying Fe(VI) to efficiently remove ARGs from wastewater, and consequently to control their proliferation and transfer in the environment.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartof	Water research
dc.relation.isbasedon	10.1016/j.watres.2020.116273
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	DNA Damage
dc.subject.mesh	Iron
dc.subject.mesh	DNA
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Genes, Bacterial
dc.subject.mesh	Waste Water
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	DNA
dc.subject.mesh	DNA Damage
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Genes, Bacterial
dc.subject.mesh	Iron
dc.subject.mesh	Waste Water
dc.title	Ferrate effectively removes antibiotic resistance genes from wastewater through combined effect of microbial DNA damage and coagulation.
dc.type	Journal Article
utslib.citation.volume	185
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-01-07T02:24:15Z
pubs.publication-status	Published
pubs.volume	185

Abstract:

The widespread of antibiotic resistance genes (ARGs) in the environment can pose severe threats to public health. The wastewater treatment plant (WWTP) is regarded as an important hotspot of ARGs in the urban environment, but the removal of ARGs through conventional treatment techniques has been proven not sufficient. In this study, ferrate (Fe(VI)) was applied for the first time to remove intracellular ARGs from the secondary effluent of the WWTP. The results showed that Fe(VI) treatment could effectively remove 15 ARGs covering eight different types as well as intI1, the most common integron important to ARGs horizontal transfer. The removal efficiencies of tested genes could reach 1.10-4.37 log at the Fe(VI) dosage of 10 mg-Fe/L, which is significantly higher than those achieved through traditional disinfection methods. The DNA gel electrophoresis suggested that Fe(VI) could induce microbial DNA damage and consequently resulted in ARGs elimination. The presence of ARGs in settled residues indicated that coagulation initiated by Fe(VI) reduction products also contributed to ARGs removal from wastewater. In addition, the viability and relative abundances of potential ARGs hosts in the wastewater were decreased after Fe(VI) treatment. This study suggested a promising prospect for applying Fe(VI) to efficiently remove ARGs from wastewater, and consequently to control their proliferation and transfer in the environment.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/145150