Biocatalytic micromixer coated with enzyme-MOF thin film for CO<inf>2</inf> conversion to formic acid

Chai, M; Razavi Bazaz, S; Daiyan, R; Razmjou, A; Ebrahimi Warkiani, M; Amal, R; Chen, V

Biocatalytic micromixer coated with enzyme-MOF thin film for CO<inf>2</inf> conversion to formic acid

Chai, M Razavi Bazaz, S Daiyan, R Razmjou, A Ebrahimi Warkiani, M

Amal, R Chen, V

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Chemical Engineering Journal, 2021, 426, pp. 130856-130856
Issue Date:: 2021-12-15

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Field	Value	Language
dc.contributor.author	Chai, M
dc.contributor.author	Razavi Bazaz, S
dc.contributor.author	Daiyan, R
dc.contributor.author	Razmjou, A
dc.contributor.author	Ebrahimi Warkiani, M https://orcid.org/0000-0002-4184-1944
dc.contributor.author	Amal, R
dc.contributor.author	Chen, V
dc.date.accessioned	2021-10-20T04:14:10Z
dc.date.available	2021-10-20T04:14:10Z
dc.date.issued	2021-12-15
dc.identifier.citation	Chemical Engineering Journal, 2021, 426, pp. 130856-130856
dc.identifier.issn	1385-8947
dc.identifier.uri	http://hdl.handle.net/10453/151158
dc.description.abstract	In this study, a novel micromixer with a 3D helical, threaded channel was fabricated via 3D printing. The micromixer can enhance the mass transfer of reactants and product in an enzymatic cascade reaction converting CO2 to formic acid. Two enzymes, including carbonic anhydrase (CA) and formate dehydrogenase (FDH), were biomineralised in a zeolitic imidazolate framework-8 composite thin film on the micromixer channel that has been modified with polydopamine/polyethyleneimine. The biocatalytic performance of the micromixer was evaluated by testing at various liquid flow rates, and an optimum liquid flow rate at 1 mL/min (Rel = 8, Del = 3) was observed as the two-phase flow pattern in the micromixer channel transitioned from slug flow to bubbly flow. A comparison of the micromixer performance with and without threaded channels revealed ~ 170% enhancement in formic acid yield, indicating improved mixing with the presence of threads. In addition, the formic acid production rate for the micromixer with threaded channel was three folds higher than a conventional bubble column, demonstrating the superior performance of the proposed micromixer. The ease of assembling multiple micromixer units in series also enabled the immobilisation of different enzymes in separate units to carry out sequential reactions in a modular system. As a proof of concept, the solution product collected from long term biocatalysis was also tested in a direct formic acid fuel cell, which showed a promising prospect of integrating these two systems for a closed-loop energy generation system.
dc.language	en
dc.publisher	Elsevier BV
dc.relation.ispartof	Chemical Engineering Journal
dc.relation.isbasedon	10.1016/j.cej.2021.130856
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0904 Chemical Engineering, 0905 Civil Engineering, 0907 Environmental Engineering
dc.subject.classification	Chemical Engineering
dc.title	Biocatalytic micromixer coated with enzyme-MOF thin film for CO<inf>2</inf> conversion to formic acid
dc.type	Journal Article
utslib.citation.volume	426
utslib.for	0904 Chemical Engineering
utslib.for	0905 Civil Engineering
utslib.for	0907 Environmental Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	closed_access	*
dc.date.updated	2021-10-20T04:14:08Z
pubs.publication-status	Accepted
pubs.volume	426

Abstract:

In this study, a novel micromixer with a 3D helical, threaded channel was fabricated via 3D printing. The micromixer can enhance the mass transfer of reactants and product in an enzymatic cascade reaction converting CO2 to formic acid. Two enzymes, including carbonic anhydrase (CA) and formate dehydrogenase (FDH), were biomineralised in a zeolitic imidazolate framework-8 composite thin film on the micromixer channel that has been modified with polydopamine/polyethyleneimine. The biocatalytic performance of the micromixer was evaluated by testing at various liquid flow rates, and an optimum liquid flow rate at 1 mL/min (Rel = 8, Del = 3) was observed as the two-phase flow pattern in the micromixer channel transitioned from slug flow to bubbly flow. A comparison of the micromixer performance with and without threaded channels revealed ~ 170% enhancement in formic acid yield, indicating improved mixing with the presence of threads. In addition, the formic acid production rate for the micromixer with threaded channel was three folds higher than a conventional bubble column, demonstrating the superior performance of the proposed micromixer. The ease of assembling multiple micromixer units in series also enabled the immobilisation of different enzymes in separate units to carry out sequential reactions in a modular system. As a proof of concept, the solution product collected from long term biocatalysis was also tested in a direct formic acid fuel cell, which showed a promising prospect of integrating these two systems for a closed-loop energy generation system.

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http://hdl.handle.net/10453/151158