Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro

Paudel, KR; Mehta, M; Yin, GHS; Yen, LL; Malyla, V; Patel, VK; Panneerselvam, J; Madheswaran, T; MacLoughlin, R; Jha, NK; Gupta, PK; Singh, SK; Gupta, G; Kumar, P; Oliver, BG; Hansbro, PM; Chellappan, DK; Dua, K

Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro

Paudel, KR Mehta, M Yin, GHS Yen, LL Malyla, V Patel, VK Panneerselvam, J Madheswaran, T MacLoughlin, R Jha, NK Gupta, PK Singh, SK Gupta, G Kumar, P Oliver, BG Hansbro, PM Chellappan, DK Dua, K

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: Environmental Science and Pollution Research

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Field	Value	Language
dc.contributor.author	Paudel, KR
dc.contributor.author	Mehta, M
dc.contributor.author	Yin, GHS
dc.contributor.author	Yen, LL
dc.contributor.author	Malyla, V
dc.contributor.author	Patel, VK
dc.contributor.author	Panneerselvam, J
dc.contributor.author	Madheswaran, T
dc.contributor.author	MacLoughlin, R
dc.contributor.author	Jha, NK
dc.contributor.author	Gupta, PK
dc.contributor.author	Singh, SK
dc.contributor.author	Gupta, G
dc.contributor.author	Kumar, P
dc.contributor.author	Oliver, BG
dc.contributor.author	Hansbro, PM
dc.contributor.author	Chellappan, DK
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.date.accessioned	2022-02-16T13:19:06Z
dc.date.available	2022-02-16T13:19:06Z
dc.identifier.citation	Environmental Science and Pollution Research
dc.identifier.issn	0944-1344
dc.identifier.issn	1614-7499
dc.identifier.uri	http://hdl.handle.net/10453/154625
dc.description.abstract	<jats:title>Abstract</jats:title><jats:p>Non-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of cancer contributing towards 85% of all incidences of lung cancer. Berberine is an isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restricted its therapeutic applications. To overcome these limitations, we formulated berberine-loaded liquid crystalline nanoparticles (LCNs) and investigated their in vitro antiproliferative and antimigratory activity in human lung epithelial cancer cell line (A549). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), trypan blue staining, and colony forming assays were used to evaluate the anti-proliferative activity, while scratch wound healing assay and a modified Boyden chamber assay were carried out to determine the anti-migratory activity. We also investigated major proteins associated with lung cancer progression. The developed nanoparticles were found to have an average particle size of 181.3 nm with spherical shape, high entrapment efficiency (75.35%) and have shown sustained release behaviour. The most remarkable findings reported with berberine-loaded LCNs were significant suppression of proliferation, inhibition of colony formation, inhibition of invasion or migration via epithelial mesenchymal transition, and proliferation related proteins associated with cancer progression. Our findings suggest that anti-cancer compounds with the problem of poor solubility and bioavailability can be overcome by formulating them into nanotechnology-based delivery systems for better efficacy. Further in-depth investigations into anti-cancer mechanistic research will expand and strengthen the current findings of berberine-LCNs as a potential NSCLC treatment option.</jats:p>
dc.language	en
dc.publisher	Springer Science and Business Media LLC
dc.relation	Maridulu Budyari Gumal - The Sydney Partnership for Health, Education, Research and Enterprise (SPHERE)N/A
dc.relation.ispartof	Environmental Science and Pollution Research
dc.relation.isbasedon	10.1007/s11356-022-19158-2
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 05 Environmental Sciences, 06 Biological Sciences
dc.subject.classification	Environmental Sciences
dc.title	Berberine-loaded liquid crystalline nanoparticles inhibit non-small cell lung cancer proliferation and migration in vitro
dc.type	Journal Article
utslib.for	03 Chemical Sciences
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
utslib.copyright.status	closed_access	*
dc.date.updated	2022-02-16T13:19:04Z
pubs.publication-status	Published online

Abstract:

AbstractNon-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of cancer contributing towards 85% of all incidences of lung cancer. Berberine is an isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restricted its therapeutic applications. To overcome these limitations, we formulated berberine-loaded liquid crystalline nanoparticles (LCNs) and investigated their in vitro antiproliferative and antimigratory activity in human lung epithelial cancer cell line (A549). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), trypan blue staining, and colony forming assays were used to evaluate the anti-proliferative activity, while scratch wound healing assay and a modified Boyden chamber assay were carried out to determine the anti-migratory activity. We also investigated major proteins associated with lung cancer progression. The developed nanoparticles were found to have an average particle size of 181.3 nm with spherical shape, high entrapment efficiency (75.35%) and have shown sustained release behaviour. The most remarkable findings reported with berberine-loaded LCNs were significant suppression of proliferation, inhibition of colony formation, inhibition of invasion or migration via epithelial mesenchymal transition, and proliferation related proteins associated with cancer progression. Our findings suggest that anti-cancer compounds with the problem of poor solubility and bioavailability can be overcome by formulating them into nanotechnology-based delivery systems for better efficacy. Further in-depth investigations into anti-cancer mechanistic research will expand and strengthen the current findings of berberine-LCNs as a potential NSCLC treatment option.

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http://hdl.handle.net/10453/154625