Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways

Schwarz, DS; Hutvágner, G; Haley, B; Zamore, PD

Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways

Schwarz, DS Hutvágner, G

Haley, B Zamore, PD

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Publication Type:: Journal Article
Citation:: Molecular Cell, 2002, 10 (3), pp. 537 - 548
Issue Date:: 2002-09-01

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Field	Value	Language
dc.contributor.author	Schwarz, DS	en_US
dc.contributor.author	Hutvágner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.contributor.author	Haley, B	en_US
dc.contributor.author	Zamore, PD	en_US
dc.date.issued	2002-09-01	en_US
dc.identifier.citation	Molecular Cell, 2002, 10 (3), pp. 537 - 548	en_US
dc.identifier.issn	1097-2765	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14988
dc.description.abstract	In Drosophila, two features of small interfering RNA (siRNA) structure - 5′ phosphates and 3′ hydroxyls - are reported to be essential for RNA interference (RNAi). Here, we show that as in Drosophila, a 5′ phosphate is required for siRNA function in human HeLa cells. In contrast, we find no evidence in flies or humans for a role in RNAi for the siRNA 3′ hydroxyl group. Our in vitro data suggest that in both flies and mammals, each siRNA guides endonucleolytic cleavage of the target RNA at a single site. We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms.	en_US
dc.relation.ispartof	Molecular Cell	en_US
dc.relation.isbasedon	10.1016/S1097-2765(02)00651-2	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Hela Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Drosophila melanogaster	en_US
dc.subject.mesh	Phosphates	en_US
dc.subject.mesh	Endoribonucleases	en_US
dc.subject.mesh	Ribonuclease III	en_US
dc.subject.mesh	RNA, Small Interfering	en_US
dc.subject.mesh	RNA, Double-Stranded	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	RNA Interference	en_US
dc.subject.mesh	Genes, Insect	en_US
dc.subject.mesh	HeLa Cells	en_US
dc.title	Evidence that siRNAs function as guides, not primers, in the Drosophila and human RNAi pathways	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	10	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000178330900012	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

In Drosophila, two features of small interfering RNA (siRNA) structure - 5′ phosphates and 3′ hydroxyls - are reported to be essential for RNA interference (RNAi). Here, we show that as in Drosophila, a 5′ phosphate is required for siRNA function in human HeLa cells. In contrast, we find no evidence in flies or humans for a role in RNAi for the siRNA 3′ hydroxyl group. Our in vitro data suggest that in both flies and mammals, each siRNA guides endonucleolytic cleavage of the target RNA at a single site. We conclude that the underlying mechanism of RNAi is conserved between flies and mammals and that RNA-dependent RNA polymerases are not required for RNAi in these organisms.

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http://hdl.handle.net/10453/14988