MicroRNA expression profiles in head and neck cancer cell lines

Tran, N; McLean, T; Zhang, X; Zhao, CJ; Thomson, JM; O'Brien, C; Rose, B

MicroRNA expression profiles in head and neck cancer cell lines

Tran, N

McLean, T Zhang, X Zhao, CJ Thomson, JM O'Brien, C Rose, B

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Publication Type:: Journal Article
Citation:: Biochemical and Biophysical Research Communications, 2007, 358 (1), pp. 12 - 17
Issue Date:: 2007-06-22

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Field	Value	Language
dc.contributor.author	Tran, N https://orcid.org/0000-0001-7747-2530	en_US
dc.contributor.author	McLean, T	en_US
dc.contributor.author	Zhang, X	en_US
dc.contributor.author	Zhao, CJ	en_US
dc.contributor.author	Thomson, JM	en_US
dc.contributor.author	O'Brien, C	en_US
dc.contributor.author	Rose, B	en_US
dc.date.available	2007-03-20	en_US
dc.date.issued	2007-06-22	en_US
dc.identifier.citation	Biochemical and Biophysical Research Communications, 2007, 358 (1), pp. 12 - 17	en_US
dc.identifier.issn	0006-291X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14626
dc.description.abstract	Non-coding RNA molecules such as microRNAs (miRNAs) may play an important role in human carcinogenesis. Their expression has been profiled in many human cancers but there are few published studies in head and neck cancer. In this study, the relative expression of 261 mature miRNA genes was determined in nine head and neck cancer cell lines using an oligonucleotide array platform. Thirty-three miRNAs in the array were found to be highly expressed and 22 showed low levels of expression in all cell lines. Notable was the high expression of miR-21 and miR-205. Expression of several miRNAs was validated using Northern blot analysis. Potential targets of validated miRNAs included tumor suppressor genes, kinesin family member 1B isoform alpha (KIF1B), and hypermethylated in cancer 2 (HIC2), and pleomorphic adenoma gene 1 (PLAG1). This study provides the largest genomewide survey of mature miRNA transcripts in head and neck cancer cell lines. Crown Copyright © 2007.	en_US
dc.relation.ispartof	Biochemical and Biophysical Research Communications	en_US
dc.relation.isbasedon	10.1016/j.bbrc.2007.03.201	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Head and Neck Neoplasms	en_US
dc.subject.mesh	Kinesin	en_US
dc.subject.mesh	DNA-Binding Proteins	en_US
dc.subject.mesh	Nerve Tissue Proteins	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Biomarkers, Tumor	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Gene Expression Regulation, Neoplastic	en_US
dc.subject.mesh	Oligonucleotide Array Sequence Analysis	en_US
dc.title	MicroRNA expression profiles in head and neck cancer cell lines	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	358	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
dc.location.activity	ISI:000246790800003	en_US
dc.location.activity	Sydney
dc.location.activity	United States
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	358	en_US

Abstract:

Non-coding RNA molecules such as microRNAs (miRNAs) may play an important role in human carcinogenesis. Their expression has been profiled in many human cancers but there are few published studies in head and neck cancer. In this study, the relative expression of 261 mature miRNA genes was determined in nine head and neck cancer cell lines using an oligonucleotide array platform. Thirty-three miRNAs in the array were found to be highly expressed and 22 showed low levels of expression in all cell lines. Notable was the high expression of miR-21 and miR-205. Expression of several miRNAs was validated using Northern blot analysis. Potential targets of validated miRNAs included tumor suppressor genes, kinesin family member 1B isoform alpha (KIF1B), and hypermethylated in cancer 2 (HIC2), and pleomorphic adenoma gene 1 (PLAG1). This study provides the largest genomewide survey of mature miRNA transcripts in head and neck cancer cell lines. Crown Copyright © 2007.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/14626