The impact and fate of clarithromycin in anaerobic digestion of waste activated sludge for biogas production.

Zeng, S; Sun, J; Chen, Z; Xu, Q; Wei, W; Wang, D; Ni, B-J

The impact and fate of clarithromycin in anaerobic digestion of waste activated sludge for biogas production.

Zeng, S Sun, J Chen, Z Xu, Q Wei, W

Wang, D Ni, B-J

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Environmental Research, 2021, 195, pp. 1-10
Issue Date:: 2021-04

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Field	Value	Language
dc.contributor.author	Zeng, S
dc.contributor.author	Sun, J
dc.contributor.author	Chen, Z
dc.contributor.author	Xu, Q
dc.contributor.author	Wei, W https://orcid.org/0000-0001-5613-337X
dc.contributor.author	Wang, D
dc.contributor.author	Ni, B-J
dc.date.accessioned	2022-05-12T05:33:34Z
dc.date.available	2021-01-13
dc.date.available	2022-05-12T05:33:34Z
dc.date.issued	2021-04
dc.identifier.citation	Environmental Research, 2021, 195, pp. 1-10
dc.identifier.issn	0013-9351
dc.identifier.issn	1096-0953
dc.identifier.uri	http://hdl.handle.net/10453/157284
dc.description.abstract	Clarithromycin retained in waste activated sludge (WAS) inevitably enters the anaerobic digestion system. So far, the complex impacts and fate of clarithromycin in continuous operated WAS anaerobic digestion system are still unclear. In this study, two semi-continuous long-term reactors were set up to investigate the effect of clarithromycin on biogas production and antibiotic resistance genes (ARGs) during WAS anaerobic digestion, and a batch test was carried out to explore the potential metabolic mechanism. Experimental results showed that clarithromycin at lower concentrations (i.e., 0.1 and 1.0 mg/L) did not affect biogas production, whereas the decrease in biogas production was observed when the concentration of clarithromycin was further increased to 10 mg/L. Correspondingly, the relative abundance of functional bacteria in WAS anaerobic digestion (i.e., Anaerolineaceae and Microtrichales) was reduced with long-term clarithromycin exposure. The investigation of ARGs suggested that the effect of methylation belonging to the target site modification played a critical role for the anaerobic microorganisms in the expression of antibiotic resistance, and ermF, played dominated ARGs, presented the most remarkable proliferation. In comparison, the role of efflux pump was weakened with a significant decrease of two detected efflux genes. During WAS anaerobic digestion, clarithromycin could be partially degraded into metabolites with lower antimicrobial activity including oleandomycin and 5-O-desosaminyl-6-O-methylerythronolide and other metabolites without antimicrobial activity.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	Environmental Research
dc.relation.isbasedon	10.1016/j.envres.2021.110792
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 05 Environmental Sciences, 06 Biological Sciences
dc.subject.classification	Toxicology
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Biofuels
dc.subject.mesh	Bioreactors
dc.subject.mesh	Clarithromycin
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Sewage
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Biofuels
dc.subject.mesh	Bioreactors
dc.subject.mesh	Clarithromycin
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Sewage
dc.subject.mesh	Clarithromycin
dc.subject.mesh	Bioreactors
dc.subject.mesh	Sewage
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Biofuels
dc.title	The impact and fate of clarithromycin in anaerobic digestion of waste activated sludge for biogas production.
dc.type	Journal Article
utslib.citation.volume	195
utslib.location.activity	Netherlands
utslib.for	03 Chemical Sciences
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-05-12T05:33:30Z
pubs.publication-status	Published
pubs.volume	195

Abstract:

Clarithromycin retained in waste activated sludge (WAS) inevitably enters the anaerobic digestion system. So far, the complex impacts and fate of clarithromycin in continuous operated WAS anaerobic digestion system are still unclear. In this study, two semi-continuous long-term reactors were set up to investigate the effect of clarithromycin on biogas production and antibiotic resistance genes (ARGs) during WAS anaerobic digestion, and a batch test was carried out to explore the potential metabolic mechanism. Experimental results showed that clarithromycin at lower concentrations (i.e., 0.1 and 1.0 mg/L) did not affect biogas production, whereas the decrease in biogas production was observed when the concentration of clarithromycin was further increased to 10 mg/L. Correspondingly, the relative abundance of functional bacteria in WAS anaerobic digestion (i.e., Anaerolineaceae and Microtrichales) was reduced with long-term clarithromycin exposure. The investigation of ARGs suggested that the effect of methylation belonging to the target site modification played a critical role for the anaerobic microorganisms in the expression of antibiotic resistance, and ermF, played dominated ARGs, presented the most remarkable proliferation. In comparison, the role of efflux pump was weakened with a significant decrease of two detected efflux genes. During WAS anaerobic digestion, clarithromycin could be partially degraded into metabolites with lower antimicrobial activity including oleandomycin and 5-O-desosaminyl-6-O-methylerythronolide and other metabolites without antimicrobial activity.

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http://hdl.handle.net/10453/157284