Different microplastics distinctively enriched the antibiotic resistance genes in anaerobic sludge digestion through shifting specific hosts and promoting horizontal gene flow.

Luo, T; Dai, X; Chen, Z; Wu, L; Wei, W; Xu, Q; Ni, B-J

Different microplastics distinctively enriched the antibiotic resistance genes in anaerobic sludge digestion through shifting specific hosts and promoting horizontal gene flow.

Luo, T Dai, X Chen, Z

Wu, L Wei, W Xu, Q Ni, B-J

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Water Res, 2023, 228, (Pt A), pp. 119356
Issue Date:: 2023-01-01

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Field	Value	Language
dc.contributor.author	Luo, T
dc.contributor.author	Dai, X
dc.contributor.author	Chen, Z https://orcid.org/0000-0003-0627-0856
dc.contributor.author	Wu, L
dc.contributor.author	Wei, W
dc.contributor.author	Xu, Q
dc.contributor.author	Ni, B-J
dc.date.accessioned	2023-03-22T01:05:39Z
dc.date.available	2022-11-12
dc.date.available	2023-03-22T01:05:39Z
dc.date.issued	2023-01-01
dc.identifier.citation	Water Res, 2023, 228, (Pt A), pp. 119356
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/168031
dc.description.abstract	Both microplastics (MPs) and antibiotic resistance genes (ARGs) are intensively detected in waste activated sludge (WAS). However, the distinctive impacts of different MPs on ARGs emergence, dissemination, and its potential mechanisms remain unclear. In this study, long-term semi-continuous digesters were performed to examine the profiles of ARGs and antibiotic-resistant bacteria (ARB) in response to two different typical MPs (polyethylene (PE) and polyvinyl chloride (PVC)) in anaerobic sludge digestion. Metagenomic results show that PE- and PVC-MPs increase ARGs abundance by 14.8% and 23.6% in digester, respectively. ARB are also enriched by PE- and PVC-MPs, Acinetobacter sp. and Salmonella sp. are the dominant ARB. Further exploration reveals that PVC-MPs stimulates the acquisition of ARGs by human pathogen bacteria (HPB) and functional microorganisms (FMs), but PE-MPs doesn't. Network analysis shows that more ARGs tend to co-occur with HBP and FMs after MPs exposure, and more importantly, new bacteria are observed to acquire ARGs possibly via horizontal gene flow (HGF) in MPs-stressed digester. The genes involved in the HGF process, including reactive oxygen species (ROS) production, cell membrane permeability, extracellular polymeric substances (EPS) secretion, and ATP synthesis, are also enhanced by MPs, thereby attributing to the promoted ARGs dissemination. These findings offer advanced insights into the distinctive contribution of MPs to fate, host, dissemination of ARGs in anaerobic sludge digestion.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation	http://purl.org/au-research/grants/arc/DP220101139
dc.relation	http://purl.org/au-research/grants/arc/DE220100530
dc.relation.ispartof	Water Res
dc.relation.isbasedon	10.1016/j.watres.2022.119356
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	Humans
dc.subject.mesh	Microplastics
dc.subject.mesh	Sewage
dc.subject.mesh	Gene Flow
dc.subject.mesh	Plastics
dc.subject.mesh	Polyvinyl Chloride
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Angiotensin Receptor Antagonists
dc.subject.mesh	Angiotensin-Converting Enzyme Inhibitors
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Polyethylene
dc.subject.mesh	Digestion
dc.subject.mesh	Humans
dc.subject.mesh	Polyethylene
dc.subject.mesh	Polyvinyl Chloride
dc.subject.mesh	Plastics
dc.subject.mesh	Angiotensin-Converting Enzyme Inhibitors
dc.subject.mesh	Sewage
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Digestion
dc.subject.mesh	Gene Flow
dc.subject.mesh	Angiotensin Receptor Antagonists
dc.subject.mesh	Microplastics
dc.subject.mesh	Humans
dc.subject.mesh	Microplastics
dc.subject.mesh	Sewage
dc.subject.mesh	Gene Flow
dc.subject.mesh	Plastics
dc.subject.mesh	Polyvinyl Chloride
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Angiotensin Receptor Antagonists
dc.subject.mesh	Angiotensin-Converting Enzyme Inhibitors
dc.subject.mesh	Drug Resistance, Microbial
dc.subject.mesh	Polyethylene
dc.subject.mesh	Digestion
dc.title	Different microplastics distinctively enriched the antibiotic resistance genes in anaerobic sludge digestion through shifting specific hosts and promoting horizontal gene flow.
dc.type	Journal Article
utslib.citation.volume	228
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-22T01:05:35Z
pubs.issue	Pt A
pubs.publication-status	Published
pubs.volume	228
utslib.citation.issue	Pt A

Abstract:

Both microplastics (MPs) and antibiotic resistance genes (ARGs) are intensively detected in waste activated sludge (WAS). However, the distinctive impacts of different MPs on ARGs emergence, dissemination, and its potential mechanisms remain unclear. In this study, long-term semi-continuous digesters were performed to examine the profiles of ARGs and antibiotic-resistant bacteria (ARB) in response to two different typical MPs (polyethylene (PE) and polyvinyl chloride (PVC)) in anaerobic sludge digestion. Metagenomic results show that PE- and PVC-MPs increase ARGs abundance by 14.8% and 23.6% in digester, respectively. ARB are also enriched by PE- and PVC-MPs, Acinetobacter sp. and Salmonella sp. are the dominant ARB. Further exploration reveals that PVC-MPs stimulates the acquisition of ARGs by human pathogen bacteria (HPB) and functional microorganisms (FMs), but PE-MPs doesn't. Network analysis shows that more ARGs tend to co-occur with HBP and FMs after MPs exposure, and more importantly, new bacteria are observed to acquire ARGs possibly via horizontal gene flow (HGF) in MPs-stressed digester. The genes involved in the HGF process, including reactive oxygen species (ROS) production, cell membrane permeability, extracellular polymeric substances (EPS) secretion, and ATP synthesis, are also enhanced by MPs, thereby attributing to the promoted ARGs dissemination. These findings offer advanced insights into the distinctive contribution of MPs to fate, host, dissemination of ARGs in anaerobic sludge digestion.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/168031