Effects of voltage on the emergence and spread of antibiotic resistance genes in microbial electrolysis cells: From mutation to horizontal gene transfer.
- Publisher:
- PERGAMON-ELSEVIER SCIENCE LTD
- Publication Type:
- Journal Article
- Citation:
- Chemosphere, 2022, 291, (Pt 1), pp. 132703-132703
- Issue Date:
- 2022-03-01
Closed Access
Filename | Description | Size | |||
---|---|---|---|---|---|
Effects of voltage on the emergence and spread of antibiotic resistance genes in microbial electrolysis cells From mutation to horizontal gene transfer..pdf | Published version | 7.78 MB | Adobe PDF |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Zhang, S | |
dc.contributor.author | Sun, W-L | |
dc.contributor.author | Song, H-L | |
dc.contributor.author | Zhang, T | |
dc.contributor.author | Yin, M | |
dc.contributor.author |
Wang, Q https://orcid.org/0000-0002-5744-2331 |
|
dc.contributor.author | Zuo, X | |
dc.date.accessioned | 2023-03-23T03:00:23Z | |
dc.date.available | 2021-10-24 | |
dc.date.available | 2023-03-23T03:00:23Z | |
dc.date.issued | 2022-03-01 | |
dc.identifier.citation | Chemosphere, 2022, 291, (Pt 1), pp. 132703-132703 | |
dc.identifier.issn | 0045-6535 | |
dc.identifier.issn | 1879-1298 | |
dc.identifier.uri | http://hdl.handle.net/10453/168176 | |
dc.description.abstract | Microbial electrolysis cells (MECs) are widely considered as promising alternatives for degrading antibiotics. As one of the major operating parameters in MECs, voltage might affect the spread of antibiotic resistance genes (ARGs) given it can affect the physiological characteristics of bacteria. However, little is known about the impacts of voltage on the acceleration of bacterial mutation and the promotion of ARG dissemination via horizontal transfer in MECs. In this study, two voltages (0.9 V and 1.5 V) were applied to identify if electrical stimulation could increase bacterial mutation frequency. Three voltages (0.9 V, 1.5 V, and 2.5 V) were used to evaluate the conjugative transfer frequency of plasmid-encoded the ARGs from the donor (E. coli K-12) to the recipient (E. coli HB101) in MECs. After repeating subculture in MECs for 10 days, the mutation frequency of E. coli K-12 was promoted, consequently, the generated mutants became more resistant against tetracycline. When the voltage was higher than 0.9 V, conjugative ARG transfer frequency was significantly increased in the anode chamber (p < 0.05). The over-production of reactive oxygen species (ROS) (voltage >0.9 V) and cell membrane permeability (voltage >1.5 V) were significantly enhanced under electrical stimulations (p < 0.05). Genome-wide RNA sequencing indicated that the expressions of genes related to oxidative stress and cell membrane were upregulated with exposure to electrical stimulation. Electrical stimulations induced oxidative reactions, which triggered ROS over-production, SOS response, and enhancement of cell membrane permeability for both donor and recipient in the MECs. These findings provide insights into the potential role of voltage in the generation and spread of ARGs in MECs. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | |
dc.relation.ispartof | Chemosphere | |
dc.relation.isbasedon | 10.1016/j.chemosphere.2021.132703 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject.classification | Environmental Sciences | |
dc.subject.classification | Meteorology & Atmospheric Sciences | |
dc.subject.mesh | Anti-Bacterial Agents | |
dc.subject.mesh | Drug Resistance, Microbial | |
dc.subject.mesh | Electrolysis | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Gene Transfer, Horizontal | |
dc.subject.mesh | Genes, Bacterial | |
dc.subject.mesh | Mutation | |
dc.subject.mesh | Anti-Bacterial Agents | |
dc.subject.mesh | Drug Resistance, Microbial | |
dc.subject.mesh | Electrolysis | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Gene Transfer, Horizontal | |
dc.subject.mesh | Genes, Bacterial | |
dc.subject.mesh | Mutation | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Anti-Bacterial Agents | |
dc.subject.mesh | Drug Resistance, Microbial | |
dc.subject.mesh | Gene Transfer, Horizontal | |
dc.subject.mesh | Mutation | |
dc.subject.mesh | Genes, Bacterial | |
dc.subject.mesh | Electrolysis | |
dc.subject.mesh | Anti-Bacterial Agents | |
dc.subject.mesh | Drug Resistance, Microbial | |
dc.subject.mesh | Electrolysis | |
dc.subject.mesh | Escherichia coli | |
dc.subject.mesh | Gene Transfer, Horizontal | |
dc.subject.mesh | Genes, Bacterial | |
dc.subject.mesh | Mutation | |
dc.title | Effects of voltage on the emergence and spread of antibiotic resistance genes in microbial electrolysis cells: From mutation to horizontal gene transfer. | |
dc.type | Journal Article | |
utslib.citation.volume | 291 | |
utslib.location.activity | England | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering | |
pubs.organisational-group | /University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment | |
utslib.copyright.status | closed_access | * |
pubs.consider-herdc | false | |
dc.date.updated | 2023-03-23T03:00:22Z | |
pubs.issue | Pt 1 | |
pubs.publication-status | Published | |
pubs.volume | 291 | |
utslib.citation.issue | Pt 1 |
Abstract:
Microbial electrolysis cells (MECs) are widely considered as promising alternatives for degrading antibiotics. As one of the major operating parameters in MECs, voltage might affect the spread of antibiotic resistance genes (ARGs) given it can affect the physiological characteristics of bacteria. However, little is known about the impacts of voltage on the acceleration of bacterial mutation and the promotion of ARG dissemination via horizontal transfer in MECs. In this study, two voltages (0.9 V and 1.5 V) were applied to identify if electrical stimulation could increase bacterial mutation frequency. Three voltages (0.9 V, 1.5 V, and 2.5 V) were used to evaluate the conjugative transfer frequency of plasmid-encoded the ARGs from the donor (E. coli K-12) to the recipient (E. coli HB101) in MECs. After repeating subculture in MECs for 10 days, the mutation frequency of E. coli K-12 was promoted, consequently, the generated mutants became more resistant against tetracycline. When the voltage was higher than 0.9 V, conjugative ARG transfer frequency was significantly increased in the anode chamber (p < 0.05). The over-production of reactive oxygen species (ROS) (voltage >0.9 V) and cell membrane permeability (voltage >1.5 V) were significantly enhanced under electrical stimulations (p < 0.05). Genome-wide RNA sequencing indicated that the expressions of genes related to oxidative stress and cell membrane were upregulated with exposure to electrical stimulation. Electrical stimulations induced oxidative reactions, which triggered ROS over-production, SOS response, and enhancement of cell membrane permeability for both donor and recipient in the MECs. These findings provide insights into the potential role of voltage in the generation and spread of ARGs in MECs.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph