Optimization Studies on Imatinib Mesylate Loaded Nanoliposomes Using Box-Behnken Design

Dahiya, M; Awasthi, R; Gupta, G; Singh, SK; Gulati, M; Jha, NK; Jha, SK; Sharma, A; Prasher, P; Anand, K; Chellappan, DK; Dua, K; Dureja, H

Optimization Studies on Imatinib Mesylate Loaded Nanoliposomes Using Box-Behnken Design

Dahiya, M Awasthi, R Gupta, G Singh, SK Gulati, M Jha, NK Jha, SK Sharma, A Prasher, P Anand, K Chellappan, DK Dua, K

Dureja, H

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Publisher:: Open-Access House of Science and Technology
Publication Type:: Journal Article
Citation:: Nano Biomedicine and Engineering, 2022, 14, (1), pp. 23-37
Issue Date:: 2022-01-01

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Field	Value	Language
dc.contributor.author	Dahiya, M
dc.contributor.author	Awasthi, R
dc.contributor.author	Gupta, G
dc.contributor.author	Singh, SK
dc.contributor.author	Gulati, M
dc.contributor.author	Jha, NK
dc.contributor.author	Jha, SK
dc.contributor.author	Sharma, A
dc.contributor.author	Prasher, P
dc.contributor.author	Anand, K
dc.contributor.author	Chellappan, DK
dc.contributor.author	Dua, K https://orcid.org/0000-0002-7507-1159
dc.contributor.author	Dureja, H
dc.date.accessioned	2022-11-22T00:49:09Z
dc.date.available	2022-11-22T00:49:09Z
dc.date.issued	2022-01-01
dc.identifier.citation	Nano Biomedicine and Engineering, 2022, 14, (1), pp. 23-37
dc.identifier.issn	2150-5578
dc.identifier.issn	2150-5578
dc.identifier.uri	http://hdl.handle.net/10453/163635
dc.description.abstract	Nanoliposomes are bilayer phospholipid vesicles used to encapsulate and deliver therapeutic agents. The study was aimed to investigate the effects of critical variables on nanoliposomes characteristics. Imatinib mesylate-loaded nanoliposomes were formulated by the two-step emulsification process using a high-speed homogenizer system and probe-type ultrasonicator. The Box-Behnken design was utilized to optimize the process parameters. The mean particle size of nanoliposomes was found to be 211 nm to 623.3 nm with a low value of polydispersity index (0.005 to 0.7). Zeta potential values varied from -27.6 mV to -9.2 mV in uncoated nanoliposomes to +27.5 mV in chitosancoated nanoliposomes. The encapsulation efficiency in formulation NLP-H8 containing 200 mg of phosphatidylcholine, homogenization speed of 12000 rpm, and 7 min of sonication time was found to be 76.49% without the coating and 85.4% in 0.2% w/v chitosan-coated nanoliposomes. TEM image confirmed the spherical shape of nanoliposomes. In-vitro drug release study demonstrated that the optimized nanoliposomal formulations released 84.67% of the loaded drug after 24 h in 0.1 N HCl. The IC50 value of formulation NLP-H8 was found to be 7.98 μM. Nanoliposomal formulations were prepared successfully with suitable size, morphology, encapsulation efficiency, and drug release. The models developed in this study may be utilized further as a response surface for the various parameters of nanoliposomes.
dc.language	en
dc.publisher	Open-Access House of Science and Technology
dc.relation.ispartof	Nano Biomedicine and Engineering
dc.relation.isbasedon	10.5101/nbe.v14i1.p23-37
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0903 Biomedical Engineering, 1007 Nanotechnology, 1103 Clinical Sciences
dc.title	Optimization Studies on Imatinib Mesylate Loaded Nanoliposomes Using Box-Behnken Design
dc.type	Journal Article
utslib.citation.volume	14
utslib.for	0903 Biomedical Engineering
utslib.for	1007 Nanotechnology
utslib.for	1103 Clinical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Public Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Graduate School of Health/GSH.Pharmacy
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-11-22T00:49:08Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	14
utslib.citation.issue	1

Abstract:

Nanoliposomes are bilayer phospholipid vesicles used to encapsulate and deliver therapeutic agents. The study was aimed to investigate the effects of critical variables on nanoliposomes characteristics. Imatinib mesylate-loaded nanoliposomes were formulated by the two-step emulsification process using a high-speed homogenizer system and probe-type ultrasonicator. The Box-Behnken design was utilized to optimize the process parameters. The mean particle size of nanoliposomes was found to be 211 nm to 623.3 nm with a low value of polydispersity index (0.005 to 0.7). Zeta potential values varied from -27.6 mV to -9.2 mV in uncoated nanoliposomes to +27.5 mV in chitosancoated nanoliposomes. The encapsulation efficiency in formulation NLP-H8 containing 200 mg of phosphatidylcholine, homogenization speed of 12000 rpm, and 7 min of sonication time was found to be 76.49% without the coating and 85.4% in 0.2% w/v chitosan-coated nanoliposomes. TEM image confirmed the spherical shape of nanoliposomes. In-vitro drug release study demonstrated that the optimized nanoliposomal formulations released 84.67% of the loaded drug after 24 h in 0.1 N HCl. The IC50 value of formulation NLP-H8 was found to be 7.98 μM. Nanoliposomal formulations were prepared successfully with suitable size, morphology, encapsulation efficiency, and drug release. The models developed in this study may be utilized further as a response surface for the various parameters of nanoliposomes.

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http://hdl.handle.net/10453/163635