Investigating MIF in Mouse Models of Severe Corticosteroid-Resistant Neutrophilic Asthma.

Allam, VSRR; Sukkar, MB

Investigating MIF in Mouse Models of Severe Corticosteroid-Resistant Neutrophilic Asthma.

Allam, VSRR Sukkar, MB

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Publisher:: Springer US
Publication Type:: Journal Article
Citation:: Methods in molecular biology (Clifton, N.J.), 2020, 2080, pp. 203-212
Issue Date:: 2020-01

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Field	Value	Language
dc.contributor.author	Allam, VSRR
dc.contributor.author	Sukkar, MB
dc.date.accessioned	2021-04-09T04:57:59Z
dc.date.available	2021-04-09T04:57:59Z
dc.date.issued	2020-01
dc.identifier.citation	Methods in molecular biology (Clifton, N.J.), 2020, 2080, pp. 203-212
dc.identifier.isbn	9781493999354
dc.identifier.issn	1064-3745
dc.identifier.issn	1940-6029
dc.identifier.uri	http://hdl.handle.net/10453/147951
dc.description.abstract	Experimental mouse models of asthma are widely used to investigate the underlying mechanisms of this complex and heterogeneous disease. Using mouse models of ovalbumin-induced asthma, previous investigators have established a crucial role for MIF in the development of type 2-mediated eosinophilic asthma. Surprisingly, however, the role of MIF in other phenotypes of asthma has received little attention. MIF is an important mediator of neutrophilic inflammation, and also acts to antagonize the actions of corticosteroids. Thus, MIF may play a role in the development of severe forms of asthma in which airway neutrophilia and corticosteroid insensitivity are major features. In this chapter, we provide an experimental protocol that may be used to investigate the role of MIF in a mouse model of severe corticosteroid-resistant neutrophilic asthma.
dc.format	Print
dc.language	eng
dc.publisher	Springer US
dc.relation.ispartof	Methods in molecular biology (Clifton, N.J.)
dc.relation.isbasedon	10.1007/978-1-4939-9936-1_18
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.mesh	Neutrophils
dc.subject.mesh	Animals
dc.subject.mesh	Mice
dc.subject.mesh	Asthma
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Isoxazoles
dc.subject.mesh	Dexamethasone
dc.subject.mesh	Adrenal Cortex Hormones
dc.subject.mesh	Intramolecular Oxidoreductases
dc.subject.mesh	Antigens, Dermatophagoides
dc.subject.mesh	Macrophage Migration-Inhibitory Factors
dc.subject.mesh	Severity of Illness Index
dc.subject.mesh	Drug Resistance
dc.subject.mesh	Adrenal Cortex Hormones
dc.subject.mesh	Animals
dc.subject.mesh	Antigens, Dermatophagoides
dc.subject.mesh	Asthma
dc.subject.mesh	Dexamethasone
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Drug Resistance
dc.subject.mesh	Intramolecular Oxidoreductases
dc.subject.mesh	Isoxazoles
dc.subject.mesh	Macrophage Migration-Inhibitory Factors
dc.subject.mesh	Mice
dc.subject.mesh	Neutrophils
dc.subject.mesh	Severity of Illness Index
dc.title	Investigating MIF in Mouse Models of Severe Corticosteroid-Resistant Neutrophilic Asthma.
dc.type	Journal Article
utslib.citation.volume	2080
utslib.location.activity	United States
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
utslib.copyright.status	recently_added	*
dc.date.updated	2021-04-09T04:57:58Z
pubs.publication-status	Published
pubs.volume	2080

Abstract:

Experimental mouse models of asthma are widely used to investigate the underlying mechanisms of this complex and heterogeneous disease. Using mouse models of ovalbumin-induced asthma, previous investigators have established a crucial role for MIF in the development of type 2-mediated eosinophilic asthma. Surprisingly, however, the role of MIF in other phenotypes of asthma has received little attention. MIF is an important mediator of neutrophilic inflammation, and also acts to antagonize the actions of corticosteroids. Thus, MIF may play a role in the development of severe forms of asthma in which airway neutrophilia and corticosteroid insensitivity are major features. In this chapter, we provide an experimental protocol that may be used to investigate the role of MIF in a mouse model of severe corticosteroid-resistant neutrophilic asthma.

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http://hdl.handle.net/10453/147951