A novel sulfide-driven denitrification methane oxidation (SDMO) system: Operational performance and metabolic mechanisms.

Wang, W; Zhao, L; Ni, B-J; Yin, T-M; Zhang, R-C; Yu, M; Shao, B; Xu, X-J; Xing, D-F; Lee, D-J; Ren, N-Q; Chen, C

A novel sulfide-driven denitrification methane oxidation (SDMO) system: Operational performance and metabolic mechanisms.

Wang, W Zhao, L Ni, B-J Yin, T-M Zhang, R-C Yu, M Shao, B Xu, X-J Xing, D-F Lee, D-J Ren, N-Q Chen, C

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Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Water Res, 2022, 222, pp. 118909
Issue Date:: 2022-08-15

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Field	Value	Language
dc.contributor.author	Wang, W
dc.contributor.author	Zhao, L
dc.contributor.author	Ni, B-J
dc.contributor.author	Yin, T-M
dc.contributor.author	Zhang, R-C
dc.contributor.author	Yu, M
dc.contributor.author	Shao, B
dc.contributor.author	Xu, X-J
dc.contributor.author	Xing, D-F
dc.contributor.author	Lee, D-J
dc.contributor.author	Ren, N-Q
dc.contributor.author	Chen, C
dc.date.accessioned	2023-03-19T21:13:33Z
dc.date.available	2022-07-25
dc.date.available	2023-03-19T21:13:33Z
dc.date.issued	2022-08-15
dc.identifier.citation	Water Res, 2022, 222, pp. 118909
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/167578
dc.description.abstract	Microbial denitrification is a crucial biological process for the treatment of nitrogen-polluted water. Traditional denitrification process consumes external organic carbon leading to an increase in treatment costs. We developed a novel sulfide-driven denitrification methane oxidation (SDMO) system that integrates autotrophic denitrification (AD) and denitrification anaerobic methane oxidation (DAMO) for cost-effective denitrification and biogas utilization in situ. Two SDMO systems were operated for 735 days, with nitrate and nitrite serving as electron acceptors, to explore the performance of sewage denitrification and characterize metabolic mechanisms. Results showed SDMO system could reach as high as 100% efficiency of nitrogen removal and biogas desulfurization without an external carbon source when HRT was 10 days and inflow nitrogen concentrations were 50-100 mgN·L-1. Besides, nitrate was a preferable electron acceptor for SDMO system. Biogas not only enhanced nitrogen removal but also intensified the DAMO, nitrogen removed through DAMO contribution doubled as original period from 2.9 mgN·(L·d)-1 to 6.2 mgN·(L·d)-1, and the ratio of nitrate removal through AD to DAMO was 1.2:1 with nitrate as electron acceptor. While nitrogen removed almost all through AD contribution and DAMO was weaken as before, the ratio of nitrate removal through AD to DAMO was 21.2:1 with nitrite as electron acceptor. Biogas introduced into SDMO system with nitrate inspired the growth of DAMO bacteria Candidatus Methylomirabilis from 0.3% to 19.6% and motivated its potentiality to remove nitrate without ANME archaea participation accompanying with gene mfnE upregulating ∼100 times. According to the reconstructed genome from binning analysis, the dramatically upregulated gene mfnE was derived from Candidatus Methylomirabilis, which may represent a novel metabolism pathway for DAMO bacteria to replace the role of archaea for nitrate reduction.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartof	Water Res
dc.relation.isbasedon	10.1016/j.watres.2022.118909
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Archaea
dc.subject.mesh	Bacteria
dc.subject.mesh	Biofuels
dc.subject.mesh	Bioreactors
dc.subject.mesh	Carbon
dc.subject.mesh	Denitrification
dc.subject.mesh	Methane
dc.subject.mesh	Nitrates
dc.subject.mesh	Nitrites
dc.subject.mesh	Nitrogen
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Sulfides
dc.subject.mesh	Bacteria
dc.subject.mesh	Archaea
dc.subject.mesh	Nitrates
dc.subject.mesh	Nitrites
dc.subject.mesh	Sulfides
dc.subject.mesh	Carbon
dc.subject.mesh	Nitrogen
dc.subject.mesh	Methane
dc.subject.mesh	Bioreactors
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Biofuels
dc.subject.mesh	Denitrification
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Archaea
dc.subject.mesh	Bacteria
dc.subject.mesh	Biofuels
dc.subject.mesh	Bioreactors
dc.subject.mesh	Carbon
dc.subject.mesh	Denitrification
dc.subject.mesh	Methane
dc.subject.mesh	Nitrates
dc.subject.mesh	Nitrites
dc.subject.mesh	Nitrogen
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Sulfides
dc.title	A novel sulfide-driven denitrification methane oxidation (SDMO) system: Operational performance and metabolic mechanisms.
dc.type	Journal Article
utslib.citation.volume	222
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-19T21:13:31Z
pubs.publication-status	Published
pubs.volume	222

Abstract:

Microbial denitrification is a crucial biological process for the treatment of nitrogen-polluted water. Traditional denitrification process consumes external organic carbon leading to an increase in treatment costs. We developed a novel sulfide-driven denitrification methane oxidation (SDMO) system that integrates autotrophic denitrification (AD) and denitrification anaerobic methane oxidation (DAMO) for cost-effective denitrification and biogas utilization in situ. Two SDMO systems were operated for 735 days, with nitrate and nitrite serving as electron acceptors, to explore the performance of sewage denitrification and characterize metabolic mechanisms. Results showed SDMO system could reach as high as 100% efficiency of nitrogen removal and biogas desulfurization without an external carbon source when HRT was 10 days and inflow nitrogen concentrations were 50-100 mgN·L-1. Besides, nitrate was a preferable electron acceptor for SDMO system. Biogas not only enhanced nitrogen removal but also intensified the DAMO, nitrogen removed through DAMO contribution doubled as original period from 2.9 mgN·(L·d)-1 to 6.2 mgN·(L·d)-1, and the ratio of nitrate removal through AD to DAMO was 1.2:1 with nitrate as electron acceptor. While nitrogen removed almost all through AD contribution and DAMO was weaken as before, the ratio of nitrate removal through AD to DAMO was 21.2:1 with nitrite as electron acceptor. Biogas introduced into SDMO system with nitrate inspired the growth of DAMO bacteria Candidatus Methylomirabilis from 0.3% to 19.6% and motivated its potentiality to remove nitrate without ANME archaea participation accompanying with gene mfnE upregulating ∼100 times. According to the reconstructed genome from binning analysis, the dramatically upregulated gene mfnE was derived from Candidatus Methylomirabilis, which may represent a novel metabolism pathway for DAMO bacteria to replace the role of archaea for nitrate reduction.

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http://hdl.handle.net/10453/167578