Polystyrene nanoplastics reshape the anaerobic granular sludge for recovering methane from wastewater.

Wei, W; Hao, Q; Chen, Z; Bao, T; Ni, B-J

Polystyrene nanoplastics reshape the anaerobic granular sludge for recovering methane from wastewater.

Wei, W Hao, Q

Chen, Z

Bao, T Ni, B-J

Permalink

Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Water research, 2020, 182, pp. 116041
Issue Date:: 2020-09

Closed Access

	Filename	Description	Size
	1-s2.0-S0043135420305789-main.pdf	Published version	2.73 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Wei, W
dc.contributor.author	Hao, Q https://orcid.org/0000-0001-9981-7499
dc.contributor.author	Chen, Z https://orcid.org/0000-0003-0627-0856
dc.contributor.author	Bao, T
dc.contributor.author	Ni, B-J
dc.date.accessioned	2021-03-28T07:43:04Z
dc.date.available	2020-06-10
dc.date.available	2021-03-28T07:43:04Z
dc.date.issued	2020-09
dc.identifier.citation	Water research, 2020, 182, pp. 116041
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/147568
dc.description.abstract	Wastewater has been identified as an important carrier for nanoplastics, which could elicit unintended impacts on critical microbial processes. However, the long-term impacts of nanoplastics on anaerobic granular sludge (AGS) for methane recovery from wastewater and the mechanisms involved remains unclear. In this study, we investigated the long term exposure-response relationship between polystyrene nanoplastics (Nano-PS) and AGS. In continuous test over 120 days with 86 days' Nano-PS exposure, feeding wastewater with 10 μg/L of Nano-PS had no significant impacts on the AGS performance. In comparison, higher levels (i.e., 20 and 50 μg/L) of Nano-PS decreased methane production and chemical oxygen demand (COD) removal by 19.0-28.6% and 19.3-30.0%, respectively, along with volatile fatty acids (VFA) accumulation. More extracellular polymeric substance (EPS) was induced by 10 μg/L of Nano-PS as a response to protect microbes, but higher levels (i.e., 20 and 50 μg/L) of Nano-PS decreased EPS generation, causing a decline in granule size and cell viability. Fluorescence tagging found that a large number of Nano-PS agglomerated/accumulated on the outer layer of AGS and even transferred into deeper layers of AGS over exposure time, producing toxic effects to adherent microorganisms, e.g., Longilinea sp., Paludibacter sp. and Methanosaeta sp.. The oxidative stress induced by Nano-PS was revealed to be a key factor for reshaping the AGS, reflected by the increased reactive oxygen species (ROS) generation and lactate dehydrogenase (LDH) release. The sodium dodecyl sulfate (SDS) leached from Nano-PS was also demonstrated to restrain the activities of antioxidant enzymes, thereby further lessening resistance to oxidative stress induced by Nano-PS. This work improves our ability to predict the risks associated with this ubiquitous contaminant in the environment.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation	http://purl.org/au-research/grants/arc/FT160100195
dc.relation.ispartof	Water research
dc.relation.isbasedon	10.1016/j.watres.2020.116041
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	Methane
dc.subject.mesh	Polystyrenes
dc.subject.mesh	Bioreactors
dc.subject.mesh	Sewage
dc.subject.mesh	Waste Disposal, Fluid
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Waste Water
dc.subject.mesh	Extracellular Polymeric Substance Matrix
dc.subject.mesh	Microplastics
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Bioreactors
dc.subject.mesh	Extracellular Polymeric Substance Matrix
dc.subject.mesh	Methane
dc.subject.mesh	Microplastics
dc.subject.mesh	Polystyrenes
dc.subject.mesh	Sewage
dc.subject.mesh	Waste Disposal, Fluid
dc.subject.mesh	Waste Water
dc.title	Polystyrene nanoplastics reshape the anaerobic granular sludge for recovering methane from wastewater.
dc.type	Journal Article
utslib.citation.volume	182
utslib.location.activity	England
utslib.for	090409 Wastewater Treatment Processes
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access	*
dc.date.updated	2021-03-28T07:42:59Z
pubs.publication-status	Published
pubs.volume	182

Abstract:

Wastewater has been identified as an important carrier for nanoplastics, which could elicit unintended impacts on critical microbial processes. However, the long-term impacts of nanoplastics on anaerobic granular sludge (AGS) for methane recovery from wastewater and the mechanisms involved remains unclear. In this study, we investigated the long term exposure-response relationship between polystyrene nanoplastics (Nano-PS) and AGS. In continuous test over 120 days with 86 days' Nano-PS exposure, feeding wastewater with 10 μg/L of Nano-PS had no significant impacts on the AGS performance. In comparison, higher levels (i.e., 20 and 50 μg/L) of Nano-PS decreased methane production and chemical oxygen demand (COD) removal by 19.0-28.6% and 19.3-30.0%, respectively, along with volatile fatty acids (VFA) accumulation. More extracellular polymeric substance (EPS) was induced by 10 μg/L of Nano-PS as a response to protect microbes, but higher levels (i.e., 20 and 50 μg/L) of Nano-PS decreased EPS generation, causing a decline in granule size and cell viability. Fluorescence tagging found that a large number of Nano-PS agglomerated/accumulated on the outer layer of AGS and even transferred into deeper layers of AGS over exposure time, producing toxic effects to adherent microorganisms, e.g., Longilinea sp., Paludibacter sp. and Methanosaeta sp.. The oxidative stress induced by Nano-PS was revealed to be a key factor for reshaping the AGS, reflected by the increased reactive oxygen species (ROS) generation and lactate dehydrogenase (LDH) release. The sodium dodecyl sulfate (SDS) leached from Nano-PS was also demonstrated to restrain the activities of antioxidant enzymes, thereby further lessening resistance to oxidative stress induced by Nano-PS. This work improves our ability to predict the risks associated with this ubiquitous contaminant in the environment.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/147568