Propidium iodide enabled live imaging of Pasteuria sp.-Pratylenchus zeae infection studies under fluorescence microscopy.

Perrine-Walker, F; Le, K

Propidium iodide enabled live imaging of Pasteuria sp.-Pratylenchus zeae infection studies under fluorescence microscopy.

Perrine-Walker, F Le, K

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Publisher:: Springer
Publication Type:: Journal Article
Citation:: Protoplasma: an international journal of cell biology, 2021, 258, (2), pp. 279-287
Issue Date:: 2021-03

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Field	Value	Language
dc.contributor.author	Perrine-Walker, F
dc.contributor.author	Le, K
dc.date.accessioned	2022-05-20T01:09:59Z
dc.date.available	2020-10-07
dc.date.available	2022-05-20T01:09:59Z
dc.date.issued	2021-03
dc.identifier.citation	Protoplasma: an international journal of cell biology, 2021, 258, (2), pp. 279-287
dc.identifier.issn	0033-183X
dc.identifier.issn	1615-6102
dc.identifier.uri	http://hdl.handle.net/10453/157546
dc.description.abstract	Live imaging allows observations of cell structures and processes in real time, to monitor dynamic changes within living organisms compared to fixed organisms. Fluorescence microscopy was used to monitor the dynamic infection process of the nematode parasitic bacterium Pasteuria sp. and the sugarcane root-lesion nematode, Pratylenchus zeae. Under fluorescence microscopy, green-autofluorescent globules were observed in live control and Pasteuria sp.-infected nematodes. Only nematodes killed by Pasteuria sp. or heat treated displayed a diffuse pattern of autofluorescence. Propidium iodide (PI), used as a cell membrane integrity indicator, confirmed that the nematode's cuticle acts as an impermeable barrier. PI stained cells/DNA of heat-treated control and Pasteuria sp.-infected P. zeae. PI as a counterstain facilitated the location of Pasteuria endospores on the cuticle surface of P. zeae. No PI staining was observed in sporangia and in endospores within the nematode body. However, PI specifically stained endospores on the cuticle surface and within the cuticle carcass showing, in mature propagules, a ring-like pattern. Live imaging, combined with fluorescence microscopy and fluorescent dyes such as PI, appears useful in live studies on plant nematode interactions with nematophagous bacteria.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Springer
dc.relation.ispartof	Protoplasma: an international journal of cell biology
dc.relation.isbasedon	10.1007/s00709-020-01567-0
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0601 Biochemistry and Cell Biology, 0607 Plant Biology
dc.subject.classification	Plant Biology & Botany
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Pasteuria
dc.subject.mesh	Propidium
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Pasteuria
dc.subject.mesh	Propidium
dc.subject.mesh	Propidium
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Pasteuria
dc.title	Propidium iodide enabled live imaging of Pasteuria sp.-Pratylenchus zeae infection studies under fluorescence microscopy.
dc.type	Journal Article
utslib.citation.volume	258
utslib.location.activity	Austria
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0607 Plant Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-05-20T01:09:57Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	258
utslib.citation.issue	2

Abstract:

Live imaging allows observations of cell structures and processes in real time, to monitor dynamic changes within living organisms compared to fixed organisms. Fluorescence microscopy was used to monitor the dynamic infection process of the nematode parasitic bacterium Pasteuria sp. and the sugarcane root-lesion nematode, Pratylenchus zeae. Under fluorescence microscopy, green-autofluorescent globules were observed in live control and Pasteuria sp.-infected nematodes. Only nematodes killed by Pasteuria sp. or heat treated displayed a diffuse pattern of autofluorescence. Propidium iodide (PI), used as a cell membrane integrity indicator, confirmed that the nematode's cuticle acts as an impermeable barrier. PI stained cells/DNA of heat-treated control and Pasteuria sp.-infected P. zeae. PI as a counterstain facilitated the location of Pasteuria endospores on the cuticle surface of P. zeae. No PI staining was observed in sporangia and in endospores within the nematode body. However, PI specifically stained endospores on the cuticle surface and within the cuticle carcass showing, in mature propagules, a ring-like pattern. Live imaging, combined with fluorescence microscopy and fluorescent dyes such as PI, appears useful in live studies on plant nematode interactions with nematophagous bacteria.

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http://hdl.handle.net/10453/157546