Mechanobiology of Dental Pulp Stem Cells at the Interface of Aqueous-Based Fabricated ZIF8 Thin Film

Ejeian, F; Haghani, E; Nasr-Esfahani, MH; Asadnia, M; Razmjou, A; Chen, V

Mechanobiology of Dental Pulp Stem Cells at the Interface of Aqueous-Based Fabricated ZIF8 Thin Film

Ejeian, F Haghani, E Nasr-Esfahani, MH Asadnia, M Razmjou, A Chen, V

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Publisher:: American Chemical Society (ACS)
Publication Type:: Journal Article
Citation:: ACS Applied Bio Materials, 2021, 4, (6), pp. 4885-4895
Issue Date:: 2021-06-21

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Field	Value	Language
dc.contributor.author	Ejeian, F
dc.contributor.author	Haghani, E
dc.contributor.author	Nasr-Esfahani, MH
dc.contributor.author	Asadnia, M
dc.contributor.author	Razmjou, A
dc.contributor.author	Chen, V
dc.date.accessioned	2021-10-20T05:23:41Z
dc.date.available	2021-10-20T05:23:41Z
dc.date.issued	2021-06-21
dc.identifier.citation	ACS Applied Bio Materials, 2021, 4, (6), pp. 4885-4895
dc.identifier.issn	2576-6422
dc.identifier.issn	2576-6422
dc.identifier.uri	http://hdl.handle.net/10453/151171
dc.description.abstract	The limited knowledge on how biological systems sense and respond to the mechanical properties of metal-organic framework (MOF) thin films is a critical restriction factor for their extensive usage. To bridge this gap, we performed an in vitro study for defining and linking surface characteristics at the interface of the zeolitic imidazolate framework-8 (ZIF8) thin layer to stem cell behavior. First, the physio-mechanical properties of the ZIF8 layer grown on polydopamine (PDA) and tannic acid (TA) layers have been studied. The response of dental pulp stem cells (DPSCs) to different surface states was examined. The results showed that the uniform crystalline microstructure of the ZIF8 on PDA and TA effectively led to the 61- and 388-fold increased surface roughness, 3- and 2.5-fold moderated elastic modulus, almost 3-fold elevated surface free energy, and highly charged surfaces (ζ = -60 mV for TA/ZIF8), respectively. Beyond the inherent bioactivity of the ZIF8 layer, these substrate cues promoted advanced cell adhesion (∼two times) and high proliferation rate. Furthermore, we found a substantial increment in the differentiation efficiency of DPSCs on the ZIF8 layer, in a way that the expression of functional adipocyte (PPARG) and osteoblast (SPP1) markers was, respectively, elevated around 30 »000- and 10 »000-fold on the TA/ZIF8-coated silicon wafer (SW). Our findings support the impact of fabrication strategy on the biointerface properties of the ZIF8 layer and bring SW/TA/ZIF8 as a robust platform for managing stem cells for biomedical applications.
dc.language	en
dc.publisher	American Chemical Society (ACS)
dc.relation.ispartof	ACS Applied Bio Materials
dc.relation.isbasedon	10.1021/acsabm.1c00189
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Mechanobiology of Dental Pulp Stem Cells at the Interface of Aqueous-Based Fabricated ZIF8 Thin Film
dc.type	Journal Article
utslib.citation.volume	4
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
utslib.copyright.status	closed_access	*
dc.date.updated	2021-10-20T05:23:39Z
pubs.issue	6
pubs.publication-status	Published
pubs.volume	4
utslib.citation.issue	6

Abstract:

The limited knowledge on how biological systems sense and respond to the mechanical properties of metal-organic framework (MOF) thin films is a critical restriction factor for their extensive usage. To bridge this gap, we performed an in vitro study for defining and linking surface characteristics at the interface of the zeolitic imidazolate framework-8 (ZIF8) thin layer to stem cell behavior. First, the physio-mechanical properties of the ZIF8 layer grown on polydopamine (PDA) and tannic acid (TA) layers have been studied. The response of dental pulp stem cells (DPSCs) to different surface states was examined. The results showed that the uniform crystalline microstructure of the ZIF8 on PDA and TA effectively led to the 61- and 388-fold increased surface roughness, 3- and 2.5-fold moderated elastic modulus, almost 3-fold elevated surface free energy, and highly charged surfaces (ζ = -60 mV for TA/ZIF8), respectively. Beyond the inherent bioactivity of the ZIF8 layer, these substrate cues promoted advanced cell adhesion (∼two times) and high proliferation rate. Furthermore, we found a substantial increment in the differentiation efficiency of DPSCs on the ZIF8 layer, in a way that the expression of functional adipocyte (PPARG) and osteoblast (SPP1) markers was, respectively, elevated around 30 »000- and 10 »000-fold on the TA/ZIF8-coated silicon wafer (SW). Our findings support the impact of fabrication strategy on the biointerface properties of the ZIF8 layer and bring SW/TA/ZIF8 as a robust platform for managing stem cells for biomedical applications.

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http://hdl.handle.net/10453/151171