A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA
- Publication Type:
- Journal Article
- Citation:
- Science, 2001, 293 (5531), pp. 834 - 838
- Issue Date:
- 2001-08-03
Closed Access
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2010003466OK.pdf | 71.57 kB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author |
Hutvágner, G https://orcid.org/0000-0002-7231-9446 |
en_US |
dc.contributor.author | McLachlan, J | en_US |
dc.contributor.author | Pasquinelli, AE | en_US |
dc.contributor.author | Bálint, E | en_US |
dc.contributor.author | Tuschl, T | en_US |
dc.contributor.author | Zamore, PD | en_US |
dc.date.issued | 2001-08-03 | en_US |
dc.identifier.citation | Science, 2001, 293 (5531), pp. 834 - 838 | en_US |
dc.identifier.issn | 0036-8075 | en_US |
dc.identifier.uri | http://hdl.handle.net/10453/14989 | |
dc.description.abstract | The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression. | en_US |
dc.relation.ispartof | Science | en_US |
dc.relation.isbasedon | 10.1126/science.1062961 | en_US |
dc.subject.classification | General Science & Technology | en_US |
dc.subject.mesh | Hela Cells | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Drosophila melanogaster | en_US |
dc.subject.mesh | Endoribonucleases | en_US |
dc.subject.mesh | Ribonuclease III | en_US |
dc.subject.mesh | RNA Precursors | en_US |
dc.subject.mesh | RNA, Double-Stranded | en_US |
dc.subject.mesh | RNA, Helminth | en_US |
dc.subject.mesh | RNA, Messenger | en_US |
dc.subject.mesh | Blotting, Northern | en_US |
dc.subject.mesh | Transfection | en_US |
dc.subject.mesh | Transcription, Genetic | en_US |
dc.subject.mesh | Gene Expression Regulation, Developmental | en_US |
dc.subject.mesh | RNA Processing, Post-Transcriptional | en_US |
dc.subject.mesh | Nucleic Acid Conformation | en_US |
dc.subject.mesh | Protein Structure, Tertiary | en_US |
dc.subject.mesh | HeLa Cells | en_US |
dc.title | A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA | en_US |
dc.type | Journal Article | |
utslib.citation.volume | 5531 | en_US |
utslib.citation.volume | 293 | en_US |
utslib.for | 060107 Enzymes | en_US |
dc.location.activity | ISI:000170241000037 | en_US |
pubs.embargo.period | Not known | en_US |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Strength - CHT - Health Technologies | |
utslib.copyright.status | closed_access | |
pubs.issue | 5531 | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 293 | en_US |
Abstract:
The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.
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