Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation.

Mehta, M; Paudel, KR; Shukla, SD; Shastri, MD; Satija, S; Singh, SK; Gulati, M; Dureja, H; Zacconi, FC; Hansbro, PM; Chellappan, DK; Dua, K

Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation.

Mehta, M Paudel, KR Shukla, SD Shastri, MD Satija, S

Singh, SK Gulati, M Dureja, H Zacconi, FC Hansbro, PM Chellappan, DK Dua, K

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Publisher:: Future Science Ltd
Publication Type:: Journal Article
Citation:: Future medicinal chemistry, 2021, 0, (0)
Issue Date:: 2021-02-04

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Field	Value	Language
dc.contributor.author	Mehta, M
dc.contributor.author	Paudel, KR
dc.contributor.author	Shukla, SD
dc.contributor.author	Shastri, MD
dc.contributor.author	Satija, S https://orcid.org/0000-0003-4702-6534
dc.contributor.author	Singh, SK
dc.contributor.author	Gulati, M
dc.contributor.author	Dureja, H
dc.contributor.author	Zacconi, FC
dc.contributor.author	Hansbro, PM
dc.contributor.author	Chellappan, DK
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.date.accessioned	2021-03-03T04:07:04Z
dc.date.available	2021-03-03T04:07:04Z
dc.date.issued	2021-02-04
dc.identifier.citation	Future medicinal chemistry, 2021, 0, (0)
dc.identifier.issn	1756-8919
dc.identifier.issn	1756-8927
dc.identifier.uri	http://hdl.handle.net/10453/146696
dc.description.abstract	<b>Aim:</b> In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (<i>NQO1</i>); γ-glutamyl cysteine synthetase catalytic subunit (<i>GCLC</i>)) and pro-oxidant (NADPH oxidase <i>(Nox)-4</i>; <i>Nox2B</i>) genes. <b>Results:</b> Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely <i>Nox2B</i> and <i>Nox4</i>, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes <i>Gclc</i> and <i>Nqo-1</i> in a dose-dependent manner. <b>Conclusion:</b> The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Future Science Ltd
dc.relation.ispartof	Future medicinal chemistry
dc.relation.isbasedon	10.4155/fmc-2020-0297
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0304 Medicinal and Biomolecular Chemistry, 1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Medicinal & Biomolecular Chemistry
dc.title	Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation.
dc.type	Journal Article
utslib.citation.volume	0
utslib.location.activity	England
utslib.for	0304 Medicinal and Biomolecular Chemistry
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2021-03-03T04:07:02Z
pubs.issue	0
pubs.publication-status	Published
pubs.volume	0
utslib.citation.issue	0

Abstract:

Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); γ-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/146696