Mussel inspired ZIF8 microcarriers: a new approach for large-scale production of stem cells

Asadniaye Fardjahromi, M; Razmjou, A; Vesey, G; Ejeian, F; Banerjee, B; Chandra Mukhopadhyay, S; Ebrahimi Warkiani, M

Mussel inspired ZIF8 microcarriers: a new approach for large-scale production of stem cells

Asadniaye Fardjahromi, M Razmjou, A Vesey, G Ejeian, F Banerjee, B Chandra Mukhopadhyay, S Ebrahimi Warkiani, M

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: RSC Advances, 2020, 10, (34), pp. 20118-20128
Issue Date:: 2020-05-27

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Field	Value	Language
dc.contributor.author	Asadniaye Fardjahromi, M
dc.contributor.author	Razmjou, A
dc.contributor.author	Vesey, G
dc.contributor.author	Ejeian, F
dc.contributor.author	Banerjee, B
dc.contributor.author	Chandra Mukhopadhyay, S
dc.contributor.author	Ebrahimi Warkiani, M https://orcid.org/0000-0002-4184-1944
dc.date.accessioned	2021-03-14T06:23:22Z
dc.date.available	2021-03-14T06:23:22Z
dc.date.issued	2020-05-27
dc.identifier.citation	RSC Advances, 2020, 10, (34), pp. 20118-20128
dc.identifier.issn	2046-2069
dc.identifier.issn	2046-2069
dc.identifier.uri	http://hdl.handle.net/10453/147104
dc.description.abstract	© The Royal Society of Chemistry 2020. Metal-organic frameworks (MOFs) have high porosity, large surface area, and tunable functionality and have been widely used for drug loading. The aim of this study was to exploit unique features of zeolitic imidazolate framework-8 (ZIF8) to develop an innovative composite microcarrier (MC) for human mesenchymal stem cells (hMSCs) adhesion and proliferation. ZIF8 MCs were prepared by immobilization of polydopamine/polyethyleneimine (PDA/PEI) and ZIF8 on the surface of polystyrene beads. The chemical properties of MCs such as coating stability and homogeneity were characterized by different techniques such as ATR-FTIR, XRD, EDX, SEM, and contact angle. The prepared MCs were tested using human adipose-derived mesenchymal stem cells (hADSCs) in both static and dynamic conditions, and results were compared to a commercially available MC (Star-Plus), polydopamine coated MCs and amine-functionalized MCs as a control. Results show that PDA/PEI/ZIF8 coated MCs (in short: ZIF8 MCs) provides an excellent biocompatible environment for hADSCs adhesion and growth. In conclusion, ZIF8 MCs represent suitable and low-cost support for hADSCs culture and expansion, which can maximize cell yield and viability while preserving hADSCs multipotency. The present findings have revealed this strategy has the potential for chemical and topographical modification of MCs in tissue engineering applications.
dc.language	English
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation	http://purl.org/au-research/grants/arc/DP170103704
dc.relation	http://purl.org/au-research/grants/arc/DP180103003
dc.relation	http://purl.org/au-research/grants/nhmrc/GNT1143377
dc.relation	http://purl.org/au-research/grants/nhmrc/1143377
dc.relation.ispartof	RSC Advances
dc.relation.isbasedon	10.1039/d0ra04090h
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	03 Chemical Sciences
dc.title	Mussel inspired ZIF8 microcarriers: a new approach for large-scale production of stem cells
dc.type	Journal Article
utslib.citation.volume	10
utslib.for	03 Chemical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access	*
dc.date.updated	2021-03-14T06:23:20Z
pubs.issue	34
pubs.publication-status	Published
pubs.volume	10
utslib.citation.issue	34

Abstract:

© The Royal Society of Chemistry 2020. Metal-organic frameworks (MOFs) have high porosity, large surface area, and tunable functionality and have been widely used for drug loading. The aim of this study was to exploit unique features of zeolitic imidazolate framework-8 (ZIF8) to develop an innovative composite microcarrier (MC) for human mesenchymal stem cells (hMSCs) adhesion and proliferation. ZIF8 MCs were prepared by immobilization of polydopamine/polyethyleneimine (PDA/PEI) and ZIF8 on the surface of polystyrene beads. The chemical properties of MCs such as coating stability and homogeneity were characterized by different techniques such as ATR-FTIR, XRD, EDX, SEM, and contact angle. The prepared MCs were tested using human adipose-derived mesenchymal stem cells (hADSCs) in both static and dynamic conditions, and results were compared to a commercially available MC (Star-Plus), polydopamine coated MCs and amine-functionalized MCs as a control. Results show that PDA/PEI/ZIF8 coated MCs (in short: ZIF8 MCs) provides an excellent biocompatible environment for hADSCs adhesion and growth. In conclusion, ZIF8 MCs represent suitable and low-cost support for hADSCs culture and expansion, which can maximize cell yield and viability while preserving hADSCs multipotency. The present findings have revealed this strategy has the potential for chemical and topographical modification of MCs in tissue engineering applications.

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http://hdl.handle.net/10453/147104