The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production

Hossain, N; Hoong, LL; Barua, P; Soudagar, MEM; Mahlia, TMI

The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production

Hossain, N Hoong, LL Barua, P Soudagar, MEM Mahlia, TMI

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Publisher:: Springer
Publication Type:: Journal Article
Citation:: Korean Journal of Chemical Engineering, 2021, 38, (12), pp. 2493-2499
Issue Date:: 2021-12-01

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Field	Value	Language
dc.contributor.author	Hossain, N
dc.contributor.author	Hoong, LL
dc.contributor.author	Barua, P
dc.contributor.author	Soudagar, MEM
dc.contributor.author	Mahlia, TMI
dc.date.accessioned	2022-05-16T01:26:16Z
dc.date.available	2022-05-16T01:26:16Z
dc.date.issued	2021-12-01
dc.identifier.citation	Korean Journal of Chemical Engineering, 2021, 38, (12), pp. 2493-2499
dc.identifier.issn	0256-1115
dc.identifier.issn	1975-7220
dc.identifier.uri	http://hdl.handle.net/10453/157402
dc.description.abstract	Enzymatic hydrolysis of waste biomass for bioethanol production is considered a decades old traditional, inexpensive, and energy-effective approach. In this study, waste office paper was pretreated with diluted sulfuric acid (H2SO4) and hydrolyzed with one of the most available and cost-effective enzymes, cellulase derived from Trichoderma reesei, under submerged static condition. Three different pretreatment approaches—cut into 2 cm2, blended with distilled water, and pretreated with diluted H2SO4—have been implemented, and pretreatment with diluted H2SO4 was the most effective. Hydrolysis with different concentrations—0.5 M, 1.0 M, 1.5 M, 2.0 M of H2SO4—was performed. The maximum glucose content was obtained at 2.0 M H2SO4 at 90 min reaction time, and glucose yield was 0.11 g glucose/g wastepaper. The cut paper, wet-blended, and acid-treated wastepaper was hydrolyzed with cellulase enzyme for 2, 4, and 5 consecutive days with 5 mg, 10 mg, 15 mg, and 20 mg enzyme loadings. The maximum glucose content obtained was 9.75 g/l from acid-treated wastepaper, after 5 days of enzymatic hydrolysis with 20 mg enzyme loading and a glucose yield of a 0.5 g glucose/g wastepaper. The wastepaper hydrolysate was further fermented for 6, 8, and 10 hours continuously with Saccharomyces cerevisiae (yeast), and at 10 hours of fermentation, the maximum glucose consumption was 0.18 g by yeast. Further, HPLC analysis of the fermented medium presented a strong peak of bioethanol content at 16.12 min. The distillation of bioethanol by rotary evaporator presented 0.79 ml bioethanol/fermented solution, which indicated the conversion efficiency of 79%.
dc.language	en
dc.publisher	Springer
dc.relation.ispartof	Korean Journal of Chemical Engineering
dc.relation.isbasedon	10.1007/s11814-021-0914-1
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0904 Chemical Engineering, 0913 Mechanical Engineering
dc.subject.classification	Chemical Engineering
dc.title	The effect of enzymatic hydrolysis of pretreated wastepaper for bioethanol production
dc.type	Journal Article
utslib.citation.volume	38
utslib.for	0904 Chemical Engineering
utslib.for	0913 Mechanical Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CAMGIS - Centre for Advanced Modelling and Geospatial lnformation Systems
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-05-16T01:26:15Z
pubs.issue	12
pubs.publication-status	Published
pubs.volume	38
utslib.citation.issue	12

Abstract:

Enzymatic hydrolysis of waste biomass for bioethanol production is considered a decades old traditional, inexpensive, and energy-effective approach. In this study, waste office paper was pretreated with diluted sulfuric acid (H2SO4) and hydrolyzed with one of the most available and cost-effective enzymes, cellulase derived from Trichoderma reesei, under submerged static condition. Three different pretreatment approaches—cut into 2 cm2, blended with distilled water, and pretreated with diluted H2SO4—have been implemented, and pretreatment with diluted H2SO4 was the most effective. Hydrolysis with different concentrations—0.5 M, 1.0 M, 1.5 M, 2.0 M of H2SO4—was performed. The maximum glucose content was obtained at 2.0 M H2SO4 at 90 min reaction time, and glucose yield was 0.11 g glucose/g wastepaper. The cut paper, wet-blended, and acid-treated wastepaper was hydrolyzed with cellulase enzyme for 2, 4, and 5 consecutive days with 5 mg, 10 mg, 15 mg, and 20 mg enzyme loadings. The maximum glucose content obtained was 9.75 g/l from acid-treated wastepaper, after 5 days of enzymatic hydrolysis with 20 mg enzyme loading and a glucose yield of a 0.5 g glucose/g wastepaper. The wastepaper hydrolysate was further fermented for 6, 8, and 10 hours continuously with Saccharomyces cerevisiae (yeast), and at 10 hours of fermentation, the maximum glucose consumption was 0.18 g by yeast. Further, HPLC analysis of the fermented medium presented a strong peak of bioethanol content at 16.12 min. The distillation of bioethanol by rotary evaporator presented 0.79 ml bioethanol/fermented solution, which indicated the conversion efficiency of 79%.

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http://hdl.handle.net/10453/157402