Berberine-Loaded Liquid Crystalline Nanoparticles Inhibit Non-Small Cell Lung Cancer Proliferation and Migration in Vitro

Paudel, KR; Mehta, M; Yin, GHS; Yen, LL; Malyla, V; Patel, VK; Panneerselvam, J; Madheswaran, T; MacLoughlin, R; Jha, NK; Gupta, PK; Singh, SK; Gupta, G; Kumar, P; Oliver, BG; Hansbro, PM; Chellappan, DK; Dua, K

Berberine-Loaded Liquid Crystalline Nanoparticles Inhibit Non-Small Cell Lung Cancer Proliferation and Migration in Vitro

Paudel, KR Mehta, M Yin, GHS Yen, LL Malyla, V Patel, VK Panneerselvam, J Madheswaran, T MacLoughlin, R Jha, NK Gupta, PK Singh, SK Gupta, G Kumar, P Oliver, BG Hansbro, PM Chellappan, DK Dua, K

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Publisher:: Springer
Publication Type:: Journal Article
Citation:: Environmental Science and Pollution Research, 2021
Issue Date:: 2021

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Field	Value	Language
dc.contributor.author	Paudel, KR
dc.contributor.author	Mehta, M
dc.contributor.author	Yin, GHS
dc.contributor.author	Yen, LL
dc.contributor.author	Malyla, V
dc.contributor.author	Patel, VK
dc.contributor.author	Panneerselvam, J
dc.contributor.author	Madheswaran, T
dc.contributor.author	MacLoughlin, R
dc.contributor.author	Jha, NK
dc.contributor.author	Gupta, PK
dc.contributor.author	Singh, SK
dc.contributor.author	Gupta, G
dc.contributor.author	Kumar, P
dc.contributor.author	Oliver, BG
dc.contributor.author	Hansbro, PM
dc.contributor.author	Chellappan, DK
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.date.accessioned	2022-03-14T02:17:49Z
dc.date.available	2022-03-14T02:17:49Z
dc.date.issued	2021
dc.identifier.citation	Environmental Science and Pollution Research, 2021
dc.identifier.issn	0944-1344
dc.identifier.uri	http://hdl.handle.net/10453/155198
dc.description.abstract	<jats:title>Abstract</jats:title> <jats:p>Non-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of lung cancer contributing towards 85 percent of all incidences of lung cancer. Berberine is a potent isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restricted its therapeutic applications. To overcome these limitations, we synthesized Berberine-loaded liquid crystalline nanoparticles (LCNs) and investigated them <jats:italic>in vitro</jats:italic> for their antiproliferative and antimigratory properties in human lung epithelial cancer cell line (A549). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Trypan blue staining, and colony forming assays were used to evaluate the anti-proliferative activity, while scratch wound healing assay and a modified Boyden chamber assay were employed to determine the anti-migratory activity. We also investigated critical proteins associated with lung cancer. The developed nanoparticles (NPs) were found to have an average particle size of 181.3 nm with spherical shape, high entrapment efficiency (75.35 %) and have shown sustained release behaviour. The most remarkable findings reported with Berberine-loaded LCNs were significant suppression of proliferation, inhibition of colony formation, inhibition of invasion or migration <jats:italic>via</jats:italic> Epithelial Mesenchymal Transition<jats:bold> </jats:bold>(EMT) related proteins associated with cancer progression. Further in-depth investigations into anti-cancer mechanistic research will expand and strengthen the current findings of Berberine-LCNs as a potential NSCLC treatment option.</jats:p>
dc.language	en
dc.publisher	Springer
dc.relation.ispartof	Environmental Science and Pollution Research
dc.relation.isbasedon	10.21203/rs.3.rs-926601/v1
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	03 Chemical Sciences, 05 Environmental Sciences, 06 Biological Sciences
dc.subject.classification	Environmental Sciences
dc.title	Berberine-Loaded Liquid Crystalline Nanoparticles Inhibit Non-Small Cell Lung Cancer Proliferation and Migration in Vitro
dc.type	Journal Article
utslib.for	03 Chemical Sciences
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-03-14T02:17:47Z
pubs.publication-status	Accepted

Abstract:

Abstract Non-small cell lung cancer (NSCLC) is reported to have a high incidence rate and is one of the most prevalent types of lung cancer contributing towards 85 percent of all incidences of lung cancer. Berberine is a potent isoquinoline alkaloid which offers a broad range of therapeutical and pharmacological actions against cancer. However, extremely low water solubility and poor oral bioavailability have largely restricted its therapeutic applications. To overcome these limitations, we synthesized Berberine-loaded liquid crystalline nanoparticles (LCNs) and investigated them in vitro for their antiproliferative and antimigratory properties in human lung epithelial cancer cell line (A549). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Trypan blue staining, and colony forming assays were used to evaluate the anti-proliferative activity, while scratch wound healing assay and a modified Boyden chamber assay were employed to determine the anti-migratory activity. We also investigated critical proteins associated with lung cancer. The developed nanoparticles (NPs) were found to have an average particle size of 181.3 nm with spherical shape, high entrapment efficiency (75.35 %) and have shown sustained release behaviour. The most remarkable findings reported with Berberine-loaded LCNs were significant suppression of proliferation, inhibition of colony formation, inhibition of invasion or migration via Epithelial Mesenchymal Transition (EMT) related proteins associated with cancer progression. Further in-depth investigations into anti-cancer mechanistic research will expand and strengthen the current findings of Berberine-LCNs as a potential NSCLC treatment option.

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http://hdl.handle.net/10453/155198