Down-regulation of miR-17 family expression in response to retinoic acid induced neuronal differentiation

Beveridge, NJ; Tooney, PA; Carroll, AP; Tran, N; Cairns, MJ

Down-regulation of miR-17 family expression in response to retinoic acid induced neuronal differentiation

Beveridge, NJ Tooney, PA Carroll, AP Tran, N

Cairns, MJ

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Publication Type:: Journal Article
Citation:: Cellular Signalling, 2009, 21 (12), pp. 1837 - 1845
Issue Date:: 2009-12-01

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Field	Value	Language
dc.contributor.author	Beveridge, NJ	en_US
dc.contributor.author	Tooney, PA	en_US
dc.contributor.author	Carroll, AP	en_US
dc.contributor.author	Tran, N https://orcid.org/0000-0001-7747-2530	en_US
dc.contributor.author	Cairns, MJ	en_US
dc.date.available	2009-07-30	en_US
dc.date.issued	2009-12-01	en_US
dc.identifier.citation	Cellular Signalling, 2009, 21 (12), pp. 1837 - 1845	en_US
dc.identifier.issn	0898-6568	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14979
dc.description.abstract	Whole-genome microRNA and gene expression analyses were used to monitor changes during retinoic acid induced differentiation of neuroblasts in vitro. Interestingly, the entire miR-17 family was over-represented among the down-regulated miRNA. The implications of these changes are considerable, as target gene prediction suggests that the miR-17 family is involved in the regulation of the mitogen-activated protein kinase (MAPK) signaling pathway, synaptic plasticity and other markers of neuronal differentiation. Significantly, many of the target responses predicted by changes in miRNA expression were supported by the observed changes in gene expression. As expected, markers of neuronal differentiation such as anti-apoptotic protein B-cell lymphoma 2 (BCL2), myocyte enhancer factor-2D (MEF2D) and zipper protein kinase (MAP3K12; aka ZPK/MUK/DLK) were each up-regulated in response to differentiation. The expression of these genes was also reduced in response to miR-17 and miR-20a transfection, and more specifically they were also shown to contain functional miRNA recognition elements for members of the miR-17 family by reporter gene assay. This suggests that the miR-17 family have an integral role in fine-tuning the pathways involved in the regulation of neuronal differentiation. © 2009 Elsevier Inc. All rights reserved.	en_US
dc.relation.ispartof	Cellular Signalling	en_US
dc.relation.isbasedon	10.1016/j.cellsig.2009.07.019	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Neuroblastoma	en_US
dc.subject.mesh	Tretinoin	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Down-Regulation	en_US
dc.subject.mesh	Gene Expression Regulation, Neoplastic	en_US
dc.subject.mesh	Neurogenesis	en_US
dc.title	Down-regulation of miR-17 family expression in response to retinoic acid induced neuronal differentiation	en_US
dc.type	Journal Article
utslib.citation.volume	12	en_US
utslib.citation.volume	21	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1116 Medical Physiology	en_US
dc.location.activity	England
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	12	en_US
pubs.publication-status	Published	en_US
pubs.volume	21	en_US

Abstract:

Whole-genome microRNA and gene expression analyses were used to monitor changes during retinoic acid induced differentiation of neuroblasts in vitro. Interestingly, the entire miR-17 family was over-represented among the down-regulated miRNA. The implications of these changes are considerable, as target gene prediction suggests that the miR-17 family is involved in the regulation of the mitogen-activated protein kinase (MAPK) signaling pathway, synaptic plasticity and other markers of neuronal differentiation. Significantly, many of the target responses predicted by changes in miRNA expression were supported by the observed changes in gene expression. As expected, markers of neuronal differentiation such as anti-apoptotic protein B-cell lymphoma 2 (BCL2), myocyte enhancer factor-2D (MEF2D) and zipper protein kinase (MAP3K12; aka ZPK/MUK/DLK) were each up-regulated in response to differentiation. The expression of these genes was also reduced in response to miR-17 and miR-20a transfection, and more specifically they were also shown to contain functional miRNA recognition elements for members of the miR-17 family by reporter gene assay. This suggests that the miR-17 family have an integral role in fine-tuning the pathways involved in the regulation of neuronal differentiation. © 2009 Elsevier Inc. All rights reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/14979