Enzymatic biodiesel production from crude Eruca sativa oil using Candida rugosa lipase in a solvent-free system using response surface methodology

Aghababaie, M; Beheshti, M; Razmjou, A; Bordbar, AK

Enzymatic biodiesel production from crude Eruca sativa oil using Candida rugosa lipase in a solvent-free system using response surface methodology

Aghababaie, M Beheshti, M Razmjou, A Bordbar, AK

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Publisher:: TAYLOR & FRANCIS LTD
Publication Type:: Journal Article
Citation:: Biofuels, 2020, 11, (1), pp. 93-99
Issue Date:: 2020-01-02

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Field	Value	Language
dc.contributor.author	Aghababaie, M
dc.contributor.author	Beheshti, M
dc.contributor.author	Razmjou, A
dc.contributor.author	Bordbar, AK
dc.date.accessioned	2021-02-16T22:59:55Z
dc.date.available	2021-02-16T22:59:55Z
dc.date.issued	2020-01-02
dc.identifier.citation	Biofuels, 2020, 11, (1), pp. 93-99
dc.identifier.issn	1759-7269
dc.identifier.issn	1759-7277
dc.identifier.uri	http://hdl.handle.net/10453/146158
dc.description.abstract	© 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group. In the present study, enzymatic biodiesel production from low-cost, non-edible crude Eruca sativa oil was investigated. Candida rugosa lipase (CRL), a commercially promising biocatalyst in different industries, was used. Although this source of lipase has been reported as unsuitable for biodiesel production from non-edible oil, in this study, it was attempted to use this enzyme for production of fatty acid methyl esters (FAME) from Eruca sativa oil in a solvent-free system. For the first time, the effects of pre-hydrolysis and pH on the yield of biodiesel were evaluated. Afterward, the four most commonly reported influential parameters were analyzed with the use of response surface methodology (RSM). It was observed that pre-hydrolysis had a significant effect on biodiesel conversion due to the mechanism of enzymatic biodiesel production. Deionized water was more efficient in comparison with phosphate buffer with different pH values. According to the RSM analysis, water content and temperature have a highly significant effect on biodiesel production. Methanol-to-oil ratio and enzyme amounts were also significant factors in FAME production. The optimum methanol-to-oil ratio, enzyme amount, water content, and temperature for achieving high conversion was found to be 3:1, 5 mg, 40%, and 21 °C, respectively.
dc.language	English
dc.publisher	TAYLOR & FRANCIS LTD
dc.relation.ispartof	Biofuels
dc.relation.isbasedon	10.1080/17597269.2017.1345359
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0607 Plant Biology, 1003 Industrial Biotechnology
dc.title	Enzymatic biodiesel production from crude Eruca sativa oil using Candida rugosa lipase in a solvent-free system using response surface methodology
dc.type	Journal Article
utslib.citation.volume	11
utslib.for	090405 Non-automotive Combustion and Fuel Engineering (incl. Alternative/Renewable Fuels)
utslib.for	1003 Industrial Biotechnology
utslib.for	0607 Plant Biology
utslib.for	1003 Industrial Biotechnology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-02-16T22:59:51Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	11
utslib.citation.issue	1

Abstract:

© 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group. In the present study, enzymatic biodiesel production from low-cost, non-edible crude Eruca sativa oil was investigated. Candida rugosa lipase (CRL), a commercially promising biocatalyst in different industries, was used. Although this source of lipase has been reported as unsuitable for biodiesel production from non-edible oil, in this study, it was attempted to use this enzyme for production of fatty acid methyl esters (FAME) from Eruca sativa oil in a solvent-free system. For the first time, the effects of pre-hydrolysis and pH on the yield of biodiesel were evaluated. Afterward, the four most commonly reported influential parameters were analyzed with the use of response surface methodology (RSM). It was observed that pre-hydrolysis had a significant effect on biodiesel conversion due to the mechanism of enzymatic biodiesel production. Deionized water was more efficient in comparison with phosphate buffer with different pH values. According to the RSM analysis, water content and temperature have a highly significant effect on biodiesel production. Methanol-to-oil ratio and enzyme amounts were also significant factors in FAME production. The optimum methanol-to-oil ratio, enzyme amount, water content, and temperature for achieving high conversion was found to be 3:1, 5 mg, 40%, and 21 °C, respectively.

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http://hdl.handle.net/10453/146158