Insights into the microbial response of anaerobic granular sludge during long-term exposure to polyethylene terephthalate microplastics.

Zhang, Y-T; Wei, W; Huang, Q-S; Wang, C; Wang, Y; Ni, B-J

Insights into the microbial response of anaerobic granular sludge during long-term exposure to polyethylene terephthalate microplastics.

Zhang, Y-T Wei, W Huang, Q-S Wang, C Wang, Y Ni, B-J

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Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Water research, 2020, 179, pp. 115898
Issue Date:: 2020-07

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Field	Value	Language
dc.contributor.author	Zhang, Y-T
dc.contributor.author	Wei, W
dc.contributor.author	Huang, Q-S
dc.contributor.author	Wang, C
dc.contributor.author	Wang, Y
dc.contributor.author	Ni, B-J
dc.date.accessioned	2021-02-19T22:07:46Z
dc.date.available	2020-04-27
dc.date.available	2021-02-19T22:07:46Z
dc.date.issued	2020-07
dc.identifier.citation	Water research, 2020, 179, pp. 115898
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/146230
dc.description.abstract	The negative effects of ubiquitous microplastics on wastewater treatment have attracted increasing attention. However, the potential impacts of microplastics on anaerobic granular sludge (AGS) remain unknown. To fill this knowledge gap, this paper investigated the response of AGS to the exposure of model microplastics (polyethylene terephthalate (PET-MPs)) and provided insights into the mechanisms involved. The 84 days' long-term exposure experiments demonstrated that PET-MPs, at relatively low level (15 MP L-1) did not affect AGS performance during anaerobic wastewater treatment, while 75-300 MP L-1 of PET-MPs caused the decreases of COD removal efficiency and methane yields by 17.4-30.4% and 17.2-28.4%, accompanied with the 119.4-227.8% increase in short-chain fatty acid (SCFA) accumulation and particle breakage. Extracellular polymeric substances (EPS) analysis showed that dosage-dependent tolerance of AGS to PET-MPs was attributed to the induced EPS producing protection role, but PET-MPs at higher concentrations (75-300 MP L-1) suppressed EPS generation. Correspondingly, microbial community analysis revealed that the populations of key acidogens (e.g., Levilinea sp.) and methanogens (e.g., Methanosaeta sp.) decreased after long-term exposure to PET-MPs. Assessment of the toxicity of PET-MPs revealed that the leached di-n-butyl phthalate (DBP) and the induced reactive oxygen species (ROS) by PET-MPs were causing toxicity towards AGS, confirmed by the increases in cell mortality and lactate dehydrogenase (LDH) release. These results provide novel insights into the ecological risk assessment of microplastics in anaerobic wastewater treatment system.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartof	Water research
dc.relation.isbasedon	10.1016/j.watres.2020.115898
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	Plastics
dc.subject.mesh	Polyethylene Terephthalates
dc.subject.mesh	Sewage
dc.subject.mesh	Waste Disposal, Fluid
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Microplastics
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Microplastics
dc.subject.mesh	Plastics
dc.subject.mesh	Polyethylene Terephthalates
dc.subject.mesh	Sewage
dc.subject.mesh	Waste Disposal, Fluid
dc.title	Insights into the microbial response of anaerobic granular sludge during long-term exposure to polyethylene terephthalate microplastics.
dc.type	Journal Article
utslib.citation.volume	179
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-02-19T22:07:29Z
pubs.publication-status	Published
pubs.volume	179

Abstract:

The negative effects of ubiquitous microplastics on wastewater treatment have attracted increasing attention. However, the potential impacts of microplastics on anaerobic granular sludge (AGS) remain unknown. To fill this knowledge gap, this paper investigated the response of AGS to the exposure of model microplastics (polyethylene terephthalate (PET-MPs)) and provided insights into the mechanisms involved. The 84 days' long-term exposure experiments demonstrated that PET-MPs, at relatively low level (15 MP L-1) did not affect AGS performance during anaerobic wastewater treatment, while 75-300 MP L-1 of PET-MPs caused the decreases of COD removal efficiency and methane yields by 17.4-30.4% and 17.2-28.4%, accompanied with the 119.4-227.8% increase in short-chain fatty acid (SCFA) accumulation and particle breakage. Extracellular polymeric substances (EPS) analysis showed that dosage-dependent tolerance of AGS to PET-MPs was attributed to the induced EPS producing protection role, but PET-MPs at higher concentrations (75-300 MP L-1) suppressed EPS generation. Correspondingly, microbial community analysis revealed that the populations of key acidogens (e.g., Levilinea sp.) and methanogens (e.g., Methanosaeta sp.) decreased after long-term exposure to PET-MPs. Assessment of the toxicity of PET-MPs revealed that the leached di-n-butyl phthalate (DBP) and the induced reactive oxygen species (ROS) by PET-MPs were causing toxicity towards AGS, confirmed by the increases in cell mortality and lactate dehydrogenase (LDH) release. These results provide novel insights into the ecological risk assessment of microplastics in anaerobic wastewater treatment system.

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http://hdl.handle.net/10453/146230