Purification and Immune Phenotyping of B-1 Cells from Body Cavities of Mice.

Yenson, V; Baumgarth, N

Purification and Immune Phenotyping of B-1 Cells from Body Cavities of Mice.

Yenson, V

Baumgarth, N

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Publisher:: Springer US
Publication Type:: Journal Article
Citation:: Methods Mol Biol, 2021, 2270, pp. 27-45
Issue Date:: 2021

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Field	Value	Language
dc.contributor.author	Yenson, V https://orcid.org/0000-0002-7112-0785
dc.contributor.author	Baumgarth, N
dc.date.accessioned	2022-01-28T00:40:52Z
dc.date.available	2022-01-28T00:40:52Z
dc.date.issued	2021
dc.identifier.citation	Methods Mol Biol, 2021, 2270, pp. 27-45
dc.identifier.isbn	9781071612361
dc.identifier.issn	1064-3745
dc.identifier.issn	1940-6029
dc.identifier.uri	http://hdl.handle.net/10453/153712
dc.description.abstract	B-1 cells are fetal-origin B lymphocytes with unique developmental and functional characteristics that can generate natural, polyreactive antibodies with important functions in tissue homeostasis and immune defense. While B-1 cell frequencies in bone marrow and secondary lymphoid tissues are low, relative high frequencies exist within peritoneal and pleural cavities of mice, including both CD5+ and CD5- B-1 cells. These cells represent B-1 reservoirs that, when activated, migrate to lymphoid tissues to secrete antibodies and/or cytokines. Here, we outline efficient methods for the extraction and magnetic isolation of CD5+ B-1 cells from the peritoneal and pleural cavities as well as the separation and phenotypic characterization of CD5+ and CD5- B-1 cells by flow cytometry.
dc.format	Print
dc.language	eng
dc.publisher	Springer US
dc.relation.ispartof	Methods Mol Biol
dc.relation.isbasedon	10.1007/978-1-0716-1237-8_2
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.mesh	Animals
dc.subject.mesh	Antigens, CD
dc.subject.mesh	B-Lymphocyte Subsets
dc.subject.mesh	B-Lymphocytes
dc.subject.mesh	Bone Marrow
dc.subject.mesh	Bone Marrow Cells
dc.subject.mesh	CD5 Antigens
dc.subject.mesh	Flow Cytometry
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Peritoneal Cavity
dc.subject.mesh	Pleural Cavity
dc.subject.mesh	Peritoneal Cavity
dc.subject.mesh	Pleural Cavity
dc.subject.mesh	B-Lymphocytes
dc.subject.mesh	B-Lymphocyte Subsets
dc.subject.mesh	Bone Marrow Cells
dc.subject.mesh	Bone Marrow
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Mice
dc.subject.mesh	Antigens, CD
dc.subject.mesh	Flow Cytometry
dc.subject.mesh	CD5 Antigens
dc.title	Purification and Immune Phenotyping of B-1 Cells from Body Cavities of Mice.
dc.type	Journal Article
utslib.citation.volume	2270
utslib.location.activity	United States
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/IMPACCT
utslib.copyright.status	closed_access	*
dc.date.updated	2022-01-28T00:40:51Z
pubs.publication-status	Published
pubs.volume	2270

Abstract:

B-1 cells are fetal-origin B lymphocytes with unique developmental and functional characteristics that can generate natural, polyreactive antibodies with important functions in tissue homeostasis and immune defense. While B-1 cell frequencies in bone marrow and secondary lymphoid tissues are low, relative high frequencies exist within peritoneal and pleural cavities of mice, including both CD5+ and CD5- B-1 cells. These cells represent B-1 reservoirs that, when activated, migrate to lymphoid tissues to secrete antibodies and/or cytokines. Here, we outline efficient methods for the extraction and magnetic isolation of CD5+ B-1 cells from the peritoneal and pleural cavities as well as the separation and phenotypic characterization of CD5+ and CD5- B-1 cells by flow cytometry.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/153712