Insights into N2O turnovers under polyethylene terephthalate microplastics stress in mainstream biological nitrogen removal process.

He, Y; Liu, Y; Yan, M; Zhao, T; Liu, Y; Zhu, T; Ni, B-J

Insights into N2O turnovers under polyethylene terephthalate microplastics stress in mainstream biological nitrogen removal process.

He, Y Liu, Y Yan, M Zhao, T Liu, Y Zhu, T Ni, B-J

Permalink

Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Water Res, 2022, 224, pp. 119037
Issue Date:: 2022-10-01

Closed Access

	Filename	Description	Size
	Insights into N2O turnovers.pdf		6.26 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	He, Y
dc.contributor.author	Liu, Y
dc.contributor.author	Yan, M
dc.contributor.author	Zhao, T
dc.contributor.author	Liu, Y
dc.contributor.author	Zhu, T
dc.contributor.author	Ni, B-J
dc.date.accessioned	2023-03-23T02:06:19Z
dc.date.available	2022-08-28
dc.date.available	2023-03-23T02:06:19Z
dc.date.issued	2022-10-01
dc.identifier.citation	Water Res, 2022, 224, pp. 119037
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/168151
dc.description.abstract	The ubiquitous microplastics in wastewater have raised growing concerns due to their unintended effects on microbial activities. However, whether and how microplastics affect nitrous oxide (N2O) (a potent greenhouse gas) turnovers in mainstream biological nitrogen removal (BNR) process remain unclear. This work therefore aimed to fill such knowledge gap by conducting both long-term and batch tests. After over 100 days of feeding with wastewater containing polyethylene terephthalate (PET) microplastics (0-500 μg/L), the long-term results showed that both production and reduction of N2O during denitrification were reduced, as well as the N2O production during nitrification. Accordingly, 60% reduction in N2O accumulation and 70% reduction in N2O production were observed in the denitrification and nitrification batch tests, respectively. Nevertheless, the long-term N2O emission factors under PET microplastics stress were comparable to that in the control reactor, mainly because PET microplastics led to more nitrite accumulation in anoxic period. With the aid of online N2O sensors and site-preference analysis, it was demonstrated that the heterotrophic bacteria pathway and ammonia oxidizing bacteria denitrification pathway for N2O production were negatively affected by PET microplastics, whereas a clear increase in the contribution of hydroxylamine pathway (+ 22.9%) was observed. Further investigation revealed that PET microplastics even at environmental level (i.e. 10 μg/L) significantly reshaped the BNR sludge characteristics (e.g. much larger particle size) and microbial communities (e.g. Thauera, Rhodobacte and Nitrospira) as well as the nitrogen metabolism pathways, which were chiefly responsible for the changes of N2O turnovers and N2O production pathways under the PET microplastics stress.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation.ispartof	Water Res
dc.relation.isbasedon	10.1016/j.watres.2022.119037
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	Ammonia
dc.subject.mesh	Bioreactors
dc.subject.mesh	Denitrification
dc.subject.mesh	Greenhouse Gases
dc.subject.mesh	Hydroxylamines
dc.subject.mesh	Microplastics
dc.subject.mesh	Nitrification
dc.subject.mesh	Nitrites
dc.subject.mesh	Nitrogen
dc.subject.mesh	Nitrous Oxide
dc.subject.mesh	Plastics
dc.subject.mesh	Polyethylene Terephthalates
dc.subject.mesh	Sewage
dc.subject.mesh	Wastewater
dc.subject.mesh	Nitrites
dc.subject.mesh	Nitrogen
dc.subject.mesh	Ammonia
dc.subject.mesh	Nitrous Oxide
dc.subject.mesh	Hydroxylamines
dc.subject.mesh	Plastics
dc.subject.mesh	Polyethylene Terephthalates
dc.subject.mesh	Bioreactors
dc.subject.mesh	Sewage
dc.subject.mesh	Denitrification
dc.subject.mesh	Nitrification
dc.subject.mesh	Greenhouse Gases
dc.subject.mesh	Microplastics
dc.subject.mesh	Wastewater
dc.subject.mesh	Ammonia
dc.subject.mesh	Bioreactors
dc.subject.mesh	Denitrification
dc.subject.mesh	Greenhouse Gases
dc.subject.mesh	Hydroxylamines
dc.subject.mesh	Microplastics
dc.subject.mesh	Nitrification
dc.subject.mesh	Nitrites
dc.subject.mesh	Nitrogen
dc.subject.mesh	Nitrous Oxide
dc.subject.mesh	Plastics
dc.subject.mesh	Polyethylene Terephthalates
dc.subject.mesh	Sewage
dc.subject.mesh	Wastewater
dc.title	Insights into N2O turnovers under polyethylene terephthalate microplastics stress in mainstream biological nitrogen removal process.
dc.type	Journal Article
utslib.citation.volume	224
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access	*
dc.date.updated	2023-03-23T02:06:02Z
pubs.publication-status	Published
pubs.volume	224

Abstract:

The ubiquitous microplastics in wastewater have raised growing concerns due to their unintended effects on microbial activities. However, whether and how microplastics affect nitrous oxide (N2O) (a potent greenhouse gas) turnovers in mainstream biological nitrogen removal (BNR) process remain unclear. This work therefore aimed to fill such knowledge gap by conducting both long-term and batch tests. After over 100 days of feeding with wastewater containing polyethylene terephthalate (PET) microplastics (0-500 μg/L), the long-term results showed that both production and reduction of N2O during denitrification were reduced, as well as the N2O production during nitrification. Accordingly, 60% reduction in N2O accumulation and 70% reduction in N2O production were observed in the denitrification and nitrification batch tests, respectively. Nevertheless, the long-term N2O emission factors under PET microplastics stress were comparable to that in the control reactor, mainly because PET microplastics led to more nitrite accumulation in anoxic period. With the aid of online N2O sensors and site-preference analysis, it was demonstrated that the heterotrophic bacteria pathway and ammonia oxidizing bacteria denitrification pathway for N2O production were negatively affected by PET microplastics, whereas a clear increase in the contribution of hydroxylamine pathway (+ 22.9%) was observed. Further investigation revealed that PET microplastics even at environmental level (i.e. 10 μg/L) significantly reshaped the BNR sludge characteristics (e.g. much larger particle size) and microbial communities (e.g. Thauera, Rhodobacte and Nitrospira) as well as the nitrogen metabolism pathways, which were chiefly responsible for the changes of N2O turnovers and N2O production pathways under the PET microplastics stress.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/168151