A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA

Hutvágner, G; McLachlan, J; Pasquinelli, AE; Bálint, E; Tuschl, T; Zamore, PD

A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA

Hutvágner, G

McLachlan, J Pasquinelli, AE Bálint, E Tuschl, T Zamore, PD

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Publication Type:: Journal Article
Citation:: Science, 2001, 293 (5531), pp. 834 - 838
Issue Date:: 2001-08-03

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Field	Value	Language
dc.contributor.author	Hutvágner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.contributor.author	McLachlan, J	en_US
dc.contributor.author	Pasquinelli, AE	en_US
dc.contributor.author	Bálint, E	en_US
dc.contributor.author	Tuschl, T	en_US
dc.contributor.author	Zamore, PD	en_US
dc.date.issued	2001-08-03	en_US
dc.identifier.citation	Science, 2001, 293 (5531), pp. 834 - 838	en_US
dc.identifier.issn	0036-8075	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14989
dc.description.abstract	The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.	en_US
dc.relation.ispartof	Science	en_US
dc.relation.isbasedon	10.1126/science.1062961	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Hela Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Drosophila melanogaster	en_US
dc.subject.mesh	Endoribonucleases	en_US
dc.subject.mesh	Ribonuclease III	en_US
dc.subject.mesh	RNA Precursors	en_US
dc.subject.mesh	RNA, Double-Stranded	en_US
dc.subject.mesh	RNA, Helminth	en_US
dc.subject.mesh	RNA, Messenger	en_US
dc.subject.mesh	Blotting, Northern	en_US
dc.subject.mesh	Transfection	en_US
dc.subject.mesh	Transcription, Genetic	en_US
dc.subject.mesh	Gene Expression Regulation, Developmental	en_US
dc.subject.mesh	RNA Processing, Post-Transcriptional	en_US
dc.subject.mesh	Nucleic Acid Conformation	en_US
dc.subject.mesh	Protein Structure, Tertiary	en_US
dc.subject.mesh	HeLa Cells	en_US
dc.title	A cellular function for the RNA-interference enzyme dicer in the maturation of the let-7 small temporal RNA	en_US
dc.type	Journal Article
utslib.citation.volume	5531	en_US
utslib.citation.volume	293	en_US
utslib.for	060107 Enzymes	en_US
dc.location.activity	ISI:000170241000037	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	5531	en_US
pubs.publication-status	Published	en_US
pubs.volume	293	en_US

Abstract:

The 21-nucleotide small temporal RNA (stRNA) let-7 regulates developmental timing in Caenorhabditis elegans and probably in other bilateral animals. We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. Thus, the RNA interference and stRNA pathways intersect. Both pathways require the RNA-processing enzyme Dicer to produce the active small-RNA component that represses gene expression.

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http://hdl.handle.net/10453/14989