Use of calcofluor white to study the trapping of Pratylenchus coffeae by nematophagous fungi Arthrobotrys musiformis and Arthrobotrys oligospora

Perrine-Walker, FM

Use of calcofluor white to study the trapping of Pratylenchus coffeae by nematophagous fungi Arthrobotrys musiformis and Arthrobotrys oligospora

Perrine-Walker, FM

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Publisher:: Springer
Publication Type:: Journal Article
Citation:: Australasian Plant Pathology, 2021, 50, (3), pp. 357-364
Issue Date:: 2021-01-01

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Field	Value	Language
dc.contributor.author	Perrine-Walker, FM
dc.date.accessioned	2022-06-12T22:07:22Z
dc.date.available	2022-06-12T22:07:22Z
dc.date.issued	2021-01-01
dc.identifier.citation	Australasian Plant Pathology, 2021, 50, (3), pp. 357-364
dc.identifier.issn	0815-3191
dc.identifier.issn	1448-6032
dc.identifier.uri	http://hdl.handle.net/10453/158128
dc.description.abstract	Pratylenchus coffeae is a migratory endoparasite of the root cortex and corm of Musa species and causes root lesions in coffee, sugarcane and citrus. Pratylenchus coffeae from diseased banana roots from Queensland were maintained in carrot jars for in vitro infection studies by nematophagous fungi. The aim of this work was to use a β-glucan specific fluorescent stain, calcofluor white, to study P. coffeae being trapped by two nematophagous fungal isolates Arthrobotrys musiformis and Arthrobotrys oligospora in vitro and determine whether the stain showed features of the nematode-fungal interaction that are not apparent using other observation techniques. These in vitro studies confirmed that when nematodes were stained after being trapped by these fungi, hyphae could be seen originating from the trapping device and growing within the bodies of the trapped nematodes. In addition, a putative fluorescence signal with calcofluor white was detected in the stylet and the pharyngeal region of P. coffeae suggesting the presence of β-glucan-like compound in these sites.
dc.language	English
dc.publisher	Springer
dc.relation.ispartof	Australasian Plant Pathology
dc.relation.isbasedon	10.1007/s13313-021-00781-5
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0605 Microbiology, 0607 Plant Biology, 0703 Crop and Pasture Production
dc.subject.classification	Plant Biology & Botany
dc.title	Use of calcofluor white to study the trapping of Pratylenchus coffeae by nematophagous fungi Arthrobotrys musiformis and Arthrobotrys oligospora
dc.type	Journal Article
utslib.citation.volume	50
utslib.for	0605 Microbiology
utslib.for	0607 Plant Biology
utslib.for	0703 Crop and Pasture Production
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-06-12T22:07:21Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	50
utslib.citation.issue	3

Abstract:

Pratylenchus coffeae is a migratory endoparasite of the root cortex and corm of Musa species and causes root lesions in coffee, sugarcane and citrus. Pratylenchus coffeae from diseased banana roots from Queensland were maintained in carrot jars for in vitro infection studies by nematophagous fungi. The aim of this work was to use a β-glucan specific fluorescent stain, calcofluor white, to study P. coffeae being trapped by two nematophagous fungal isolates Arthrobotrys musiformis and Arthrobotrys oligospora in vitro and determine whether the stain showed features of the nematode-fungal interaction that are not apparent using other observation techniques. These in vitro studies confirmed that when nematodes were stained after being trapped by these fungi, hyphae could be seen originating from the trapping device and growing within the bodies of the trapped nematodes. In addition, a putative fluorescence signal with calcofluor white was detected in the stylet and the pharyngeal region of P. coffeae suggesting the presence of β-glucan-like compound in these sites.

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http://hdl.handle.net/10453/158128