Impact of roxithromycin on waste activated sludge anaerobic digestion: Methane production, carbon transformation and antibiotic resistance genes.

Ni, B-J; Zeng, S; Wei, W; Dai, X; Sun, J

Impact of roxithromycin on waste activated sludge anaerobic digestion: Methane production, carbon transformation and antibiotic resistance genes.

Ni, B-J Zeng, S Wei, W Dai, X Sun, J

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: The Science of the total environment, 2020, 703, pp. 134899
Issue Date:: 2020-02

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Field	Value	Language
dc.contributor.author	Ni, B-J
dc.contributor.author	Zeng, S
dc.contributor.author	Wei, W
dc.contributor.author	Dai, X
dc.contributor.author	Sun, J
dc.date.accessioned	2021-02-10T06:41:34Z
dc.date.available	2019-10-07
dc.date.available	2021-02-10T06:41:34Z
dc.date.issued	2020-02
dc.identifier.citation	The Science of the total environment, 2020, 703, pp. 134899
dc.identifier.issn	0048-9697
dc.identifier.issn	1879-1026
dc.identifier.uri	http://hdl.handle.net/10453/146019
dc.description.abstract	The macrolide antibiotic roxithromycin is widely detected in varying aquatic environments, especially in the wastewater systems, as an emerging contaminant and leads to significant impacts on the microorganisms involved. In this study, the impact of a shock load of roxithromycin on waste activated sludge (WAS) anaerobic digestion was comprehensively investigated. The biochemical methane potential tests showed that the methane production from WAS anaerobic digestion was significantly inhibited by roxithromycin. With the dosage of roxithromycin increasing from 0 to 1000 μg/L, the maximum cumulative methane production decreased from 163.5 ± 2.6 mL/g VS to 150.9 ± 4.5 mL/g VS. In particular, roxithromycin inhibited the acidogenesis and methanogenesis in WAS anaerobic digestion, leading to the decreased methane production. The methanogenic archaea in the studied system mainly belonged to the genera of Methanoseata, Candidatus Methanofastidiosum and Methanolinea and their relative abundances also decreased with roxithromycin addition. The analysis of antibiotic resistance genes (ARGs) in the digested sludge indicated that the abundances of most ARGs detected in this study were increased with roxithromycin exposure, suggesting the potential of growing antibiotic resistance, which was probably caused by enhancing the effect of esterases, methylases and phosphorylases. This work reveals how roxithromycin affects the WAS anaerobic digestion and the change of ARGs in the anaerobic digestion with roxithromycin exposure, and provides useful information for practical operation.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER
dc.relation.ispartof	The Science of the total environment
dc.relation.isbasedon	10.1016/j.scitotenv.2019.134899
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Environmental Sciences
dc.subject.mesh	Carbon
dc.subject.mesh	Methane
dc.subject.mesh	Roxithromycin
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Bioreactors
dc.subject.mesh	Sewage
dc.subject.mesh	Waste Disposal, Fluid
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Bioreactors
dc.subject.mesh	Carbon
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Methane
dc.subject.mesh	Roxithromycin
dc.subject.mesh	Sewage
dc.subject.mesh	Waste Disposal, Fluid
dc.title	Impact of roxithromycin on waste activated sludge anaerobic digestion: Methane production, carbon transformation and antibiotic resistance genes.
dc.type	Journal Article
utslib.citation.volume	703
utslib.location.activity	Netherlands
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-02-10T06:41:19Z
pubs.publication-status	Published
pubs.volume	703

Abstract:

The macrolide antibiotic roxithromycin is widely detected in varying aquatic environments, especially in the wastewater systems, as an emerging contaminant and leads to significant impacts on the microorganisms involved. In this study, the impact of a shock load of roxithromycin on waste activated sludge (WAS) anaerobic digestion was comprehensively investigated. The biochemical methane potential tests showed that the methane production from WAS anaerobic digestion was significantly inhibited by roxithromycin. With the dosage of roxithromycin increasing from 0 to 1000 μg/L, the maximum cumulative methane production decreased from 163.5 ± 2.6 mL/g VS to 150.9 ± 4.5 mL/g VS. In particular, roxithromycin inhibited the acidogenesis and methanogenesis in WAS anaerobic digestion, leading to the decreased methane production. The methanogenic archaea in the studied system mainly belonged to the genera of Methanoseata, Candidatus Methanofastidiosum and Methanolinea and their relative abundances also decreased with roxithromycin addition. The analysis of antibiotic resistance genes (ARGs) in the digested sludge indicated that the abundances of most ARGs detected in this study were increased with roxithromycin exposure, suggesting the potential of growing antibiotic resistance, which was probably caused by enhancing the effect of esterases, methylases and phosphorylases. This work reveals how roxithromycin affects the WAS anaerobic digestion and the change of ARGs in the anaerobic digestion with roxithromycin exposure, and provides useful information for practical operation.

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http://hdl.handle.net/10453/146019