Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins

Remenyi, J; Hunter, CJ; Cole, C; Ando, H; Impey, S; Monk, CE; Martin, KJ; Barton, GJ; Hutvagner, G; Arthur, JSC

Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins

Remenyi, J Hunter, CJ Cole, C Ando, H Impey, S Monk, CE Martin, KJ Barton, GJ Hutvagner, G

Arthur, JSC

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Publication Type:: Journal Article
Citation:: Biochemical Journal, 2010, 428 (2), pp. 281 - 291
Issue Date:: 2010-06-01

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Field	Value	Language
dc.contributor.author	Remenyi, J	en_US
dc.contributor.author	Hunter, CJ	en_US
dc.contributor.author	Cole, C	en_US
dc.contributor.author	Ando, H	en_US
dc.contributor.author	Impey, S	en_US
dc.contributor.author	Monk, CE	en_US
dc.contributor.author	Martin, KJ	en_US
dc.contributor.author	Barton, GJ	en_US
dc.contributor.author	Hutvagner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.contributor.author	Arthur, JSC	en_US
dc.date.issued	2010-06-01	en_US
dc.identifier.citation	Biochemical Journal, 2010, 428 (2), pp. 281 - 291	en_US
dc.identifier.issn	0264-6021	en_US
dc.identifier.uri	http://hdl.handle.net/10453/15258
dc.description.abstract	Neurotrophins are growth factors that are important in neuronal development and survival as well as synapse formation and plasticity. Many of the effects of neurotrophins are mediated by changes in protein expression as a result of altered transcription or translation. To determine whether neurotrophins regulate the production of microRNAs (miRNAs), small RNA species that modulate protein translation or mRNA stability, we used deep sequencing to identify BDNF (brain-derived neurotrophic factor)-induced miRNAs in cultured primary cortical mouse neurons. This revealed that the miR-212/132 cluster contained the miRNAs most responsive to BDNF treatment. This cluster was found to produce four miRNAs: miR-132, miR-132, miR-212 and miR-212. Using specific inhibitors, mouse models and promoter analysis we have shown that the regulation of the transcription of the miR-212/132 miRNA cluster and the miRNAs derived from it are regulated by the ERK1/2 (extracellular-signal-regulated kinase 1/2) pathway, via bothMSK (mitogen and stress-activated kinase)-dependent and -independent mechanisms. © The Authors.	en_US
dc.relation.ispartof	Biochemical Journal	en_US
dc.relation.isbasedon	10.1042/BJ20100024	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Neurons	en_US
dc.subject.mesh	Cells, Cultured	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred C57BL	en_US
dc.subject.mesh	Mice, Transgenic	en_US
dc.subject.mesh	Mice, Knockout	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Benzamides	en_US
dc.subject.mesh	Mitogen-Activated Protein Kinase 1	en_US
dc.subject.mesh	Mitogen-Activated Protein Kinase 3	en_US
dc.subject.mesh	Ribosomal Protein S6 Kinases, 90-kDa	en_US
dc.subject.mesh	Brain-Derived Neurotrophic Factor	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	Blotting, Northern	en_US
dc.subject.mesh	Nucleic Acid Amplification Techniques	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Sequence Homology, Nucleic Acid	en_US
dc.subject.mesh	Phosphorylation	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Cyclic AMP Response Element-Binding Protein	en_US
dc.title	Regulation of the miR-212/132 locus by MSK1 and CREB in response to neurotrophins	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	428	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000278528800014	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	428	en_US

Abstract:

Neurotrophins are growth factors that are important in neuronal development and survival as well as synapse formation and plasticity. Many of the effects of neurotrophins are mediated by changes in protein expression as a result of altered transcription or translation. To determine whether neurotrophins regulate the production of microRNAs (miRNAs), small RNA species that modulate protein translation or mRNA stability, we used deep sequencing to identify BDNF (brain-derived neurotrophic factor)-induced miRNAs in cultured primary cortical mouse neurons. This revealed that the miR-212/132 cluster contained the miRNAs most responsive to BDNF treatment. This cluster was found to produce four miRNAs: miR-132, miR-132*, miR-212 and miR-212*. Using specific inhibitors, mouse models and promoter analysis we have shown that the regulation of the transcription of the miR-212/132 miRNA cluster and the miRNAs derived from it are regulated by the ERK1/2 (extracellular-signal-regulated kinase 1/2) pathway, via bothMSK (mitogen and stress-activated kinase)-dependent and -independent mechanisms. © The Authors.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/15258