Enhancement in brain uptake of vitamin D<sub>3</sub> nanoemulsion for treatment of cerebral ischemia: formulation, gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models.

Kumar, M; Nishad, DK; Kumar, A; Bhatnagar, A; Karwasra, R; Khanna, K; S, K; Sharma, D; Dua, K; Mudaliyar, V; Sharma, N

Enhancement in brain uptake of vitamin D<sub>3</sub> nanoemulsion for treatment of cerebral ischemia: formulation, gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models.

Kumar, M Nishad, DK Kumar, A Bhatnagar, A Karwasra, R Khanna, K S, K Sharma, D Dua, K

Mudaliyar, V Sharma, N

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Publisher:: TAYLOR & FRANCIS LTD
Publication Type:: Journal Article
Citation:: Journal of microencapsulation, 2020, 37, (7), pp. 492-501
Issue Date:: 2020-11

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Field	Value	Language
dc.contributor.author	Kumar, M
dc.contributor.author	Nishad, DK
dc.contributor.author	Kumar, A
dc.contributor.author	Bhatnagar, A
dc.contributor.author	Karwasra, R
dc.contributor.author	Khanna, K
dc.contributor.author	S, K
dc.contributor.author	Sharma, D
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Mudaliyar, V
dc.contributor.author	Sharma, N
dc.date.accessioned	2021-04-13T06:27:26Z
dc.date.available	2021-04-13T06:27:26Z
dc.date.issued	2020-11
dc.identifier.citation	Journal of microencapsulation, 2020, 37, (7), pp. 492-501
dc.identifier.issn	0265-2048
dc.identifier.issn	1464-5246
dc.identifier.uri	http://hdl.handle.net/10453/148061
dc.description.abstract	<h4>Aim</h4>For the treatment of cerebral ischaemia, vitamin-D<sub>3</sub> loaded nanoemulsions were developed.<h4>Method</h4>Tween 20 and polyethylene glycol were chosen as surfactant/co-surfactant, while oleic acid as the oil phase. The formulation was characterised for various <i>in-vitro</i> parameters. Targeting efficiency was investigated through radiometry, gamma scintigraphy and efficacy was studied in transient middle cerebral artery occlusion (MCAo) rat model.<h4>Result</h4>Vitamin D<sub>3</sub>-nanoemulsion showed a mean size range of 49.29 ± 10.28 nm with polydispersity index 0.17 ± 0.04 and zeta potential 13.77 mV. The formulation was found stable during thermodynamic stability study and permeated within 180 min through sheep nasal mucosa (permeation coefficient 7.873 ± 0.884 cm/h). Gamma scintigraphy and radiometry assay confirmed better percentage deposition (2.53 ± 0.17%) of <sup>99m</sup>Tc-vitamin D<sub>3</sub>-nanoemulsion through nasal route compared to IV administered <sup>99m</sup>Tc-vitamin D<sub>3</sub> solution (0.79 ± 0.03%). Magnetic Resonance Imaging (MRI) of the ischaemic model confirmed better efficacy of vitamin D<sub>3</sub>-nanoemulsion.<h4>Conclusion</h4>This work demonstrated better permeation, deposition, and efficacy of vitaminD<sub>3</sub>-nanoemulsion through the intranasal route.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	TAYLOR & FRANCIS LTD
dc.relation.ispartof	Journal of microencapsulation
dc.relation.isbasedon	10.1080/02652048.2020.1801870
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Pharmacology & Pharmacy
dc.title	Enhancement in brain uptake of vitamin D<sub>3</sub> nanoemulsion for treatment of cerebral ischemia: formulation, gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models.
dc.type	Journal Article
utslib.citation.volume	37
utslib.location.activity	England
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2021-04-13T06:27:25Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	37
utslib.citation.issue	7

Abstract:

Aim

For the treatment of cerebral ischaemia, vitamin-D₃ loaded nanoemulsions were developed.

Method

Tween 20 and polyethylene glycol were chosen as surfactant/co-surfactant, while oleic acid as the oil phase. The formulation was characterised for various in-vitro parameters. Targeting efficiency was investigated through radiometry, gamma scintigraphy and efficacy was studied in transient middle cerebral artery occlusion (MCAo) rat model.

Result

Vitamin D₃-nanoemulsion showed a mean size range of 49.29 ± 10.28 nm with polydispersity index 0.17 ± 0.04 and zeta potential 13.77 mV. The formulation was found stable during thermodynamic stability study and permeated within 180 min through sheep nasal mucosa (permeation coefficient 7.873 ± 0.884 cm/h). Gamma scintigraphy and radiometry assay confirmed better percentage deposition (2.53 ± 0.17%) of ^99mTc-vitamin D₃-nanoemulsion through nasal route compared to IV administered ^99mTc-vitamin D₃ solution (0.79 ± 0.03%). Magnetic Resonance Imaging (MRI) of the ischaemic model confirmed better efficacy of vitamin D₃-nanoemulsion.

Conclusion

This work demonstrated better permeation, deposition, and efficacy of vitaminD₃-nanoemulsion through the intranasal route.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/148061