Modeling of Polyhydroxyalkanoate Synthesis from Biogas by Methylocystis hirsuta

Chen, X; Rodríguez, Y; López, JC; Muñoz, R; Ni, BJ; Sin, G

Modeling of Polyhydroxyalkanoate Synthesis from Biogas by Methylocystis hirsuta

Chen, X Rodríguez, Y López, JC Muñoz, R Ni, BJ Sin, G

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: ACS Sustainable Chemistry and Engineering, 2020, 8, (9), pp. 3906-3912
Issue Date:: 2020-03-09

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Field	Value	Language
dc.contributor.author	Chen, X
dc.contributor.author	Rodríguez, Y
dc.contributor.author	López, JC
dc.contributor.author	Muñoz, R
dc.contributor.author	Ni, BJ
dc.contributor.author	Sin, G
dc.date.accessioned	2021-03-07T05:32:56Z
dc.date.available	2021-03-07T05:32:56Z
dc.date.issued	2020-03-09
dc.identifier.citation	ACS Sustainable Chemistry and Engineering, 2020, 8, (9), pp. 3906-3912
dc.identifier.issn	2168-0485
dc.identifier.issn	2168-0485
dc.identifier.uri	http://hdl.handle.net/10453/146847
dc.description.abstract	Copyright © 2020 American Chemical Society. Methylocystis hirsuta, a type II methanotroph, has been experimentally demonstrated to be able to efficiently synthesize polyhydroxyalkanoates (PHA) from biogas under nutrient-limited conditions. A mechanistic model capable of describing the relevant processes of M. hirsuta, which is currently not available, would therefore lay a solid foundation for future practical demonstration and optimization of the PHA synthesis technology using biogas. To this end, dedicated batch tests were designed and conducted to obtain experimental data for different mechanistic processes of M. hirsuta. Through utilizing the experimental data of well-designed batch tests and following a step-wise model calibration/validation protocol, the stoichiometrics and kinetics of M. hirsuta are reported for the first time, including the yields of growth and PHA synthesis on CH4 (0.14 ± 0.01 g COD g-1 COD and 0.25 ± 0.02 g COD g-1 COD), the CH4 and O2 affinity constants (5.1 ± 2.1 g COD m-3 and 4.1 ± 1.7 g O2 m-3), the maximum PHA consumption rate (0.019 ± 0.001 g COD g-1 COD d-1), and the maximum PHA synthesis rate on CH4 (0.39 ± 0.05 g COD g-1 COD d-1). Through applying the developed model, an optimal O2:CH4 molar ratio of 1.6 mol O2 mol-1 CH4 was found to maximize the PHA synthesis by M. hirsuta. Practically, the model and parameters obtained would not only benefit the design and operation of bioreactors performing PHA synthesis from biogas, but also enable specific research on selection for type II methanotrophs in diverse environments.
dc.language	English
dc.publisher	AMER CHEMICAL SOC
dc.relation	http://purl.org/au-research/grants/arc/FT160100195
dc.relation.ispartof	ACS Sustainable Chemistry and Engineering
dc.relation.isbasedon	10.1021/acssuschemeng.9b07414
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0502 Environmental Science and Management, 0904 Chemical Engineering
dc.title	Modeling of Polyhydroxyalkanoate Synthesis from Biogas by Methylocystis hirsuta
dc.type	Journal Article
utslib.citation.volume	8
utslib.for	0301 Analytical Chemistry
utslib.for	0502 Environmental Science and Management
utslib.for	0904 Chemical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-03-07T05:32:55Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	8
utslib.citation.issue	9

Abstract:

Copyright © 2020 American Chemical Society. Methylocystis hirsuta, a type II methanotroph, has been experimentally demonstrated to be able to efficiently synthesize polyhydroxyalkanoates (PHA) from biogas under nutrient-limited conditions. A mechanistic model capable of describing the relevant processes of M. hirsuta, which is currently not available, would therefore lay a solid foundation for future practical demonstration and optimization of the PHA synthesis technology using biogas. To this end, dedicated batch tests were designed and conducted to obtain experimental data for different mechanistic processes of M. hirsuta. Through utilizing the experimental data of well-designed batch tests and following a step-wise model calibration/validation protocol, the stoichiometrics and kinetics of M. hirsuta are reported for the first time, including the yields of growth and PHA synthesis on CH4 (0.14 ± 0.01 g COD g-1 COD and 0.25 ± 0.02 g COD g-1 COD), the CH4 and O2 affinity constants (5.1 ± 2.1 g COD m-3 and 4.1 ± 1.7 g O2 m-3), the maximum PHA consumption rate (0.019 ± 0.001 g COD g-1 COD d-1), and the maximum PHA synthesis rate on CH4 (0.39 ± 0.05 g COD g-1 COD d-1). Through applying the developed model, an optimal O2:CH4 molar ratio of 1.6 mol O2 mol-1 CH4 was found to maximize the PHA synthesis by M. hirsuta. Practically, the model and parameters obtained would not only benefit the design and operation of bioreactors performing PHA synthesis from biogas, but also enable specific research on selection for type II methanotrophs in diverse environments.

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http://hdl.handle.net/10453/146847