Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties.

Velu, V; Banerjee, S; Radhakrishnan, V; Gupta, G; Chellappan, DK; Kumar, N; Fuloria, S; Mehta, M; Dua, K; Malipeddi, H

Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties.

Velu, V Banerjee, S Radhakrishnan, V Gupta, G Chellappan, DK Kumar, N Fuloria, S Mehta, M Dua, K

Malipeddi, H

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Publisher:: Bentham Science Publishers Ltd.
Publication Type:: Journal Article
Citation:: Anti-inflammatory & anti-allergy agents in medicinal chemistry, 2021, 20, (3), pp. 308-315
Issue Date:: 2021-01-26

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Field	Value	Language
dc.contributor.author	Velu, V
dc.contributor.author	Banerjee, S
dc.contributor.author	Radhakrishnan, V
dc.contributor.author	Gupta, G
dc.contributor.author	Chellappan, DK
dc.contributor.author	Kumar, N
dc.contributor.author	Fuloria, S
dc.contributor.author	Mehta, M
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Malipeddi, H
dc.date.accessioned	2021-11-27T13:54:10Z
dc.date.available	2020-11-18
dc.date.available	2021-11-27T13:54:10Z
dc.date.issued	2021-01-26
dc.identifier.citation	Anti-inflammatory & anti-allergy agents in medicinal chemistry, 2021, 20, (3), pp. 308-315
dc.identifier.issn	1871-5230
dc.identifier.issn	1875-614X
dc.identifier.uri	http://hdl.handle.net/10453/151880
dc.description.abstract	<h4>Aims</h4>The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts.<h4>Materials and methods</h4>The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract.<h4>Results and discussion</h4>The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study.<h4>Conclusion</h4>These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Bentham Science Publishers Ltd.
dc.relation.ispartof	Anti-inflammatory & anti-allergy agents in medicinal chemistry
dc.relation.isbasedon	10.2174/1871523020666210126144506
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0304 Medicinal and Biomolecular Chemistry, 1107 Immunology
dc.subject.classification	Medicinal & Biomolecular Chemistry
dc.title	Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties.
dc.type	Journal Article
utslib.citation.volume	20
utslib.location.activity	United Arab Emirates
utslib.for	0304 Medicinal and Biomolecular Chemistry
utslib.for	1107 Immunology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
utslib.copyright.status	closed_access	*
dc.date.updated	2021-11-27T13:54:08Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	20
utslib.citation.issue	3

Abstract:

Aims

The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts.

Materials and methods

The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract.

Results and discussion

The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study.

Conclusion

These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

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http://hdl.handle.net/10453/151880