Effect of elevated temperature on SARS-CoV-2 viability

Harapan, H; Johar, E; Maroef, CN; Sriyani, IY; Iqhrammullah, M; Kusuma, HI; Syukri, M; Razali, R; Hamdani, H; Kurniawan, R; Irwansyah, I; Sofyan, SE; Myint, KS; Mahlia, TMI; Rizal, S

Effect of elevated temperature on SARS-CoV-2 viability

Harapan, H Johar, E Maroef, CN Sriyani, IY Iqhrammullah, M Kusuma, HI Syukri, M Razali, R Hamdani, H Kurniawan, R Irwansyah, I Sofyan, SE Myint, KS Mahlia, TMI Rizal, S

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Publisher:: Faculty of 1000
Publication Type:: Journal Article
Citation:: F1000Research, 11, pp. 403

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Field	Value	Language
dc.contributor.author	Harapan, H
dc.contributor.author	Johar, E
dc.contributor.author	Maroef, CN
dc.contributor.author	Sriyani, IY
dc.contributor.author	Iqhrammullah, M
dc.contributor.author	Kusuma, HI
dc.contributor.author	Syukri, M
dc.contributor.author	Razali, R
dc.contributor.author	Hamdani, H
dc.contributor.author	Kurniawan, R
dc.contributor.author	Irwansyah, I
dc.contributor.author	Sofyan, SE
dc.contributor.author	Myint, KS
dc.contributor.author	Mahlia, TMI
dc.contributor.author	Rizal, S
dc.date.accessioned	2023-03-21T22:25:06Z
dc.date.available	2023-03-21T22:25:06Z
dc.identifier.citation	F1000Research, 11, pp. 403
dc.identifier.issn	2046-1402
dc.identifier.issn	2046-1402
dc.identifier.uri	http://hdl.handle.net/10453/167985
dc.description.abstract	<ns3:p>Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 10<ns3:sup>13</ns3:sup> PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 10<ns3:sup>13</ns3:sup> PFU/mL to 10<ns3:sup>9</ns3:sup> PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.</ns3:p>
dc.language	en
dc.publisher	Faculty of 1000
dc.relation.ispartof	F1000Research
dc.relation.isbasedon	10.12688/f1000research.110305.1
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology, 1103 Clinical Sciences, 1112 Oncology and Carcinogenesis
dc.title	Effect of elevated temperature on SARS-CoV-2 viability
dc.type	Journal Article
utslib.citation.volume	11
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1103 Clinical Sciences
utslib.for	1112 Oncology and Carcinogenesis
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	open_access	*
dc.date.updated	2023-03-21T22:25:05Z
pubs.publication-status	Published online
pubs.volume	11

Abstract:

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a worldwide disruption of global health putting healthcare workers at high risk. To reduce the transmission of SARS-CoV-2, in particular during treating the patients, our team aims to develop an optimized isolation chamber. The present study was conducted to evaluate the role of temperature elevation against SARS-CoV-2 viability, where the information would be used to build the isolation chamber. 0.6 mL of the Indonesian isolate of SARS-CoV-2 strain 20201012747 (approximately 1013 PFU/mL) was incubated for one hour with a variation of temperatures: 25, 30, 35, 40, 45, 50, 55, 60, and 65°C in digital block heater as well as at room temperature (21-23°C) before used to infect Vero E6 cells. The viability was determined using a plaque assay. Our data found a significant reduction of the viral viability from 1013 PFU/mL to 109 PFU/mL after the room temperature was increase to 40°C. Further elevation revealed that 55°C and above resulted in the total elimination of the viral viability. Increasing the temperature 40°C to reduce the SARS-CoV-2 survival could create mild hyperthermia conditions in a patient which could act as a thermotherapy. In addition, according to our findings, thermal sterilization of the vacant isolation chamber could be conducted by increasing the temperature to 55°C. In conclusion, elevating the temperature of the isolation chamber could be one of the main variables for developing an optimized isolation chamber for COVID-19 patients.

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http://hdl.handle.net/10453/167985