Formulation, Characterisation and In vitro Cytotoxic Effect of Lens culinaris Medikus Seeds Extract Loaded Chitosan Microspheres.

Vohra, K; Mehta, M; Garg, V; Dua, K; Dureja, H

Formulation, Characterisation and In vitro Cytotoxic Effect of Lens culinaris Medikus Seeds Extract Loaded Chitosan Microspheres.

Vohra, K Mehta, M Garg, V Dua, K

Dureja, H

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Publisher:: Bentham Science Publishers
Publication Type:: Journal Article
Citation:: Current Molecular Pharmacology, 2021, 14, (3), pp. 448-457
Issue Date:: 2021

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Field	Value	Language
dc.contributor.author	Vohra, K
dc.contributor.author	Mehta, M
dc.contributor.author	Garg, V
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Dureja, H
dc.date.accessioned	2022-06-21T22:50:21Z
dc.date.available	2020-11-12
dc.date.available	2022-06-21T22:50:21Z
dc.date.issued	2021
dc.identifier.citation	Current Molecular Pharmacology, 2021, 14, (3), pp. 448-457
dc.identifier.issn	1874-4672
dc.identifier.issn	1874-4702
dc.identifier.uri	http://hdl.handle.net/10453/158300
dc.description.abstract	OBJECTIVE: The aim of present study was to formulate chitosan microspheres loaded with ethanolic extract of Lens culinaris Medikus (L.culinaris) seeds (ME) and to explore its anticancer potential against lung cancer (A549) cell line. METHODS: Central composite design was applied to prepare and optimise the chitosan microspheres. The prepared microspheres were evaluated for its physicochemical characterisation, in vitro drug release and anti-cancer potential in vitro. RESULTS: L.culinaris loaded chitosan microspheres were prepared successfully with suitable particle size, entrapment efficiency and drug release. The developed ME were spherical shaped with the particle size of 2.08 μm. The drug entrapment efficiency and cumulative drug release was found 1.58±0.02% and 81.95±0.35%, respectively. Differential scanning calorimetry studies revealed no interaction between drugs and polymers used. The cytotoxic effect of the optimised formulation revealed a significant response as compared to the ethanolic extract of L.culinaris seeds (IC50: 22.56 μg/ml vs. 63.58 μg/ml), which was comparable to that of reference drug, doxorubicin (22 μg/ml). These observations demonstrate that the optimised microspheres are effective against lung cancer (A549) cells. CONCLUSION: The significant cytotoxic response of the developed microspheres may be attributed due to its low particle size, high entrapment efficiency and prolonged drug release profile.
dc.format	Print
dc.language	eng
dc.publisher	Bentham Science Publishers
dc.relation.ispartof	Current Molecular Pharmacology
dc.relation.isbasedon	10.2174/1874467214666210210124739
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Chitosan
dc.subject.mesh	Lens Plant
dc.subject.mesh	Microspheres
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Chitosan
dc.subject.mesh	Lens Plant
dc.subject.mesh	Microspheres
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Lens Plant
dc.subject.mesh	Chitosan
dc.subject.mesh	Plant Extracts
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Microspheres
dc.title	Formulation, Characterisation and In vitro Cytotoxic Effect of Lens culinaris Medikus Seeds Extract Loaded Chitosan Microspheres.
dc.type	Journal Article
utslib.citation.volume	14
utslib.location.activity	United Arab Emirates
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-06-21T22:50:19Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	14
utslib.citation.issue	3

Abstract:

OBJECTIVE: The aim of present study was to formulate chitosan microspheres loaded with ethanolic extract of Lens culinaris Medikus (L.culinaris) seeds (ME) and to explore its anticancer potential against lung cancer (A549) cell line. METHODS: Central composite design was applied to prepare and optimise the chitosan microspheres. The prepared microspheres were evaluated for its physicochemical characterisation, in vitro drug release and anti-cancer potential in vitro. RESULTS: L.culinaris loaded chitosan microspheres were prepared successfully with suitable particle size, entrapment efficiency and drug release. The developed ME were spherical shaped with the particle size of 2.08 μm. The drug entrapment efficiency and cumulative drug release was found 1.58±0.02% and 81.95±0.35%, respectively. Differential scanning calorimetry studies revealed no interaction between drugs and polymers used. The cytotoxic effect of the optimised formulation revealed a significant response as compared to the ethanolic extract of L.culinaris seeds (IC50: 22.56 μg/ml vs. 63.58 μg/ml), which was comparable to that of reference drug, doxorubicin (22 μg/ml). These observations demonstrate that the optimised microspheres are effective against lung cancer (A549) cells. CONCLUSION: The significant cytotoxic response of the developed microspheres may be attributed due to its low particle size, high entrapment efficiency and prolonged drug release profile.

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http://hdl.handle.net/10453/158300