Comparative in vitro evaluation of glimepiride containing nanosuspension drug delivery system developed by different techniques

Bose, S; Sharma, P; Mishra, V; Patial, S; Saraogi, GK; Tambuwala, MM; Dua, K

Comparative in vitro evaluation of glimepiride containing nanosuspension drug delivery system developed by different techniques

Bose, S Sharma, P Mishra, V Patial, S Saraogi, GK Tambuwala, MM Dua, K

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Journal of Molecular Structure, 2021, 1231
Issue Date:: 2021-05-05

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Field	Value	Language
dc.contributor.author	Bose, S
dc.contributor.author	Sharma, P
dc.contributor.author	Mishra, V
dc.contributor.author	Patial, S
dc.contributor.author	Saraogi, GK
dc.contributor.author	Tambuwala, MM
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.date.accessioned	2021-09-14T03:43:52Z
dc.date.available	2021-09-14T03:43:52Z
dc.date.issued	2021-05-05
dc.identifier.citation	Journal of Molecular Structure, 2021, 1231
dc.identifier.issn	0022-2860
dc.identifier.issn	1872-8014
dc.identifier.uri	http://hdl.handle.net/10453/150530
dc.description.abstract	The present study was aimed to develop the Glimepiride (GLM) loaded nanosuspension by different methods for increasing the solubility of GLM. Twelve formulations were prepared by combination method (FG), which included antisolvent precipitation method followed by sonication (Method 1) selecting drug and polymer in ratio of 1:10, 1:20 and 1:30. Further 6 formulations were prepared by nanoprecipitation method (Fg) (Method 2) selecting drug and polymer in ratio of 1:10, 1:20 by using different polymers like polyvinyl pyrolidone (PVP K30), and poly(ethylene glycol) (PEG) such as PEG 6000 and PEG 400. The GLM nanosuspensions prepared by different techniques were evaluated by optical microscopy, percent entrapment efficiency (%EE), particle size analysis, zeta potential, transmission electron microscopy (TEM) and in vitro dissolution. FG1 formulation was found to be better formulation showing 82.04% EE, 129–180 nm particle size range, 30.16 mV zeta potential, 0.253 polydispersity index (PDI) and 86.76% drug release as compared to Fgii formulation having %EE, average particle size, zeta potential, PDI value and drug release as 80.03%, 72–383 nm, -22.19 mV, 0.358 and 74.77%, respectively. On the basis of results obtained from different studies, it can be concluded that GLM nanosuspension prepared by combination technique (FG) shows good solubility and dissolution than those prepared by nanoprecipitation technique (Fg), hence the combination technique is found to be a preferred technique to prepare GLM nanosuspension over nanoprecipitation technique.
dc.language	en
dc.publisher	Elsevier BV
dc.relation.ispartof	Journal of Molecular Structure
dc.relation.isbasedon	10.1016/j.molstruc.2021.129927
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0306 Physical Chemistry (incl. Structural), 0307 Theoretical and Computational Chemistry
dc.subject.classification	Inorganic & Nuclear Chemistry
dc.title	Comparative in vitro evaluation of glimepiride containing nanosuspension drug delivery system developed by different techniques
dc.type	Journal Article
utslib.citation.volume	1231
utslib.for	0306 Physical Chemistry (incl. Structural)
utslib.for	0307 Theoretical and Computational Chemistry
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2021-09-14T03:43:51Z
pubs.publication-status	Published
pubs.volume	1231

Abstract:

The present study was aimed to develop the Glimepiride (GLM) loaded nanosuspension by different methods for increasing the solubility of GLM. Twelve formulations were prepared by combination method (FG), which included antisolvent precipitation method followed by sonication (Method 1) selecting drug and polymer in ratio of 1:10, 1:20 and 1:30. Further 6 formulations were prepared by nanoprecipitation method (Fg) (Method 2) selecting drug and polymer in ratio of 1:10, 1:20 by using different polymers like polyvinyl pyrolidone (PVP K30), and poly(ethylene glycol) (PEG) such as PEG 6000 and PEG 400. The GLM nanosuspensions prepared by different techniques were evaluated by optical microscopy, percent entrapment efficiency (%EE), particle size analysis, zeta potential, transmission electron microscopy (TEM) and in vitro dissolution. FG1 formulation was found to be better formulation showing 82.04% EE, 129–180 nm particle size range, 30.16 mV zeta potential, 0.253 polydispersity index (PDI) and 86.76% drug release as compared to Fgii formulation having %EE, average particle size, zeta potential, PDI value and drug release as 80.03%, 72–383 nm, -22.19 mV, 0.358 and 74.77%, respectively. On the basis of results obtained from different studies, it can be concluded that GLM nanosuspension prepared by combination technique (FG) shows good solubility and dissolution than those prepared by nanoprecipitation technique (Fg), hence the combination technique is found to be a preferred technique to prepare GLM nanosuspension over nanoprecipitation technique.

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