A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA

Publication Type:
Journal Article
Citation:
Science, 2001, 293 (5531), pp. 834 - 838
Issue Date:
2001-08-03
Metrics:
Full metadata record
Files in This Item:
Filename Description Size
Thumbnail2010003466OK.pdf71.57 kB
Adobe PDF
The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.
Please use this identifier to cite or link to this item: