Small RNA asymmetry in RNAi: Function in RISC assembly and gene regulation

Publication Type:
Journal Article
FEBS Letters, 2005, 579 (26), pp. 5850 - 5857
Issue Date:
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RNAi is a conserved gene-specific regulatory mechanism, which silences target gene expression transcriptionally and post-transcriptionally. The RNAi machinery converts the sequence specific information of a long double stranded RNAs (dsRNAs) into small 21-22 nt long dsRNAs (siRNAs, miRNAs) which assemble into an effector complex, the RNA induced silencing complex (RISC). RISC assembly is asymmetric; one strand of an siRNA or a miRNA preferentially incorporates into the RNA-protein complex. Here, I review the rules of the asymmetric RISC formation and discuss their possible regulatory function in several steps in RNAi. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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