Pharmacological evaluation of bromelain in mouse model of Alzheimer's disease.

Kumar, R; Kumar, R; Sharma, N; Khurana, N; Singh, SK; Satija, S; Mehta, M; Vyas, M

Pharmacological evaluation of bromelain in mouse model of Alzheimer's disease.

Kumar, R Kumar, R Sharma, N Khurana, N Singh, SK Satija, S Mehta, M Vyas, M

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: NeuroToxicology, 2022, 90, pp. 19-34
Issue Date:: 2022-05

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Field	Value	Language
dc.contributor.author	Kumar, R
dc.contributor.author	Kumar, R
dc.contributor.author	Sharma, N
dc.contributor.author	Khurana, N
dc.contributor.author	Singh, SK
dc.contributor.author	Satija, S
dc.contributor.author	Mehta, M
dc.contributor.author	Vyas, M
dc.date.accessioned	2022-11-23T01:49:09Z
dc.date.available	2022-02-21
dc.date.available	2022-11-23T01:49:09Z
dc.date.issued	2022-05
dc.identifier.citation	NeuroToxicology, 2022, 90, pp. 19-34
dc.identifier.issn	0161-813X
dc.identifier.issn	1872-9711
dc.identifier.uri	http://hdl.handle.net/10453/163664
dc.description.abstract	The current study elucidates pharmacological evaluation of bromelain as a bioactive compound obtain from pineapple stem belongs to family Bromeliaceae in AlCl3 and D - galactose induced mice. In mice, co-administration of AlCl3 at dose 5 mg/kg b.w., via the oral route, and D - galactose at dose 60 mg/kg b.w., via intraperitoneal route for 90 days resulted in cognitive impairment, spatial learning, and memory deficits, as well as neurotoxicity. However, 30 consecutive days, treatments via an intraperitoneal route with bromelain low dose (Brm L) at dose 10 mg/kg b.w., bromelain high dose (Brm H) at dose 20 mg/kg b.w., donepezil (Dnpz) at dose 2 mg/kg b.w., and Brm L + Dnpz at doses 10, 2 mg/kg b.w. were considerably reversed the effect of AlCl3 and D - galactose induced AD mice. Consequences of behavioral parameters (Morris water maze, elevated plus maze and locomotor), biochemical estimation (MDA, GSH, SOD, CAT, Nitrite and AChE), and ELISA tests (mouse BACE, Aβ1 - 42, TNF-α, IL-6, and BDNF) confirmed significant (p < 0.05) neuroprotective effect of treatments in AlCl3 and D - galactose induced mice. Additionally, hematoxylin and eosin staining of the cerebral cortex and the hippocampus exposed eosinophilic lesions and hyperchromatic nuclei in AD mice, but these neurodegenerative effects were eliminated by Brm L, Brm H, Dnpz, and Brm L + Dnpz treatments. Thus, bromelain alone and in combination with donepezil prevent AlCl3 and D - galactose induced spatial learning and memory deficits, as well as cognitive impairment, by increasing cholinergic activity and synaptic plasticity, as well as reducing oxidative damage, neuroinflammation, Aβ 1-42 aggregations, and histopathological damage, according to our findings. The present study consequences indicate that bromelain alone and in combination with donepezil appears to have neuroprotective properties. Henceforward, this may be a promising treatment option for Alzheimer's disease.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation.ispartof	NeuroToxicology
dc.relation.isbasedon	10.1016/j.neuro.2022.02.009
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1109 Neurosciences, 1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Toxicology
dc.subject.mesh	Aluminum Chloride
dc.subject.mesh	Alzheimer Disease
dc.subject.mesh	Animals
dc.subject.mesh	Bromelains
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Donepezil
dc.subject.mesh	Galactose
dc.subject.mesh	Hippocampus
dc.subject.mesh	Maze Learning
dc.subject.mesh	Memory Disorders
dc.subject.mesh	Mice
dc.subject.mesh	Neuroprotective Agents
dc.subject.mesh	Oxidative Stress
dc.subject.mesh	Aluminum Chloride
dc.subject.mesh	Alzheimer Disease
dc.subject.mesh	Animals
dc.subject.mesh	Bromelains
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Donepezil
dc.subject.mesh	Galactose
dc.subject.mesh	Hippocampus
dc.subject.mesh	Maze Learning
dc.subject.mesh	Memory Disorders
dc.subject.mesh	Mice
dc.subject.mesh	Neuroprotective Agents
dc.subject.mesh	Oxidative Stress
dc.subject.mesh	Hippocampus
dc.subject.mesh	Animals
dc.subject.mesh	Mice
dc.subject.mesh	Alzheimer Disease
dc.subject.mesh	Memory Disorders
dc.subject.mesh	Disease Models, Animal
dc.subject.mesh	Bromelains
dc.subject.mesh	Galactose
dc.subject.mesh	Neuroprotective Agents
dc.subject.mesh	Maze Learning
dc.subject.mesh	Oxidative Stress
dc.subject.mesh	Donepezil
dc.subject.mesh	Aluminum Chloride
dc.title	Pharmacological evaluation of bromelain in mouse model of Alzheimer's disease.
dc.type	Journal Article
utslib.citation.volume	90
utslib.location.activity	Netherlands
utslib.for	1109 Neurosciences
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/Public Health
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-11-23T01:49:06Z
pubs.publication-status	Published
pubs.volume	90

Abstract:

The current study elucidates pharmacological evaluation of bromelain as a bioactive compound obtain from pineapple stem belongs to family Bromeliaceae in AlCl3 and D - galactose induced mice. In mice, co-administration of AlCl3 at dose 5 mg/kg b.w., via the oral route, and D - galactose at dose 60 mg/kg b.w., via intraperitoneal route for 90 days resulted in cognitive impairment, spatial learning, and memory deficits, as well as neurotoxicity. However, 30 consecutive days, treatments via an intraperitoneal route with bromelain low dose (Brm L) at dose 10 mg/kg b.w., bromelain high dose (Brm H) at dose 20 mg/kg b.w., donepezil (Dnpz) at dose 2 mg/kg b.w., and Brm L + Dnpz at doses 10, 2 mg/kg b.w. were considerably reversed the effect of AlCl3 and D - galactose induced AD mice. Consequences of behavioral parameters (Morris water maze, elevated plus maze and locomotor), biochemical estimation (MDA, GSH, SOD, CAT, Nitrite and AChE), and ELISA tests (mouse BACE, Aβ1 - 42, TNF-α, IL-6, and BDNF) confirmed significant (p < 0.05) neuroprotective effect of treatments in AlCl3 and D - galactose induced mice. Additionally, hematoxylin and eosin staining of the cerebral cortex and the hippocampus exposed eosinophilic lesions and hyperchromatic nuclei in AD mice, but these neurodegenerative effects were eliminated by Brm L, Brm H, Dnpz, and Brm L + Dnpz treatments. Thus, bromelain alone and in combination with donepezil prevent AlCl3 and D - galactose induced spatial learning and memory deficits, as well as cognitive impairment, by increasing cholinergic activity and synaptic plasticity, as well as reducing oxidative damage, neuroinflammation, Aβ 1-42 aggregations, and histopathological damage, according to our findings. The present study consequences indicate that bromelain alone and in combination with donepezil appears to have neuroprotective properties. Henceforward, this may be a promising treatment option for Alzheimer's disease.

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http://hdl.handle.net/10453/163664