Domino P-µMB: A New Approach for the Sequential Immobilization of Enzymes Using Polydopamine/Polyethyleneimine Chemistry and Microfabrication

Changani, Z; Razmjou, A; Taheri-Kafrani, A; Asadnia, M

Domino P-µMB: A New Approach for the Sequential Immobilization of Enzymes Using Polydopamine/Polyethyleneimine Chemistry and Microfabrication

Changani, Z Razmjou, A Taheri-Kafrani, A Asadnia, M

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Advanced Materials Interfaces, 2020, 7, (13)
Issue Date:: 2020-07-01

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Field	Value	Language
dc.contributor.author	Changani, Z
dc.contributor.author	Razmjou, A
dc.contributor.author	Taheri-Kafrani, A
dc.contributor.author	Asadnia, M
dc.date.accessioned	2021-02-16T20:29:42Z
dc.date.available	2021-02-16T20:29:42Z
dc.date.issued	2020-07-01
dc.identifier.citation	Advanced Materials Interfaces, 2020, 7, (13)
dc.identifier.issn	2196-7350
dc.identifier.issn	2196-7350
dc.identifier.uri	http://hdl.handle.net/10453/146154
dc.description.abstract	© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Developing a facile approach for the manipulation of the direction and order of the enzymatic reactions via sequential immobilization on inexpensive substrates is a continuous demand. Herein, a new methodology is introduced that allows making a desired enzymatic reaction pathway on a paper-based microfluidic-membrane based biosensor (P-µMB). Although the method is universal, here, as a proof-of-concept, the sequential immobilization of α-amylase, glucose oxidase (GOx) and horseradish peroxidase (HRP) is presented for fabricating a P-µMB. To this end, hydrophilic polydopamine/polyethyleneimine patterns are created on the hydrophobic polypropylene membrane using 3D printing and a polydimethylsiloxane (PDMS) mold, and a coating layer of silver nanoparticles (AgNPs) is used to modify the patterns. The enzymes are then individually immobilized on the desired locations with another set of PDMS molds. It is observed that AgNPs P-µMB in the sequential immobilization system has stable activity at various temperature and pH regimes, high selectivity toward starch, wide-range linear sensitivity, and a limit of detection of 0.002% w/w starch. A smartphone camera is used for the quantitative analysis of the analyte with the mean gray intensity as the analytical parameter. This developed system provides a platform for further sequential immobilization of other types of biological elements.
dc.language	English
dc.publisher	WILEY
dc.relation.ispartof	Advanced Materials Interfaces
dc.relation.isbasedon	10.1002/admi.201901864
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0306 Physical Chemistry (incl. Structural), 0912 Materials Engineering
dc.title	Domino P-µMB: A New Approach for the Sequential Immobilization of Enzymes Using Polydopamine/Polyethyleneimine Chemistry and Microfabrication
dc.type	Journal Article
utslib.citation.volume	7
utslib.for	0306 Physical Chemistry (incl. Structural)
utslib.for	0912 Materials Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-02-16T20:29:07Z
pubs.issue	13
pubs.publication-status	Published
pubs.volume	7
utslib.citation.issue	13

Abstract:

© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Developing a facile approach for the manipulation of the direction and order of the enzymatic reactions via sequential immobilization on inexpensive substrates is a continuous demand. Herein, a new methodology is introduced that allows making a desired enzymatic reaction pathway on a paper-based microfluidic-membrane based biosensor (P-µMB). Although the method is universal, here, as a proof-of-concept, the sequential immobilization of α-amylase, glucose oxidase (GOx) and horseradish peroxidase (HRP) is presented for fabricating a P-µMB. To this end, hydrophilic polydopamine/polyethyleneimine patterns are created on the hydrophobic polypropylene membrane using 3D printing and a polydimethylsiloxane (PDMS) mold, and a coating layer of silver nanoparticles (AgNPs) is used to modify the patterns. The enzymes are then individually immobilized on the desired locations with another set of PDMS molds. It is observed that AgNPs P-µMB in the sequential immobilization system has stable activity at various temperature and pH regimes, high selectivity toward starch, wide-range linear sensitivity, and a limit of detection of 0.002% w/w starch. A smartphone camera is used for the quantitative analysis of the analyte with the mean gray intensity as the analytical parameter. This developed system provides a platform for further sequential immobilization of other types of biological elements.

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http://hdl.handle.net/10453/146154