Medium-Chain fatty acids and long-chain alcohols production from waste activated sludge via two-stage anaerobic fermentation.

Wu, S-L; Wei, W; Sun, J; Xu, Q; Dai, X; Ni, B-J

Medium-Chain fatty acids and long-chain alcohols production from waste activated sludge via two-stage anaerobic fermentation.

Wu, S-L Wei, W Sun, J Xu, Q Dai, X Ni, B-J

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Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Water research, 2020, 186, pp. 116381
Issue Date:: 2020-11

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Field	Value	Language
dc.contributor.author	Wu, S-L
dc.contributor.author	Wei, W
dc.contributor.author	Sun, J
dc.contributor.author	Xu, Q
dc.contributor.author	Dai, X
dc.contributor.author	Ni, B-J
dc.date.accessioned	2021-03-01T00:29:10Z
dc.date.available	2020-09-01
dc.date.available	2021-03-01T00:29:10Z
dc.date.issued	2020-11
dc.identifier.citation	Water research, 2020, 186, pp. 116381
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/146540
dc.description.abstract	Traditional bioenergy recovery in the form of short chain fatty acids (SCFAs) from waste activated sludge (WAS) is generally limited by economic unattractiveness and complexity of products separation. Herein, a novel biotechnology process of two-stage anaerobic fermentation for converting the WAS into high energy density, easy-separated medium chain fatty acids (MCFAs) and long-chain alcohols (LCAs) was evaluated. In this process, the WAS was first converted to WAS alkaline fermentation liquid (WASAFL), serving as electron acceptors (EAs) and inoculum, then adding ethanol as electron donor (ED) for chain elongation (CE). The co-production of MCFAs and LCAs during CE were studied under three different ED to EA ratios, i.e., 3:1, 4:1 and 5:1. Experimental results demonstrated that when the ratio of ED to EA increased from 3:1 to 5:1, the production of MCFA and LCAs respectively increased from 5.57 ± 0.17 and 2.58 ± 0.18 to7.67 ± 0.48 and 4.21 ± 0.19 g COD/L. A similar observation was made in the total product electron efficiency, increasing from 59.9% to 72.1%. However, the highest total product selectivity (i.e., 68.0%) and highest products production yield (i.e., 59.77%) were not achieved at the ED to EA ratio of 5:1 due to toxicity caused by higher accumulation of n-caproate. The kinetic analysis further confirmed that high ratio of ED to EA induced improvement in product maximum yield, production rate for both MCFAs and LCAs. Microbial community analysis indicated that Clostridium, Caproiciproducens, Acinetobacter, Exilispira, and Oscillibacter were clearly enriched in the CE reactor and had positive correlation with MCFAs and LCAs production.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartof	Water research
dc.relation.isbasedon	10.1016/j.watres.2020.116381
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	Fatty Acids
dc.subject.mesh	Fatty Acids, Volatile
dc.subject.mesh	Sewage
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Fermentation
dc.subject.mesh	Kinetics
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Fatty Acids
dc.subject.mesh	Fatty Acids, Volatile
dc.subject.mesh	Fermentation
dc.subject.mesh	Kinetics
dc.subject.mesh	Sewage
dc.title	Medium-Chain fatty acids and long-chain alcohols production from waste activated sludge via two-stage anaerobic fermentation.
dc.type	Journal Article
utslib.citation.volume	186
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-03-01T00:29:08Z
pubs.publication-status	Published
pubs.volume	186

Abstract:

Traditional bioenergy recovery in the form of short chain fatty acids (SCFAs) from waste activated sludge (WAS) is generally limited by economic unattractiveness and complexity of products separation. Herein, a novel biotechnology process of two-stage anaerobic fermentation for converting the WAS into high energy density, easy-separated medium chain fatty acids (MCFAs) and long-chain alcohols (LCAs) was evaluated. In this process, the WAS was first converted to WAS alkaline fermentation liquid (WASAFL), serving as electron acceptors (EAs) and inoculum, then adding ethanol as electron donor (ED) for chain elongation (CE). The co-production of MCFAs and LCAs during CE were studied under three different ED to EA ratios, i.e., 3:1, 4:1 and 5:1. Experimental results demonstrated that when the ratio of ED to EA increased from 3:1 to 5:1, the production of MCFA and LCAs respectively increased from 5.57 ± 0.17 and 2.58 ± 0.18 to7.67 ± 0.48 and 4.21 ± 0.19 g COD/L. A similar observation was made in the total product electron efficiency, increasing from 59.9% to 72.1%. However, the highest total product selectivity (i.e., 68.0%) and highest products production yield (i.e., 59.77%) were not achieved at the ED to EA ratio of 5:1 due to toxicity caused by higher accumulation of n-caproate. The kinetic analysis further confirmed that high ratio of ED to EA induced improvement in product maximum yield, production rate for both MCFAs and LCAs. Microbial community analysis indicated that Clostridium, Caproiciproducens, Acinetobacter, Exilispira, and Oscillibacter were clearly enriched in the CE reactor and had positive correlation with MCFAs and LCAs production.

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http://hdl.handle.net/10453/146540