Biochar enhanced the performance of microalgae/bacteria consortium for insecticides removal from synthetic wastewater

Mojiri, A; Zhou, JL; Nazari V, M; Rezania, S; Farraji, H; Vakili, M

Biochar enhanced the performance of microalgae/bacteria consortium for insecticides removal from synthetic wastewater

Mojiri, A Zhou, JL Nazari V, M Rezania, S Farraji, H Vakili, M

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: Process Safety and Environmental Protection, 2022, 157, pp. 284-296
Issue Date:: 2022-01-01

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Field	Value	Language
dc.contributor.author	Mojiri, A
dc.contributor.author	Zhou, JL
dc.contributor.author	Nazari V, M
dc.contributor.author	Rezania, S
dc.contributor.author	Farraji, H
dc.contributor.author	Vakili, M
dc.date.accessioned	2023-03-20T04:44:34Z
dc.date.available	2023-03-20T04:44:34Z
dc.date.issued	2022-01-01
dc.identifier.citation	Process Safety and Environmental Protection, 2022, 157, pp. 284-296
dc.identifier.issn	0957-5820
dc.identifier.issn	1744-3598
dc.identifier.uri	http://hdl.handle.net/10453/167767
dc.description.abstract	The presence of pesticides in aquatic environments has threatened marine food resources, aquaculture, fisheries and human health; therefore, two most used insecticides were removed during this study. Two photobioreactors, including biochar and Chlorella vulgaris/activated sludge (reactor 1), and Chlorella vulgaris/activated sludge (reactor 2) were run to remove chlorpyrifos (CPF) and cypermethrin (CYP). Proteobacteria, Bacteroidetes and Chloroflexi were the dominant phyla of activated sludge. The optimization performance of both reactors was conducted by response surface methods. The performance of first photobioreactor was better than that in the second reactor, achieving abatement of 88.80% CPF and 93.12% CYP, at 69.7 h contact time and 0.32 mg/L initial concentration. The toxicity of CPF and CYP to Chlorella vulgaris was monitored under 0–4 mg/L of insecticide concentrations and 0–72 h contact time. The minimum chlorophyll content (2 mg/L) and protein (16.7%), and maximum growth inhibition (89.7%) were recorded at 4 mg/L insecticides concentration and 72 h contact time. Moreover, molecular docking simulation for catalytic enzyme degradation of Proteobacteria, Bacteroidetes and microalgae was carried out using individual hydrolase enzymes: carboxypeptidase in microalgae, isochorismatase hydrolase in Proteobacteria and alpha-L-arabinofuranosidase in Bacteroidetes. Ligand-binding energy, affinity and dimensions of ligands-binding sites in the enzyme cavity were calculated in each case. Hydrolase is an enzyme group that offers a promising practical application for the degradation of CYP and CPF due to its cavity features. This analysis demonstrated the mode of interaction of ligands with hydrolase enzymes in different species.
dc.language	English
dc.publisher	ELSEVIER
dc.relation.ispartof	Process Safety and Environmental Protection
dc.relation.isbasedon	10.1016/j.psep.2021.11.012
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject	0102 Applied Mathematics, 0904 Chemical Engineering, 0911 Maritime Engineering, 0914 Resources Engineering and Extractive Metallurgy
dc.subject.classification	Chemical Engineering
dc.subject.classification	Strategic, Defence & Security Studies
dc.title	Biochar enhanced the performance of microalgae/bacteria consortium for insecticides removal from synthetic wastewater
dc.type	Journal Article
utslib.citation.volume	157
utslib.for	0102 Applied Mathematics
utslib.for	0904 Chemical Engineering
utslib.for	0911 Maritime Engineering
utslib.for	0914 Resources Engineering and Extractive Metallurgy
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2024-01-01T00:00:00+1000Z
dc.date.updated	2023-03-20T04:44:33Z
pubs.publication-status	Published
pubs.volume	157

Abstract:

The presence of pesticides in aquatic environments has threatened marine food resources, aquaculture, fisheries and human health; therefore, two most used insecticides were removed during this study. Two photobioreactors, including biochar and Chlorella vulgaris/activated sludge (reactor 1), and Chlorella vulgaris/activated sludge (reactor 2) were run to remove chlorpyrifos (CPF) and cypermethrin (CYP). Proteobacteria, Bacteroidetes and Chloroflexi were the dominant phyla of activated sludge. The optimization performance of both reactors was conducted by response surface methods. The performance of first photobioreactor was better than that in the second reactor, achieving abatement of 88.80% CPF and 93.12% CYP, at 69.7 h contact time and 0.32 mg/L initial concentration. The toxicity of CPF and CYP to Chlorella vulgaris was monitored under 0–4 mg/L of insecticide concentrations and 0–72 h contact time. The minimum chlorophyll content (2 mg/L) and protein (16.7%), and maximum growth inhibition (89.7%) were recorded at 4 mg/L insecticides concentration and 72 h contact time. Moreover, molecular docking simulation for catalytic enzyme degradation of Proteobacteria, Bacteroidetes and microalgae was carried out using individual hydrolase enzymes: carboxypeptidase in microalgae, isochorismatase hydrolase in Proteobacteria and alpha-L-arabinofuranosidase in Bacteroidetes. Ligand-binding energy, affinity and dimensions of ligands-binding sites in the enzyme cavity were calculated in each case. Hydrolase is an enzyme group that offers a promising practical application for the degradation of CYP and CPF due to its cavity features. This analysis demonstrated the mode of interaction of ligands with hydrolase enzymes in different species.

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http://hdl.handle.net/10453/167767